Reconstructing the diversification of subtilisins in the pathogenic fungus Metarhizium anisopliae
Gene, 2004Fungi secrete subtilisin proteinases to acquire nutrients and breach host barriers. Here we sought a global characterization of the diversity of subtilisins in the insect pathogen Metarhizium anisopliae. Expressed sequence tag (EST) analyses showed that a broad host range strain of M. anisopliae sf.
Savita, Bagga +3 more
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The Metarhizium anisopliae trp1 Gene: Cloning and Regulatory Analysis
Current Microbiology, 2004The trp1 gene from the entomopathogenic fungus Metarhizium anisopliae, cloned by heterologous hybridization with the plasmid carrying the trpC gene from Aspergillus nidulans, was sequence characterized. The predicted translation product has the conserved catalytic domains of glutamine amidotransferase (G domain), indoleglycerolphosphate synthase (C ...
Charley Christian, Staats +4 more
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Investigations on the destruxin production of the entomopathogenic fungus Metarhizium anisopliae
Journal of Invertebrate Pathology, 2004The dynamics of cyclic peptide destruxins (dtxs) produced by Metarhizium anisopliae strains V245 and V275 were monitored both on solid and in liquid media. The results showed that both strains did not produce dtxs in large-scale fermenter cultures or solid Czapek Dox (CD) agar.
Chengshu, Wang +2 more
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Production of Cyclodepsipeptides Destruxin A and B from Metarhizium anisopliae
Biotechnology Progress, 2000Maltose and peptone were the best carbon and nitrogen sources for the production of destruxins from Metarhizium anisopliae. With the addition of 0.1% (w/v) beta-alanine to the basal medium, the yields of cyclodepsipeptides DA and DB were 7.2 and 279 mg/L, respectively, which was 2-fold higher than that of control experiment.
B L, Liu, J W, Chen, Y M, Tzeng
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Evaluation of Spore Density in Metarhizium anisopliae
BIOINFOLET - A Quarterly Journal of Life SciencesEntomopathogenic fungi (EPF) are recognized as eco-friendly alternatives to chemical insecticides for insect pest management. Among them, Metarhizium anisopliae has shown broad-spectrum pathogenicity against various agricultural and forest pests. The efficacy of M. anisopliae largely depends on the density and viability of its conidia (asexual spores),
Rupali Salunkhe +2 more
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Hydrated conidia of Metarhizium anisopliae release a family of metalloproteases
Journal of Invertebrate Pathology, 2007Metarhizium anisopliae spores release isoforms of metalloprotease during hydration over a 4-day incubation period. The isoforms were identified and characterized by using one-dimensional native PAGE (1-DE nPAGE) and one-dimensional SDS non-dissociating (1-DE nSDS-PAGE) zymography. The ability of these isozymes to degrade gelatin varied as revealed by 2-
Sohail S, Qazi, George G, Khachatourians
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Circular dichroism analysis of destruxins from Metarhizium anisopliae
Journal of Biochemical and Biophysical Methods, 2005Destruxins are secondary metabolites secreted by Metarhizium anisopliae [Y. Kodaira, Toxic substances to insects, produced by Aspergillus ochraceus and Oopsra destructor, Agric. Biol. Chem., 25 (1961) 261-262. D.W. Roberts, Toxins from the entomogenous fungus Metarhizium anisoplaie: Isolation from submerged cultures, J. Invertebr. Pathol., 14 (1969) 82-
Kou-Cheng, Peng +3 more
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A Strain of the Fungus Metarhizium anisopliae for Controlling Subterranean Termites
Journal of Economic Entomology, 2005Alates of the Formosan subterranean termite, Coptotermes formosanus Shiraki, collected after swarming in 2002 died within 48 h, and the cadavers were visibly infected with a fungus. Fungi were picked from the cadavers, transferred to media, and ultimately isolated to purity.
Maureen S, Wright +2 more
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Whey for mass production of Beauveria bassiana and Metarhizium anisopliae
Mycological Research, 2008Spore production of Beauveria bassiana and Metarhizium anisopliae was studied in a novel whey-based culture media. Spore yield and viability were determined for two B. bassiana (GHA-726 and CA-603) and two M. anisopliae (CA-1 and IMI 330189) isolates following production in three whey-based systems: solid, liquid, and a diphasic production system.
Adane, Kassa +8 more
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Metarhizium anisopliae: An Overview as Entomopathogene Pathogenicity
JOURNAL OF ENVIRONMENT AND BIO-SCIENCEEvery year, it is observed that certain arthropods, including Diptera, Hemiptera, Coleoptera, and Lepidoptera, have a detrimental influence to crop. A major shift in the climate leads to a rise in insect pest attacks on a variety of crops. Synthetic chemical fertilizers are frequently employed as herbicides and insecticides to control crop loss.
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