Results 251 to 260 of about 18,529 (279)

Metatranscriptomics and metabarcoding reveal spatiotemporal shifts in fungal communities and their activities in Chinese coastal waters

Molecular Ecology, 2023
Fungal communities are diverse and abundant in coastal waters, yet, their ecological roles and adaptations remain largely unknown. To address these gaps, ITS2 metabarcoding and metatranscriptomic analyses were used to capture the whole suite of fungal ...
Mengmeng Wang, P. Mara, G. Burgaud, V. Edgcomb, Xuedan Long, Haiping Yang, L. Cai, Wei Li   +7 more
semanticscholar   +1 more source

Metatranscriptomics for the Human Microbiome and Microbial Community Functional Profiling.

Annual Review of Biomedical Data Science, 2021
Shotgun metatranscriptomics (MTX) is an increasingly practical way to survey microbial community gene function and regulation at scale. This review begins by summarizing the motivations for community transcriptomics and the history of the field.
Yancong Zhang, Kelsey N. Thompson, Tobyn A. Branck, Yan Yan, L. Nguyen, E. Franzosa, C. Huttenhower   +6 more
semanticscholar   +1 more source

Deciphering Metatranscriptomic Data

2014
Metatranscriptomic data contributes another piece of the puzzle to understanding the phylogenetic structure and function of a community of organisms. High-quality total RNA is a bountiful mixture of ribosomal, transfer, messenger and other noncoding RNAs, where each family of RNA is vital to answering questions concerning the hidden microbial world ...
Kopylova, Evguenia, Noé, Laurent, da Silva, Corinne, Berthelot, Jean-Frédéric, Alberti, Adriana, Aury, Jean-Marc, Touzet, Helene   +6 more
openaire   +4 more sources

Quantitative Microbial Metatranscriptomics

2014
The direct retrieval and sequencing of environmental RNA is emerging as a powerful technique to elucidate the in situ activities of microbial communities. Here we provide a metatranscriptomic protocol describing environmental sample collection, rRNA depletion, mRNA amplification, cDNA synthesis, and bioinformatic analysis.
Scott, Gifford, Brandon, Satinsky, Mary Ann, Moran   +2 more
openaire   +2 more sources

Gene Expression Profiling: Metatranscriptomics

2011
Metatranscriptomics has been developed to help understand how communities respond to changes in their environment. Metagenomic studies provided a snapshot of the genetic composition of the community at any given time. However, short-timescale studies investigating the response of communities to rapid environmental changes (e.g.
Jack A, Gilbert, Margaret, Hughes
openaire   +2 more sources

Genome-Resolved Metagenomics and Metatranscriptomics Reveal that Aquificae Dominates Arsenate Reduction in Tengchong Geothermal Springs.

Environmental Science and Technology, 2022
Elevated arsenic (As) is common in geothermal springs, shaping the evolution of As metabolism genes and As transforming microbes. Herein, genome-level microbial metabolisms and As cycling strategies in Tengchong geothermal springs were demonstrated for ...
Zhipeng Yin, L. Ye, C. Jing
semanticscholar   +1 more source

Metatranscriptomics of Soil Eukaryotic Communities

2016
Functions expressed by eukaryotic organisms in soil can be specifically studied by analyzing the pool of eukaryotic-specific polyadenylated mRNA directly extracted from environmental samples. In this chapter, we describe two alternative protocols for the extraction of high-quality RNA from soil samples.
Yadav, Rajiv, Bragalini, Claudia, Fraissinet-Tachet, Laurence, Marmeisse, Roland, Luis, Patricia   +4 more
openaire   +3 more sources

Application of metatranscriptomics to soil environments

Journal of Microbiological Methods, 2012
The activities of soil microbial communities are of critical importance to terrestrial ecosystem functioning. The mechanisms that determine the interactions between soil microorganisms, their environment and neighbouring organisms, however, are poorly understood.
Carvalhais, Lilia C., Dennis, Paul G., Tyson, Gene W., Schenk, Peer M.   +3 more
openaire   +3 more sources

Metagenomics and Metatranscriptomics

2022
International ...
Guyomar, Cervin, Lemaitre, Claire
openaire   +1 more source

Metatranscriptomic analyses of the oral microbiome

Periodontology 2000, 2020
AbstractAlthough the composition of the oral human microbiome is now well studied, regulation of genes within oral microbial communities remains mostly uncharacterized. Current concepts of periodontal disease and caries highlight the importance of oral biofilms and their role as etiological agents of those diseases.
openaire   +2 more sources