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Prediction and Consequences of Cofragmentation in Metaproteomics

Journal of Proteome Research, 2019
Metaproteomics can provide critical information about biological systems, but peptides are found within a complex background of other peptides. This complex background can change across samples, in some cases drastically. Cofragmentation, the coelution of peptides with similar mass to charge ratios, is one factor that influences which peptides are ...
J. Scott P. McCain, Erin M. Bertrand
openaire   +2 more sources

The Unipept metaproteomics analysis pipeline

PROTEOMICS, 2015
Unipept ( http://unipept.ugent.be ) is a web application that offers a user‐friendly way to explore the biodiversity of complex metaproteome samples by providing interactive visualizations. In this article, the updates and changes to Unipept since its initial release are presented.
Bart, Mesuere   +5 more
openaire   +2 more sources

Perspective and Guidelines for Metaproteomics in Microbiome Studies

Journal of Proteome Research, 2019
The microbiome is emerging as a prominent factor affecting human health, and its dysbiosis is associated with various diseases. Compositional profiling of microbiome is increasingly being supplemented with functional characterization. Metaproteomics is intrinsically focused on functional changes and therefore will be an important tool in those studies ...
Xu Zhang, Daniel Figeys, Xu Zhang
exaly   +3 more sources

Interactive Chord Visualization for Metaproteomics

2017 28th International Workshop on Database and Expert Systems Applications (DEXA), 2017
Metaproteomics is an analytic approach to research microorganisms that live in complex microbial communities. A key aspect of understanding microbial communities is to link the functions of proteins identified by metaproteomics to their taxonomy. In this paper we demonstrate the interactive chord visualization as a powerful tool to explore such data ...
Roman Zoun   +5 more
openaire   +1 more source

Metaproteomics Analysis of Host–Microbiota Interfaces

2021
Metaproteomics of host-microbiome interfaces comprises the analysis of complex mixtures of bacteria, archaea, fungi, and viruses in combination with its host cells. Microbial niches can be found all over the host including the skin, oral cavity, and the intestine and are considered to be essential for the homeostasis.
Sjoerd, van der Post, Liisa, Arike
openaire   +2 more sources

Data-independent acquisition in metaproteomics

Expert Review of Proteomics
Metaproteomics offers insights into the function of complex microbial communities, while it is also capable of revealing microbe-microbe and host-microbe interactions. Data-independent acquisition (DIA) mass spectrometry is an emerging technology, which holds great potential to achieve deep and accurate metaproteomics with higher reproducibility yet ...
Enhui Wu   +3 more
openaire   +2 more sources

Metaproteomics Study of the Gut Microbiome

2018
Proteomics is a widely used method for defining the protein composition of a complex sample. As this approach allows for identification and quantification of proteins across a broad dynamic range as well as detection of post-translational modifications, proteomics is an ideal platform to investigate the gut microbiome at a functional level.
Lisa A, Lai   +3 more
openaire   +2 more sources

Metaproteome and metabolome of oral microbial communities

Periodontology 2000, 2020
AbstractThe emergence of high‐throughput technologies for the comprehensive measurement of biomolecules, also referred to as “omics” technologies, has helped us gather “big data” and characterize microbial communities. In this article, we focus on metaproteomic and metabolomic approaches that support hypothesis‐driven investigations on various oral ...
Bostanci, Nagihan   +6 more
openaire   +2 more sources

Functional metaproteomics for enzyme discovery

Discovery of microbial biocatalysts traditionally relied on activity screening of isolated bacterial strains. However, since most microorganisms cannot be cultivated in the lab, such an approach leaves the majority of the microbial enzyme diversity untapped.
Marina, Prisacar, Lars I, Leichert
openaire   +2 more sources

Characterization of Metaproteomics in Crop Rhizospheric Soil

Journal of Proteome Research, 2010
Soil rhizospheric metaproteomics is a powerful scientific tool to uncover the interactions between plants and microorganisms in the soil ecosystem. The present study established an extraction method suitable for different soils that could increase the extracted protein content.
Hai-Bin, Wang   +15 more
openaire   +2 more sources

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