Results 31 to 40 of about 481 (139)

Evolutionary cell biology: Functional insight from “Endless forms most beautiful” [PDF]

open access: yes, 2015
In animal and fungal model organisms, the complexities of cell biology have been analyzed in exquisite detail and much is known about how these organisms function at the cellular level.
Dacks, Joel B.   +4 more
core   +1 more source

Nitrous oxide and methane in the Atlantic Ocean between 50 degrees North and 52 degrees South: Latitudinal distribution and sea-to-air flux [PDF]

open access: yes, 2008
We discuss nitrous oxide (N2O) and methane (CH4) distributions in 49 vertical profiles covering the upper 300 m of the water column along two 13,500 km transects between 50°N and 52°S during the Atlantic Meridional Transect (AMT) programme (AMT cruises ...
Forster, Grant   +5 more
core   +1 more source

Diversity of the DNA Replication System in the Archaea Domain

open access: yesArchaea, Volume 2014, Issue 1, 2014., 2014
The precise and timely duplication of the genome is essential for cellular life. It is achieved by DNA replication, a complex process that is conserved among the three domains of life. Even though the cellular structure of archaea closely resembles that of bacteria, the information processing machinery of archaea is evolutionarily more closely related ...
Felipe Sarmiento   +4 more
wiley   +1 more source

Development of β‐Lactamase as a Tool for Monitoring Conditional Gene Expression by a Tetracycline‐Riboswitch in Methanosarcina acetivorans

open access: yesArchaea, Volume 2014, Issue 1, 2014., 2014
The use of reporter gene fusions to assess cellular processes such as protein targeting and regulation of transcription or translation is established technology in archaeal, bacterial, and eukaryal genetics. Fluorescent proteins or enzymes resulting in chromogenic substrate turnover, like β‐galactosidase, have been particularly useful for microscopic ...
Shemsi Demolli   +6 more
wiley   +1 more source

Genetic Confirmation of the Role of Sulfopyruvate Decarboxylase in Coenzyme M Biosynthesis in Methanococcus maripaludis

open access: yesArchaea, Volume 2013, Issue 1, 2013., 2013
Coenzyme M is an essential coenzyme for methanogenesis. The proposed biosynthetic pathway consists of five steps, of which the fourth step is catalyzed by sulfopyruvate decarboxylase (ComDE). Disruption of the gene comE by transposon mutagenesis resulted in a partial coenzyme M auxotroph, which grew poorly in the absence of coenzyme M and retained less
Felipe Sarmiento   +3 more
wiley   +1 more source

Comparative genomics highlights the unique biology of Methanomassiliicoccales, a Thermoplasmatales-related seventh order of methanogenic archaea that encodes pyrrolysine [PDF]

open access: yes, 2014
BACKGROUND: A seventh order of methanogens, the Methanomassiliicoccales, has been identified in diverse anaerobic environments including the gastrointestinal tracts (GIT) of humans and other animals and may contribute significantly to methane emission ...
Bardot, Olivier   +11 more
core   +4 more sources

Establishing a Markerless Genetic Exchange System for Methanosarcina mazei Strain Gö1 for Constructing Chromosomal Mutants of Small RNA Genes

open access: yesArchaea, Volume 2011, Issue 1, 2011., 2011
A markerless genetic exchange system was successfully established in Methanosarcina mazei strain Gö1 using the hpt gene coding for hypoxanthine phosphoribosyltransferase. First, a chromosomal deletion mutant of the hpt gene was generated conferring resistance to the purine analog 8‐aza‐2,6‐diaminopurine (8‐ADP).
Claudia Ehlers   +3 more
wiley   +1 more source

Toward a List of Molecules as Potential Biosignature Gases for the Search for Life on Exoplanets and Applications to Terrestrial Biochemistry [PDF]

open access: yes, 2016
Thousands of exoplanets are known to orbit nearby stars. Plans for the next generation of space-based and ground-based telescopes are fueling the anticipation that a precious few habitable planets can be identified in the coming decade.
Bains, William   +2 more
core   +1 more source

S‐Layer Glycoproteins and Flagellins: Reporters of Archaeal Posttranslational Modifications

open access: yesArchaea, Volume 2010, Issue 1, 2010., 2010
Many archaeal proteins undergo posttranslational modifications. S‐layer proteins and flagellins have been used successfully to study a variety of these modifications, including N‐linked glycosylation, signal peptide removal and lipid modification. Use of these well‐characterized reporter proteins in the genetically tractable model organisms, Haloferax ...
Ken F. Jarrell   +5 more
wiley   +1 more source

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