Results 41 to 50 of about 117,934 (343)

Giant virus diversity and host interactions through global metagenomics

open access: yesNature, 2020
Our current knowledge about nucleocytoplasmic large DNA viruses (NCLDVs) is largely derived from viral isolates that are co-cultivated with protists and algae.
Frederik Schulz   +10 more
semanticscholar   +1 more source

Validation and standardization of DNA extraction and library construction methods for metagenomics-based human fecal microbiome measurements

open access: yesMicrobiome, 2021
Background Validation and standardization of methodologies for microbial community measurements by high-throughput sequencing are needed to support human microbiome research and its industrialization.
Dieter M. Tourlousse   +34 more
semanticscholar   +1 more source

Benchmark of 16S rRNA gene amplicon sequencing using Japanese gut microbiome data from the V1–V2 and V3–V4 primer sets

open access: yesBMC Genomics, 2021
Background 16S rRNA gene amplicon sequencing (16S analysis) is widely used to analyze microbiota with next-generation sequencing technologies. Here, we compared fecal 16S analysis data from 192 Japanese volunteers using the modified V1–V2 (V12) and the ...
Shoichiro Kameoka   +9 more
doaj   +1 more source

Genomic Characterization of a Prophage, Smhb1, That Infects Salinivibrio kushneri BNH Isolated from a Namib Desert Saline Spring

open access: yesMicroorganisms, 2021
Recent years have seen the classification and reclassification of many viruses related to the model enterobacterial phage P2. Here, we report the identification of a prophage (Smhb1) that infects Salinivibrio kushneri BNH isolated from a Namib Desert ...
Israel Olonade   +2 more
doaj   +1 more source

Hot Spring Metagenomics [PDF]

open access: yesLife, 2013
Hot springs have been investigated since the XIX century, but isolation and examination of their thermophilic microbial inhabitants did not start until the 1950s. Many thermophilic microorganisms and their viruses have since been discovered, although the real complexity of thermal communities was envisaged when research based on PCR amplification of ...
Olalla López-López   +2 more
openaire   +4 more sources

Integrating Viral Metagenomics into an Ecological Framework.

open access: yesAnnual Review of Virology, 2021
Viral metagenomics has expanded our knowledge of the ecology of uncultured viruses, within both environmental (e.g., terrestrial and aquatic) and host-associated (e.g., plants and animals, including humans) contexts. Here, we emphasize the implementation
P. Sommers   +3 more
semanticscholar   +1 more source

Investigating Colonization of the Healthy Adult Gastrointestinal Tract by Fungi

open access: yesmSphere, 2018
A wide diversity of fungi have been detected in the human gastrointestinal (GI) tract with the potential to provide or influence important functions. However, many of the fungi most commonly detected in stool samples are also present in food or the oral ...
Thomas A. Auchtung   +8 more
doaj   +1 more source

Molecular Phenomics and Metagenomics of Hepatic Steatosis in Non-Diabetic Obese Women

open access: yesNature Network Boston, 2018
Hepatic steatosis is a multifactorial condition that is often observed in obese patients and is a prelude to non-alcoholic fatty liver disease. Here, we combine shotgun sequencing of fecal metagenomes with molecular phenomics (hepatic transcriptome and ...
L. Hoyles   +34 more
semanticscholar   +1 more source

Recovery of strain-resolved genomes from human microbiome through an integration framework of single-cell genomics and metagenomics

open access: yesMicrobiome, 2021
Obtaining high-quality (HQ) reference genomes from microbial communities is crucial for understanding the phylogeny and function of uncultured microbes in complex microbial ecosystems.
Koji Arikawa   +8 more
semanticscholar   +1 more source

Enhanced mitochondrial activity reshapes a gut microbiota profile that delays NASH progression

open access: yesHepatology, EarlyView., 2022
Improved mitochondrial activity, due to the lack of methylation‐controlled J protein (MCJ), creates a specific microbiota signature that when transferred through cecal microbiota transplantation delays NASH progression by restoring the gut‐liver axis and enhancing hepatic fatty acid oxidation.
María Juárez‐Fernández   +18 more
wiley   +1 more source

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