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Metatranscriptomics: A Tool for Clinical Metagenomics
OMICS: A Journal of Integrative BiologyIn the field of bioinformatics, amplicon sequencing of 16S rRNA genes has long been used to investigate community membership and taxonomic abundance in microbiome studies. As we can observe, shotgun metagenomics has become the dominant method in this field.
Shivani Tyagi, Pramod Katara
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Metatranscriptome and Resistome of the Endodontic Microbiome
Journal of EndodonticsINTRODUCTION In this study, we used metatranscriptomics for the first time to investigate microbial composition, functional signatures, and antimicrobial resistance gene expression in endodontic infections. METHODS Root canal samples were collected from ten teeth, including five primary and five persistent/secondary endodontic infections.
Pinheiro, Ericka T +9 more
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Gene Expression Profiling: Metatranscriptomics
2011Metatranscriptomics has been developed to help understand how communities respond to changes in their environment. Metagenomic studies provided a snapshot of the genetic composition of the community at any given time. However, short-timescale studies investigating the response of communities to rapid environmental changes (e.g.
Jack A, Gilbert, Margaret, Hughes
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Usage of Metatranscriptomics to Understand Oral Disease
2021Metatranscriptomics is a method used to comprehensively capture bacterial activity within microbiota at the transcription level. It has become an alternative to the 16S rDNA sequencing, which uses only the 16S rRNA gene for predicting bacterial composition.
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Advances and Challenges in Metatranscriptomic Analysis
2021New-era genomic tools have proved themselves a big boon in showing a path to get an insight to microbial evolution, taxonomic profiling, active members of a community and the genes involved in many metabolic pathways. Shotgun metagenomics helps in random sequencing of genome of the studied microbiome; however, it does not show the exact number of ...
Anushka Singh +2 more
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A Hitchhiker’s Guide to Metatranscriptomics
2016Recent technological development in high throughput DNA sequencing has opened many venues for biological systems analysis, where the data generation is no longer the bottleneck but its analysis. The sequencing technological advancements are being used in the study of a large and previously unknown microbe world; this unknown world has revealed itself ...
Mariana Peimbert, Luis David Alcaraz
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Enhancing Arbovirus Surveillance with Metatranscriptomics
2021Submitted in total fulfilment of the requirements for the degree of Doctor of Philosophy to the School of Applied Systems Biology, College of Science, Health and Engineering, La Trobe University, Victoria.This thesis was a recipient of the Nancy Millis Award for theses of exceptional merit.
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Metatranscriptomics catches gut microbes in the act
Cell Host & MicrobeIn this issue of Cell Host & Microbe, Flores Ramos et al.1 employ metatranscriptomics to uncover diurnal microbial functional shifts in the gut microbiome driven by time-restricted feeding. Their work highlights the value of metatranscriptomics over metagenomics in capturing real-time microbial activity and guiding therapeutic bacterial engineering.
Kailin, Liu, Yuhao, Wang
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Generation and Analysis of Microbial Metatranscriptomes
2015Analysis of the collective RNA pool from a microbial community - the metatranscriptome - yields valuable information on microbial gene expression patterns and biogeochemical processes in natural environments. Molecular and analytical tools for analyzing metatranscriptomes using high-throughput sequencing have advanced rapidly in recent years and ...
Neha Sarode +4 more
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