Results 1 to 10 of about 1,338,720 (273)

The Rigor and Transparency Index Quality Metric for Assessing Biological and Medical Science Methods

iScience, 2020
Summary: The reproducibility crisis is a multifaceted problem involving ingrained practices within the scientific community. Fortunately, some causes are addressed by the author's adherence to rigor and reproducibility criteria, implemented via ...
Joe Menke, Martijn Roelandse, Burak Ozyurt, Maryann Martone, Anita Bandrowski   +4 more
doaj   +3 more sources

Conditions for maintenance of hepatocyte differentiation and function in 3D cultures

iScience, 2021
Summary: Spheroid cultures of primary human hepatocytes (PHH) are used in studies of hepatic drug metabolism and toxicity. The cultures are maintained under different conditions, with possible confounding results. We performed an in-depth analysis of the
Niklas Handin, Evgeniya Mickols, Magnus Ölander, Jakob Rudfeldt, Kristin Blom, Frida Nyberg, Wojciech Senkowski, Jozef Urdzik, Varun Maturi, Mårten Fryknäs, Per Artursson   +10 more
doaj   +1 more source

Top-down reconfiguration of SMA cortical connectivity during action preparation

iScience, 2023
Summary: The Bereitschaftspotential (BP), a scalp potential recorded in humans during action preparation, is characterized by a slow amplitude increase over fronto-central regions as action execution approaches.
Valentina Bianco, Eleonora Arrigoni, Francesco Di Russo, Leonor Josefina Romero Lauro, Alberto Pisoni   +4 more
doaj   +1 more source

Influence of data sampling methods on the representation of neural spiking activity in vivo

iScience, 2022
Summary: In vivo single-unit recordings distinguish the basal spiking properties of neurons in different experimental settings and disease states. Here, we examined over 300 spike trains recorded from Purkinje cells and cerebellar nuclei neurons to test ...
Meike E. van der Heijden, Amanda M. Brown, Roy V. Sillitoe   +2 more
doaj   +1 more source

Visualizing lymphocytic choriomeningitis virus infection in cells and living mice

iScience, 2022
Summary: Mammarenavirus are a large family of enveloped negative-strand RNA viruses that include several agents responsible for severe hemorrhagic fevers.
Yuxi Wen, Huan Xu, Weiwei Wan, Weijuan Shang, Runming Jin, Fen Zhou, Heng Mei, Jingshi Wang, Gengfu Xiao, Hongbo Chen, Xiaoyan Wu, Leike Zhang   +11 more
doaj   +1 more source

Optimization of the 16S rRNA sequencing analysis pipeline for studying in vitro communities of gut commensals

iScience, 2022
Summary: While microbial communities inhabit a wide variety of complex natural environments, in vitro culturing enables highly controlled conditions and high-throughput interrogation for generating mechanistic insights.
Arianna I. Celis, Andrés Aranda-Díaz, Rebecca Culver, Katherine Xue, David Relman, Handuo Shi, Kerwyn Casey Huang   +6 more
doaj   +1 more source

Cell culture systems for isolation of SARS-CoV-2 clinical isolates and generation of recombinant virus

iScience, 2023
Summary: A simple and robust cell culture system is essential for generating authentic SARS-CoV-2 stocks for evaluation of viral pathogenicity, screening of antiviral compounds, and preparation of inactivated vaccines.
Da-Yuan Chen, Jacquelyn Turcinovic, Shuchen Feng, Devin J. Kenney, Chue Vin Chin, Manish C. Choudhary, Hasahn L. Conway, Marc Semaan, Brianna J. Close, Alexander H. Tavares, Scott Seitz, Nazimuddin Khan, Sebastian Kapell, Nicholas A. Crossland, Jonathan Z. Li, Florian Douam, Susan C. Baker, John H. Connor, Mohsan Saeed   +18 more
doaj   +1 more source

Endonuclease-based genotyping of the RBM as a method to track the emergence or evolution of SARS-CoV-2 variants

iScience, 2021
Summary: Since the beginning of the COVID-19 pandemics, variants have emerged. Some of them display increased transmissibility and/or resistance to immune response.
Eva Lopez, Margot Barthélémy, Cécile Baronti, Shirley Masse, Alessandra Falchi, Fabien Durbesson, Renaud Vincentelli, Xavier de Lamballerie, Rémi Charrel, Bruno Coutard   +9 more
doaj   +1 more source

The 3D Reconstruction of Pocillopora Colony Sheds Light on the Growth Pattern of This Reef-Building Coral

iScience, 2020
Summary: Coral reefs are formed by living polyps, and understanding the dynamic processes behind the reefs is crucial for marine ecosystem restoration.
Yixin Li, Tingyu Han, Kun Bi, Kun Liang, Junyuan Chen, Jing Lu, Chunpeng He, Zuhong Lu   +7 more
doaj   +1 more source

A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells

iScience, 2022
Summary: The sinoatrial node (SAN) is the primary pacemaker of the heart. The human SAN is poorly understood due to limited primary tissue access and limitations in robust in vitro derivation methods.
Zaniar Ghazizadeh, Jiajun Zhu, Faranak Fattahi, Alice Tang, Xiaolu Sun, Sadaf Amin, Su-Yi Tsai, Mona Khalaj, Ting Zhou, Ryan M. Samuel, Tuo Zhang, Francis A. Ortega, Miriam Gordillo, Dorota Moroziewicz, Daniel Paull, Scott A. Noggle, Jenny Zhaoying Xiang, Lorenz Studer, David J. Christini, Geoffrey S. Pitt, Todd Evans, Shuibing Chen   +21 more
doaj   +1 more source