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Prediction and Consequences of Cofragmentation in Metaproteomics
Journal of Proteome Research, 2019Metaproteomics can provide critical information about biological systems, but peptides are found within a complex background of other peptides. This complex background can change across samples, in some cases drastically. Cofragmentation, the coelution of peptides with similar mass to charge ratios, is one factor that influences which peptides are ...
J. Scott P. McCain, Erin M. Bertrand
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The Unipept metaproteomics analysis pipeline
PROTEOMICS, 2015Unipept ( http://unipept.ugent.be ) is a web application that offers a user‐friendly way to explore the biodiversity of complex metaproteome samples by providing interactive visualizations. In this article, the updates and changes to Unipept since its initial release are presented.
Bart, Mesuere +5 more
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Perspective and Guidelines for Metaproteomics in Microbiome Studies
Journal of Proteome Research, 2019The microbiome is emerging as a prominent factor affecting human health, and its dysbiosis is associated with various diseases. Compositional profiling of microbiome is increasingly being supplemented with functional characterization. Metaproteomics is intrinsically focused on functional changes and therefore will be an important tool in those studies ...
Xu Zhang, Daniel Figeys, Xu Zhang
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Interactive Chord Visualization for Metaproteomics
2017 28th International Workshop on Database and Expert Systems Applications (DEXA), 2017Metaproteomics is an analytic approach to research microorganisms that live in complex microbial communities. A key aspect of understanding microbial communities is to link the functions of proteins identified by metaproteomics to their taxonomy. In this paper we demonstrate the interactive chord visualization as a powerful tool to explore such data ...
Roman Zoun +5 more
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Metaproteomics Analysis of Host–Microbiota Interfaces
2021Metaproteomics of host-microbiome interfaces comprises the analysis of complex mixtures of bacteria, archaea, fungi, and viruses in combination with its host cells. Microbial niches can be found all over the host including the skin, oral cavity, and the intestine and are considered to be essential for the homeostasis.
Sjoerd, van der Post, Liisa, Arike
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Data-independent acquisition in metaproteomics
Expert Review of ProteomicsMetaproteomics offers insights into the function of complex microbial communities, while it is also capable of revealing microbe-microbe and host-microbe interactions. Data-independent acquisition (DIA) mass spectrometry is an emerging technology, which holds great potential to achieve deep and accurate metaproteomics with higher reproducibility yet ...
Enhui Wu +3 more
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Metaproteomics Study of the Gut Microbiome
2018Proteomics is a widely used method for defining the protein composition of a complex sample. As this approach allows for identification and quantification of proteins across a broad dynamic range as well as detection of post-translational modifications, proteomics is an ideal platform to investigate the gut microbiome at a functional level.
Lisa A, Lai +3 more
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Metaproteome and metabolome of oral microbial communities
Periodontology 2000, 2020AbstractThe emergence of high‐throughput technologies for the comprehensive measurement of biomolecules, also referred to as “omics” technologies, has helped us gather “big data” and characterize microbial communities. In this article, we focus on metaproteomic and metabolomic approaches that support hypothesis‐driven investigations on various oral ...
Bostanci, Nagihan +6 more
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Functional metaproteomics for enzyme discovery
Discovery of microbial biocatalysts traditionally relied on activity screening of isolated bacterial strains. However, since most microorganisms cannot be cultivated in the lab, such an approach leaves the majority of the microbial enzyme diversity untapped.Marina, Prisacar, Lars I, Leichert
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Characterization of Metaproteomics in Crop Rhizospheric Soil
Journal of Proteome Research, 2010Soil rhizospheric metaproteomics is a powerful scientific tool to uncover the interactions between plants and microorganisms in the soil ecosystem. The present study established an extraction method suitable for different soils that could increase the extracted protein content.
Hai-Bin, Wang +15 more
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