Results 151 to 160 of about 2,951,798 (263)

Case Report: E-cadherin and Vimentin expression in advanced-stage pancreatic ductal adenocarcinoma tissue and circulating tumor cells: preliminary results from a pilot study. [PDF]

Front Oncol
Rapanotti MC, Scioli MG, Moscardelli A, Troncone E, Cugini E, Viguria TMS, Vergilii L, Puzzuoli M, Anzillotti S, Terriaca S, Cenci T, Muzzi E, De Cristofaro E, Savino L, Toti L, De Luca A, Ferlosio A, Del Vecchio Blanco G, Orlandi A.   +18 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Migratory Bird and Marine Mammal Surveillance Fails to Find Evidence for an HPAI H5N1 2.3.4.4b Incursion Into Australia in 2025. [PDF]

Influenza Other Respir Viruses
Wille M, Ross TA, Atkinson R, Boyle DB, Christie M, Dewar ML, Douglas TK, Gray R, Hansen B, Jessop R, Kidd LR, Marks I, Mileto P, Miller E, Neave MJ, Ryding S, Sutherland DR, Yu H, Klaassen M.   +18 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

From Fly on the Wall to Future Colleagues: Best Practice Recommendations for Medical Student Shadowing Programs. [PDF]

AEM Educ Train
Estes M, Matonis D, Knych M, Barbas B, Ren R, Dixon W, Pasirstein M, Zhang XC, Bowers K, Chauhan RR, Patti LA.   +10 more
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Correction to "Scabies Outbreak in Pediatric Populations of Bangladesh: A Perspective on Therapeutic Management, Risk Factors, and Public Health Implications". [PDF]

Health Sci Rep
europepmc   +1 more source

Plasma cytokine profiles in breast cancer patients and their association with therapeutic response in Peru: a prospective cohort study. [PDF]

Front Immunol
Vela-Ruiz JM, Morante Z, Ferreyra Y, Galvez-Villanueva MA, Valencia F, Campos-Tineo JJ, Callapiña De Paz M, Córdova-Salazar AA, Marcos-Carbajal P, Moreno Lujan JM, Pantoja Lazaro AR, Escobar Caipo LG, Flores Trujillo GA, Cusma Quintana TN, De La Cruz-Vargas JA, Gomez HL, Soto A.   +16 more
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source