Results 21 to 30 of about 516,288 (261)

Effects of lysate/tissue storage at −80°C on subsequently extracted EVs of epithelial ovarian cancer tissue origins

iScience, 2023
Summary: Small extracellular vesicles (sEVs) and large extracellular vesicles (lEVs), play vital roles in intercellular communication. We optimized a method that extracts EVs from epithelial ovarian cancer (EOC) tissues for the purpose of investigating ...
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng   +7 more
doaj   +1 more source

A PEROXO-Tag Enables Rapid Isolation of Peroxisomes from Human Cells

iScience, 2020
Summary: Peroxisomes are metabolic organelles that perform a diverse array of critical functions in human physiology. Traditional isolation methods for peroxisomes can take more than 1 h to complete and can be laborious to implement.
G. Jordan Ray, Elizabeth A. Boydston, Emily Shortt, Gregory A. Wyant, Sebastian Lourido, Walter W. Chen, David M. Sabatini   +6 more
doaj   +1 more source

Behavioral variation across the days and lives of honey bees

iScience, 2022
Summary: In honey bee colonies, workers generally change tasks with age (from brood care, to nest work, to foraging). While these trends are well established, our understanding of how individuals distribute tasks during a day, and how individuals differ ...
Michael L. Smith, Jacob D. Davidson, Benjamin Wild, David M. Dormagen, Tim Landgraf, Iain D. Couzin   +5 more
doaj   +1 more source

A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia

iScience, 2021
Summary: Organoids mimic the physiologic and pathologic events of organs. However, no consensus on esophageal organoid (EO) culture methods has been reached.
Biyun Zheng, Kyung-Pil Ko, Xuefen Fang, Xiaozhong Wang, Jie Zhang, Sohee Jun, Bong-Jun Kim, Wenyi Luo, Moon Jong Kim, Youn-Sang Jung, Christopher L. Cervantes, Jae-Il Park   +11 more
doaj   +1 more source

PCR-based profiling of transcription factor activity in vivo by a virus-based reporter battery

iScience, 2022
Summary: Understanding the molecular mechanisms of gene regulation is pivotal for understanding how cells establish and modify their identities and functions.
Hitomi Abe, Kentaro Abe
doaj   +1 more source

Integrating extrusion complex-associated pattern to predict cell type-specific long-range chromatin loops

iScience, 2022
Summary: The chromatin loop plays a critical role in the study of gene expression and disease. Supervised learning-based algorithms to predict the chromatin loops require large priori information to satisfy the model construction, while the prediction ...
Yajing Deng, Li Tang, Xiaolong Zhou, Wenkang Wang, Min Li   +4 more
doaj   +1 more source

A human multi-cellular model shows how platelets drive production of diseased extracellular matrix and tissue invasion

iScience, 2021
Summary: Guided by a multi-level “deconstruction” of omental metastases, we developed a tetra (four cell)-culture model of primary human mesothelial cells, fibroblasts, adipocytes, and high-grade serous ovarian cancer (HGSOC) cell lines.
Beatrice Malacrida, Sam Nichols, Eleni Maniati, Roanne Jones, Robin Delanie-Smith, Reza Roozitalab, Eleanor J. Tyler, Morgan Thomas, Gina Boot, Jonas Mackerodt, Michelle Lockley, Martin M. Knight, Frances R. Balkwill, Oliver M.T. Pearce   +13 more
doaj   +1 more source

A quantitative micro-tomographic gut atlas of the lepidopteran model insect Manduca sexta

iScience, 2023
Summary: The tobacco hornworm is used extensively as a model system for ecotoxicology, immunology and gut physiology. Here, we established a micro-computed tomography approach based on the oral application of the clinical contrast agent iodixanol ...
Anton G. Windfelder, Jessica Steinbart, Ulrich Flögel, Jan Scherberich, Marian Kampschulte, Gabriele A. Krombach, Andreas Vilcinskas   +6 more
doaj   +1 more source

Spatio-temporal optical coherence tomography provides full thickness imaging of the chorioretinal complex

iScience, 2022
Summary: Despite the rapid development of optical imaging methods, high-resolution in vivo imaging with penetration into deeper tissue layers is still a major challenge. Optical coherence tomography (OCT) has been used successfully for non-invasive human
Egidijus Auksorius, Dawid Borycki, Piotr Wegrzyn, Bartosz L. Sikorski, Kamil Lizewski, Ieva Zickiene, Mounika Rapolu, Karolis Adomavicius, Slawomir Tomczewski, Maciej Wojtkowski   +9 more
doaj   +1 more source

GATA1 deletion in human pluripotent stem cells increases differentiation yield and maturity of neutrophils

iScience, 2023
Summary: Human pluripotent stem cell (hPSC)-derived tissues can be used to model diseases in cell types that are challenging to harvest and study at-scale, such as neutrophils.
Thomas C. Harper, Elaine M. Oberlick, Tomas J. Smith, Duncan E. Nunes, Mark-Anthony Bray, Seonmi Park, Corey D. Driscoll, Sarah F. Mowbray, Christophe Antczak   +8 more
doaj   +1 more source