The unseen world: environmental microbial sequencing and identification methods for ecologists [PDF]
Archaea, bacteria, microeukaryotes, and the viruses that infect them (collectively “microorganisms”) are foundational components of all ecosystems, inhabiting almost every imaginable environment and comprising the majority of the planet’s organismal and ...
Aronson, Emma+4 more
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Promises and Prospects of Microbiome Studies [PDF]
Since Anthony van Leeuwenhoek, first microscopic observations of the unseen microbiota and the more recent realization that little of the microbes in the biosphere are known, humans have developed a deep curiosity to fully understand the inner workings ...
Izard, Jacques, Rivera, Maria C.
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Deconvolute individual genomes from metagenome sequences through short read clustering. [PDF]
Metagenome assembly from short next-generation sequencing data is a challenging process due to its large scale and computational complexity. Clustering short reads by species before assembly offers a unique opportunity for parallel downstream assembly of
Deng, Li+5 more
core
Construction and Screening of Metagenomic Libraries Derived from Enrichment Cultures: Generation of a Gene Bank for Genes Conferring Alcohol Oxidoreductase Activity on Escherichia coli [PDF]
Anja Knietsch+4 more
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Identification and Characterization of Coenzyme B 12 -Dependent Glycerol Dehydratase- and Diol Dehydratase-Encoding Genes from Metagenomic DNA Libraries Derived from Enrichment Cultures [PDF]
Anja Knietsch+4 more
openalex +1 more source
Case study of the intestinal microbiota using the XGN-MBI metagenomic assay [PDF]
The microbiota of the colon and rectum is the most abundant and diverse of the human body, with a density of up to 1-2 kg of weight and a diversity that exceeds a thousand species.
Burgos-Molina, Antonio Manuel+4 more
core
Metagenomics: DNA sequencing of environmental samples [PDF]
Susannah G. Tringe, Edward M. Rubin
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Metagenomics for studying unculturable microorganisms: cutting the Gordian knot. [PDF]
Patrick D. Schloss, Jo Handelsman
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The role of lipopolysaccharide (LPS) in the development of diseases is clear, but the specific mechanisms remain poorly understood. This study aimed to investigate the microbiome aberrations in the guts of mice against the background of LPS, as well as ...
Mariya Gryaznova+8 more
doaj +1 more source
Optimizing sequencing protocols for leaderboard metagenomics by combining long and short reads. [PDF]
As metagenomic studies move to increasing numbers of samples, communities like the human gut may benefit more from the assembly of abundant microbes in many samples, rather than the exhaustive assembly of fewer samples.
Arthur, Timothy D+30 more
core +1 more source