Results 191 to 200 of about 6,262,928 (308)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

VI European Congress of Methodology [PDF]

, 2015
Methodology and statistics for the behavioural and social sciences, van de Schoot, A.G.J., Moerbeek, M., Leerstoel Hoijtink, Leerstoel Heijden   +4 more
core   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Disease Burden of Type 2 Diabetes Among Young Adults in Asia: An Analysis From the Global Burden of Disease Study 2021

Journal of Diabetes Research
Ruoting Wang, Gregory Y. H. Lip, Yingxin Liu, Ningyu Qi, Xuerui Bai, Lehana Thabane, Guowei Li, Harriette G. C. Van Spall   +7 more
doaj   +1 more source

Current Gaps in Point-of-Care Ultrasound Supervision in Generalist Practice in Japan: A Nationwide Survey. [PDF]

J Gen Fam Med
Yamada T, Kimura T, Shigetomi K, Shinohara T, Ouchi S, Mabuchi S, Ichige H, Kameda T, Yoshino S, Kitano Y, Endo K, Okada K, Kamijo H, Takahashi H, Kusama T, Ishida T, Hashimoto M.   +16 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Dendritic shaft constrictions shape synaptic integration in neurons. [PDF]

Sci Adv
Kelly T, Döngi M, Rodriguez-Gatica JE, Ofer N, Wert-Carvajal C, Cissewski N, Barboni M, Bethge P, Godale CM, Yaser S, Herde MK, Tillmann J, Peter SI, Dupraz S, Koch H, Stein V, Bradke F, Danzer SC, Tchumatchenko T, Schwarz MK, Kubitscheck U, Nägerl UV, Beck H.   +22 more
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Contextualizing the ASPE standards of best practice: reflecting on the past, present, and future of SP methodology. [PDF]

Adv Simul (Lond)
Wallace AM, Krebsbach C, Radway J, Clark L.   +3 more
europepmc   +1 more source

Using cell‐free RNA to identify B‐ and T‐cell clonality for diagnosis and monitoring of B‐ and T‐cell neoplasms

FEBS Open Bio, EarlyView.
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar, Sally Agersborg, Ahmed Charifa, Pooja Phull, Noa Biran, David Vesole, Harsh Parmar, Andrew Pecora, Andrew Ip, Andre Goy, David Siegel, Maher Albitar   +11 more
wiley   +1 more source