Results 171 to 180 of about 9,958,067 (302)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Correction to "The Role of Dopamine Signaling and Neuroinflammation in Age-Related Cognitive Dysfunction". [PDF]

Brain Behav
europepmc   +1 more source

Clonal hematopoiesis dynamics influences long-term outcomes of follicular lymphoma: Results from FIL FOLL12 trial. [PDF]

Hemasphere
Maher N, Moia R, Almasri M, Cividini L, Genuardi E, Cosentino C, Soscia R, Assanto GM, Tavarozzi R, Vegliante MC, Lorenzi L, Andorno A, Arcaini L, Ragaini S, Puccini B, Patti C, Santoro A, Margiotta-Casaluci G, Zilioli VR, Zanni M, Ronconi S, Di Raimondo F, Arcari A, Califano C, Castellino C, Conconi A, Perrone T, Mannina D, Plenteda C, Alesiani F, Rossi FG, Carella AM, Marcheselli L, Galimberti S, Ciavarella S, Bomben R, Del Giudice I, Ladetto M, Gaidano G, Luminari S, Ferrero S.   +40 more
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Correction to "Scabies Outbreak in Pediatric Populations of Bangladesh: A Perspective on Therapeutic Management, Risk Factors, and Public Health Implications". [PDF]

Health Sci Rep
europepmc   +1 more source

The Modified Spinal Instability Spondylodiscitis Score (mSISS): Adaptation and Validation of a Novel Classification System for Spinal Instability in Spondylodiscitis. [PDF]

Global Spine J
Schömig F, Taheri N, El-Sharkawi M, Aly MM, Yurac R, Camino Willhuber G, Dandurand C, Holas M, Chhabra HS, Schnake K, Reinhold M, Joaquim AF, Schroeder GD, Thomé C, Scherer J, Popescu EC, Spiegl UJA, Bigdon SF, Bransford R, Pumberger M.   +19 more
europepmc   +1 more source

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

FEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell, Cassy Ross, Panagiota S. Filippou, Bruno Silvester Lopes, Dominic Gilchrist, Paul Dean, Kamar Ameen‐Ali, Tora K. Smulders‐Srinivasan, Ahmad A. Khundakar   +8 more
wiley   +1 more source

Dental Students' Perceptions of a Self-Directed Simulation-Based Learning Methodology (MAES©): A Pilot Study. [PDF]

Dent J (Basel)
Guzmán S, García A, Velló-Ribes MÁ, Cortés O.   +3 more
europepmc   +1 more source