Results 21 to 30 of about 573,237 (295)

A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells

iScience, 2022
Summary: The sinoatrial node (SAN) is the primary pacemaker of the heart. The human SAN is poorly understood due to limited primary tissue access and limitations in robust in vitro derivation methods.
Zaniar Ghazizadeh, Jiajun Zhu, Faranak Fattahi, Alice Tang, Xiaolu Sun, Sadaf Amin, Su-Yi Tsai, Mona Khalaj, Ting Zhou, Ryan M. Samuel, Tuo Zhang, Francis A. Ortega, Miriam Gordillo, Dorota Moroziewicz, Daniel Paull, Scott A. Noggle, Jenny Zhaoying Xiang, Lorenz Studer, David J. Christini, Geoffrey S. Pitt, Todd Evans, Shuibing Chen   +21 more
doaj   +1 more source

Methodological studies of PIXE on biological and environmental science [PDF]

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 1996
The Van de Graaff Facility at IHEP is mainly used for PIXE research work especially on biologicial and environmental science. The sensitivities and the number of analyzed trace elements were improved greatly using special absorbers for bone and whole blood samples. Quite good results were obtained for different biomedical research work. Several methods
Sha Yin, Gu Yingmei, Liu Guodong, Wang Anpu, Zhang Peiqun, Zhu Jieqing, Shi Jian, Yang Shulan, Liu Pingsheng, Li Xiaolin   +9 more
openaire   +1 more source

Low-temperature resin embedding of the whole brain for various precise structures dissection

iScience, 2023
Summary: Resin embedding combined with ultra-thin sectioning has been widely used in microscopic and electron imaging to acquire precise structural information of biological tissues.
Jiaojiao Tian, Yingying Chen, Tao Jiang, Xueyan Jia, Hui Gong, Xiangning Li   +5 more
doaj   +1 more source

A realistic transfer method reveals low risk of SARS-CoV-2 transmission via contaminated euro coins and banknotes

iScience, 2021
Summary: The current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has created a significant threat to global health. While respiratory aerosols or droplets are considered as the main route of human-to-human transmission ...
Daniel Todt, Toni Luise Meister, Barbora Tamele, John Howes, Dajana Paulmann, Britta Becker, Florian H. Brill, Mark Wind, Jack Schijven, Natalie Heinen, Volker Kinast, Baxolele Mhlekude, Christine Goffinet, Adalbert Krawczyk, Jörg Steinmann, Stephanie Pfaender, Yannick Brüggemann, Eike Steinmann   +17 more
doaj   +1 more source

Robust induction of functional astrocytes using NGN2 expression in human pluripotent stem cells

iScience, 2023
Summary: Emerging evidence of species divergent features of astrocytes coupled with the relative inaccessibility of human brain tissue underscore the utility of human pluripotent stem cell (hPSC) technologies for the generation and study of human ...
Martin H. Berryer, Matthew Tegtmeyer, Loïc Binan, Vera Valakh, Anna Nathanson, Darina Trendafilova, Ethan Crouse, Jenny A. Klein, Daniel Meyer, Olli Pietiläinen, Francesca Rapino, Samouil L. Farhi, Lee L. Rubin, Steven A. McCarroll, Ralda Nehme, Lindy E. Barrett   +15 more
doaj   +1 more source

Antibody-based sex determination of human skeletal remains

iScience, 2023
Summary: Assignment of biological sex to skeletal remains is critical in the accurate reconstruction of the past. Analysis of sex-chromosome encoded AMELX and AMELY peptides from the enamel protein amelogenin underpins a minimally destructive mass ...
Barry Shaw, Sophie Foggin, Petter Hamilton-Stanley, Andy Barlow, Catriona Pickard, Linda Fibiger, Neil Oldham, Patrick Tighe, Lisette M. Kootker, Sarah Schrader, Rob Layfield   +10 more
doaj   +1 more source

Maximum tree lifespans derived from public-domain dendrochronological data

iScience, 2023
Summary: The public-domain International Tree-Ring Data Bank (ITRDB) is an under-utilized dataset to improve existing estimates of global tree longevity.
Franco Biondi, David M. Meko, Gianluca Piovesan   +2 more
doaj   +1 more source

Visualization of PS/γ-Secretase Activity in Living Cells

iScience, 2020
Summary: A change in Presenilin (PS)/γ-secretase activity is linked to essential biological events as well as to the progression of many diseases. However, not much is known about how PS/γ-secretase activity is spatiotemporally regulated in cells. One of
Masato Maesako, Nicole M. Sekula, Anna Aristarkhova, Polina Feschenko, Lauren C. Anderson, Oksana Berezovska   +5 more
doaj   +1 more source

Effects of lysate/tissue storage at −80°C on subsequently extracted EVs of epithelial ovarian cancer tissue origins

iScience, 2023
Summary: Small extracellular vesicles (sEVs) and large extracellular vesicles (lEVs), play vital roles in intercellular communication. We optimized a method that extracts EVs from epithelial ovarian cancer (EOC) tissues for the purpose of investigating ...
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng   +7 more
doaj   +1 more source

A PEROXO-Tag Enables Rapid Isolation of Peroxisomes from Human Cells

iScience, 2020
Summary: Peroxisomes are metabolic organelles that perform a diverse array of critical functions in human physiology. Traditional isolation methods for peroxisomes can take more than 1 h to complete and can be laborious to implement.
G. Jordan Ray, Elizabeth A. Boydston, Emily Shortt, Gregory A. Wyant, Sebastian Lourido, Walter W. Chen, David M. Sabatini   +6 more
doaj   +1 more source