Results 161 to 170 of about 6,262,928 (308)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Advancing relational methods in agri-food governance research and beyond: Introducing the Socio-Material Actor-Network Analysis (SMANA) methodology. [PDF]

Open Res Eur
Zerbian T, Furlan V, López-García D.
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

See better, treat better - cap-assisted single-operator pancreatoscopy for electrohydraulic lithotripsy of pancreatic duct stones. [PDF]

Endoscopy
Ellrichmann M, Schäfer N, Halfmann F, Hamel H, Fehrendt H, Meinhardt C.   +5 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Evaluation of Multi-Stakeholder Perspectives on the Preparedness of Ghana's Animal Health Services for African Swine Fever Control: A Cross-Sectional Study. [PDF]

Health Sci Rep
Allege-Cudjoe E, Emikpe BO, Ayamdooh EN, Abudu M, Acquah HNB, Yakubu AR, Yeboah S, Takyi PN, Abuh JK, Mensah PA, Eshun E, Doe F, Ababio P, Kwoba EN, Onidje E, Meyir ZY, Tasiame W, Atawalna J, Ahmed GM, Boka MO, Wolde A, Akunzule A, Mensah C, Fenteng ED.   +23 more
europepmc   +1 more source

Methodological Flaws [PDF]

Deutsches Ärzteblatt international, 2013
Schmiemann, Guido, Gagyor, Ildiko, Hummers-Pradier, Eva, Bleidorn, Jutta   +3 more
openaire   +3 more sources

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Perioperative antithrombotic management in degenerative spine surgery: a multicenter cohort of 767 consecutive patients. [PDF]

Front Med (Lausanne)
Corazzelli G, Corvino S, Rios DL, Gentile D, Di Domenico C, Corazzelli F, De Rosa G, Fiore F, Germano C, Filippelli M, Colella N, Ricciardi F, D'Elia A, Leonetti S, Sacco M, Meglio V, Pizzuti V, Di Russo P, Gorgoglione N, Petrella G, Mastantuoni C, Scala MR, de Angelis M, De Bellis A, Cioffi V, Aloj F, Sigona L, Bocchetti A, Di Colandrea S, Coscia G, Passavanti MB, Cuomo A, De Marinis P, Tocchetti CG, de Divitiis O, Mormile M, Cirillo P, Catapano G, de Falco R, Paolini S, Esposito V, Innocenzi G.   +41 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source