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Metagenomic Assembly: Reconstructing Genomes from Metagenomes
2021Assembly of metagenomic sequence data into microbial genomes is of critical importance for disentangling community complexity and unraveling the functional capacity of microorganisms. The rapid development of sequencing technology and novel assembly algorithms have made it possible to reliably reconstruct hundreds to thousands of microbial genomes from
Wang Z. +4 more
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2017
Thanks in large part to newer, better, and cheaper DNA sequencing technologies, an enormous number of metagenomic sequence datasets have been and continue to be generated, covering a huge variety of environmental niches, including several different human body sites.
Andrew, Maltez Thomas +5 more
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Thanks in large part to newer, better, and cheaper DNA sequencing technologies, an enormous number of metagenomic sequence datasets have been and continue to be generated, covering a huge variety of environmental niches, including several different human body sites.
Andrew, Maltez Thomas +5 more
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Nature Reviews Microbiology, 2005
Viruses, most of which infect microorganisms, are the most abundant biological entities on the planet. Identifying and measuring the community dynamics of viruses in the environment is complicated because less than one percent of microbial hosts have been cultivated. Also, there is no single gene that is common to all viral genomes, so total uncultured
Robert A, Edwards, Forest, Rohwer
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Viruses, most of which infect microorganisms, are the most abundant biological entities on the planet. Identifying and measuring the community dynamics of viruses in the environment is complicated because less than one percent of microbial hosts have been cultivated. Also, there is no single gene that is common to all viral genomes, so total uncultured
Robert A, Edwards, Forest, Rohwer
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Metagenomics for Bioremediation
2011info:eu-repo/semantics ...
George, Isabelle +2 more
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2017
Metagenomics approach involves direct genetic analysis of environmental samples, evading the tedious culturing process. Polymerase chain reaction is one invaluable tool used for such analyses. Here, we describe one protocol for metagenomic DNA isolation that gives inhibitor-free DNA suitable for PCR and other genetic manipulations.
Tina Kollannoor, Johny +1 more
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Metagenomics approach involves direct genetic analysis of environmental samples, evading the tedious culturing process. Polymerase chain reaction is one invaluable tool used for such analyses. Here, we describe one protocol for metagenomic DNA isolation that gives inhibitor-free DNA suitable for PCR and other genetic manipulations.
Tina Kollannoor, Johny +1 more
openaire +2 more sources
2018
This project is focusing on deciphering the functional complexity of foxtail millet root microbiota using ultra-deep metagenomic sequencing and identifying biomarkers for millet yield.
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This project is focusing on deciphering the functional complexity of foxtail millet root microbiota using ultra-deep metagenomic sequencing and identifying biomarkers for millet yield.
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Metagenomic signatures of 86 microbial and viral metagenomes
Environmental Microbiology, 2009Summary Previous studies have shown that dinucleotide abundances capture the majority of variation in genome signatures and are useful for quantifying lateral gene transfer and building molecular phylogenies. Metagenomes contain a mixture of individual genomes, and might be expected to lack compositional signatures.
Willner, Dana +2 more
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[Microbiotes and metagenomics].
Medecine sciences : M/S, 2016Major technical advances were introduced in the study of microflora and microbial communities in the nineties. These are essentially analytical approaches conducted as frequently by brute force. What conclusions can be drawn from these analyses twenty years later? In terms of microbial compositions, monitoring and diagnosis, the results are impressive.
Weissenbach, Jean, Sghir, Abdelghani
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Nature Reviews Microbiology, 2005
Phylogenetic surveys of soil ecosystems have shown that the number of prokaryotic species found in a single sample exceeds that of known cultured prokaryotes. Soil metagenomics, which comprises isolation of soil DNA and the production and screening of clone libraries, can provide a cultivation-independent assessment of the largely untapped genetic ...
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Phylogenetic surveys of soil ecosystems have shown that the number of prokaryotic species found in a single sample exceeds that of known cultured prokaryotes. Soil metagenomics, which comprises isolation of soil DNA and the production and screening of clone libraries, can provide a cultivation-independent assessment of the largely untapped genetic ...
openaire +2 more sources