Results 91 to 100 of about 411,479 (310)

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Fabrication of crystals from single metal atoms [PDF]

, 2014
Metal nanocrystals offer new concepts for the design of nanodevices with a range of potential applications. Currently the formation of metal nanocrystals cannot be controlled at the level of individual atoms. Here we describe a new general method for the
Smith, C.J., Beanland, R., Beanland, Richard, Hands-Portman, I., Nigel Kirby, Smith, Corinne J., Sánchez, Ana M., Dove, Andrew P., Rachel K. O’reilly, Corinne J. Smith, Joan J. Soldevila-barreda, O'Reilly, Rachel K.; id_orcid, Hands-Portman, Ian, Peter J. Sadler, O'Reilly, Rachel K., Ana M. Sanchez, Andrew P. Dove, Sanchez, A.M., O'Reilly, R.K., Richard J. Procter, Pitto-Barry, Anaïs, Kirby, Nigel, Sadler, P. J., Procter, R.J., Ian H, Sadler, P.J., Nicolas P. E. Barry, Soldevila-Barreda, Joan J., Sadler, Peter J., Procter, Richard J., Barry, Nicolas P. E., Richard Beanl, Kirby, N., Barry, Nicolas P.E., Dove, A.P.   +34 more
core   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Transference of Heat in Cooled Metal [PDF]

Nature, 1898
MY attention has just been called to two communications to your journal, entitled “Transference of Heat in Cooled Metal.” The first, by M. Henry Bourget, appears in the issue of June 30, and the second, by Mr. Albert T. Bartlett, in the issue of September 1.
openaire   +4 more sources

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Simulation Study of Surface Transfer Doping of Hydrogenated Diamond by MoO₃ and V₂O₅ Metal Oxides

Micromachines, 2020
In this work, we investigate the surface transfer doping process that is induced between hydrogen-terminated (100) diamond and the metal oxides, MoO3 and V2O5, through simulation using a semi-empirical Density Functional Theory (DFT) method. DFT was used
Joseph McGhee, Vihar P. Georgiev
doaj   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Numerical Investigation of Methane Steam Reforming in the Packed Bed Installed with Metal Foam

Chemical Engineering Transactions, 2023
Hydrogen is one of the vital energy sources for addressing climate change, and methane steam reforming via a packed bed reactor is a primary method for global hydrogen supply.
Zhihong Wu, Jian Yang, Qiuwang Wang
doaj  

Phase change convective heat transfer in high porosity cellular metal foams

, 2013
This Chapter discusses phase change convective heat transfer of high porosity cellular metal foams and their practical applications in thermal energy storage (TES).
Zhao, Changying, Tian, Yuan, Wang, Qian
core  

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source