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Reconstructing the diversification of subtilisins in the pathogenic fungus Metarhizium anisopliae

Gene, 2004
Fungi secrete subtilisin proteinases to acquire nutrients and breach host barriers. Here we sought a global characterization of the diversity of subtilisins in the insect pathogen Metarhizium anisopliae. Expressed sequence tag (EST) analyses showed that a broad host range strain of M. anisopliae sf.
Savita, Bagga   +3 more
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The Metarhizium anisopliae trp1 Gene: Cloning and Regulatory Analysis

Current Microbiology, 2004
The trp1 gene from the entomopathogenic fungus Metarhizium anisopliae, cloned by heterologous hybridization with the plasmid carrying the trpC gene from Aspergillus nidulans, was sequence characterized. The predicted translation product has the conserved catalytic domains of glutamine amidotransferase (G domain), indoleglycerolphosphate synthase (C ...
Charley Christian, Staats   +4 more
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Investigations on the destruxin production of the entomopathogenic fungus Metarhizium anisopliae

Journal of Invertebrate Pathology, 2004
The dynamics of cyclic peptide destruxins (dtxs) produced by Metarhizium anisopliae strains V245 and V275 were monitored both on solid and in liquid media. The results showed that both strains did not produce dtxs in large-scale fermenter cultures or solid Czapek Dox (CD) agar.
Chengshu, Wang   +2 more
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Production of Cyclodepsipeptides Destruxin A and B from Metarhizium anisopliae

Biotechnology Progress, 2000
Maltose and peptone were the best carbon and nitrogen sources for the production of destruxins from Metarhizium anisopliae. With the addition of 0.1% (w/v) beta-alanine to the basal medium, the yields of cyclodepsipeptides DA and DB were 7.2 and 279 mg/L, respectively, which was 2-fold higher than that of control experiment.
B L, Liu, J W, Chen, Y M, Tzeng
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Evaluation of Spore Density in Metarhizium anisopliae

BIOINFOLET - A Quarterly Journal of Life Sciences
Entomopathogenic fungi (EPF) are recognized as eco-friendly alternatives to chemical insecticides for insect pest management. Among them, Metarhizium anisopliae has shown broad-spectrum pathogenicity against various agricultural and forest pests. The efficacy of M. anisopliae largely depends on the density and viability of its conidia (asexual spores),
Rupali Salunkhe   +2 more
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Hydrated conidia of Metarhizium anisopliae release a family of metalloproteases

Journal of Invertebrate Pathology, 2007
Metarhizium anisopliae spores release isoforms of metalloprotease during hydration over a 4-day incubation period. The isoforms were identified and characterized by using one-dimensional native PAGE (1-DE nPAGE) and one-dimensional SDS non-dissociating (1-DE nSDS-PAGE) zymography. The ability of these isozymes to degrade gelatin varied as revealed by 2-
Sohail S, Qazi, George G, Khachatourians
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Circular dichroism analysis of destruxins from Metarhizium anisopliae

Journal of Biochemical and Biophysical Methods, 2005
Destruxins are secondary metabolites secreted by Metarhizium anisopliae [Y. Kodaira, Toxic substances to insects, produced by Aspergillus ochraceus and Oopsra destructor, Agric. Biol. Chem., 25 (1961) 261-262. D.W. Roberts, Toxins from the entomogenous fungus Metarhizium anisoplaie: Isolation from submerged cultures, J. Invertebr. Pathol., 14 (1969) 82-
Kou-Cheng, Peng   +3 more
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A Strain of the Fungus Metarhizium anisopliae for Controlling Subterranean Termites

Journal of Economic Entomology, 2005
Alates of the Formosan subterranean termite, Coptotermes formosanus Shiraki, collected after swarming in 2002 died within 48 h, and the cadavers were visibly infected with a fungus. Fungi were picked from the cadavers, transferred to media, and ultimately isolated to purity.
Maureen S, Wright   +2 more
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Whey for mass production of Beauveria bassiana and Metarhizium anisopliae

Mycological Research, 2008
Spore production of Beauveria bassiana and Metarhizium anisopliae was studied in a novel whey-based culture media. Spore yield and viability were determined for two B. bassiana (GHA-726 and CA-603) and two M. anisopliae (CA-1 and IMI 330189) isolates following production in three whey-based systems: solid, liquid, and a diphasic production system.
Adane, Kassa   +8 more
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Metarhizium anisopliae: An Overview as Entomopathogene Pathogenicity

JOURNAL OF ENVIRONMENT AND BIO-SCIENCE
Every year, it is observed that certain arthropods, including Diptera, Hemiptera, Coleoptera, and Lepidoptera, have a detrimental influence to crop. A major shift in the climate leads to a rise in insect pest attacks on a variety of crops. Synthetic chemical fertilizers are frequently employed as herbicides and insecticides to control crop loss.
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