Results 51 to 60 of about 4,878,195 (347)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

open access: yesFEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq   +3 more
wiley   +1 more source

Metal nanoparticles: understanding the mechanisms behind antibacterial activity

open access: yesJournal of Nanobiotechnology, 2017
As the field of nanomedicine emerges, there is a lag in research surrounding the topic of nanoparticle (NP) toxicity, particularly concerned with mechanisms of action. The continuous emergence of bacterial resistance has challenged the research community
Y. Slavin   +3 more
semanticscholar   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

open access: yesFEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso   +4 more
wiley   +1 more source

THE EFFECT OF REPETITIVE METAL CASTING ON THE TENSILE STRENGTH OF DENTURES

open access: yesJournal of Vocational Health Studies
Background: Dentures or prostheses function to replace chewing and dental arch structures. Denture plates used in dentistry are made with acrylic resin, metal, or a combination of acrylic and metal.
Sri Wiwik Wiyanti   +4 more
doaj   +1 more source

Phytoremediation: A Promising Approach for Revegetation of Heavy Metal-Polluted Land

open access: yesFrontiers in Plant Science, 2020
Heavy metal accumulation in soil has been rapidly increased due to various natural processes and anthropogenic (industrial) activities. As heavy metals are non-biodegradable, they persist in the environment, have potential to enter the food chain through
An Yan   +5 more
semanticscholar   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

Development of an efficient system for predicting CRSS ratios of HCP polycrystalline metals using uniaxial loading conditions

open access: yesNihon Kikai Gakkai ronbunshu
Critical resolved shear stress (CRSS) is one of the most important parameters in the field of plasticity in metals, and the CRSS ratios between slip systems govern the plastic anisotropy, workability, and so on.
Sotaro MIKAMI   +2 more
doaj   +1 more source

Feasibility and Tolerability of Performing Portable MRI for Neurological Disorders in an Outpatient Neurology Clinic: A Prospective Cohort

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Accessing brain magnetic resonance imaging (MRI) can be challenging, especially for underserved patients, which may lead to disparities in neurological diagnosis. Method This mixed‐methods study enrolled adults with one of four neurological disorders: mild cognitive impairment or dementia of the Alzheimer type, multiple sclerosis ...
Maya L. Mastick   +19 more
wiley   +1 more source

Low friction and wear characteristics formed by mechanically induced surface nanocrystallization: A review of recent advances

open access: yesFriction
Friction and wear of metallic materials are ubiquitous in modern life, resulting in significant energy consumption and property damage. The tribological performance of metallic materials is strongly affected by their surface characteristics, motivating ...
Youwang Tu   +3 more
doaj   +1 more source

Effect of Foot Orthoses on Midfoot Pain and the Volume of Bone Marrow Lesions in the Midfoot: A Randomized Mechanism of Action Study

open access: yesArthritis Care &Research, EarlyView.
Objective Foot orthoses are thought to improve pain by potentially modifying internal mechanical forces. To test this, we explored whether foot orthoses can modify patterns of bone marrow lesions (BMLs) in people with midfoot pain. Methods Forty‐two people were recruited with midfoot pain, and magnetic resonance imaging–confirmed midfoot BMLs ...
Jill Halstead   +4 more
wiley   +1 more source

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