Results 21 to 30 of about 568,188 (255)

A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia

iScience, 2021
Summary: Organoids mimic the physiologic and pathologic events of organs. However, no consensus on esophageal organoid (EO) culture methods has been reached.
Biyun Zheng, Kyung-Pil Ko, Xuefen Fang, Xiaozhong Wang, Jie Zhang, Sohee Jun, Bong-Jun Kim, Wenyi Luo, Moon Jong Kim, Youn-Sang Jung, Christopher L. Cervantes, Jae-Il Park   +11 more
doaj   +1 more source

PCR-based profiling of transcription factor activity in vivo by a virus-based reporter battery

iScience, 2022
Summary: Understanding the molecular mechanisms of gene regulation is pivotal for understanding how cells establish and modify their identities and functions.
Hitomi Abe, Kentaro Abe
doaj   +1 more source

Integrating extrusion complex-associated pattern to predict cell type-specific long-range chromatin loops

iScience, 2022
Summary: The chromatin loop plays a critical role in the study of gene expression and disease. Supervised learning-based algorithms to predict the chromatin loops require large priori information to satisfy the model construction, while the prediction ...
Yajing Deng, Li Tang, Xiaolong Zhou, Wenkang Wang, Min Li   +4 more
doaj   +1 more source

A human multi-cellular model shows how platelets drive production of diseased extracellular matrix and tissue invasion

iScience, 2021
Summary: Guided by a multi-level “deconstruction” of omental metastases, we developed a tetra (four cell)-culture model of primary human mesothelial cells, fibroblasts, adipocytes, and high-grade serous ovarian cancer (HGSOC) cell lines.
Beatrice Malacrida, Sam Nichols, Eleni Maniati, Roanne Jones, Robin Delanie-Smith, Reza Roozitalab, Eleanor J. Tyler, Morgan Thomas, Gina Boot, Jonas Mackerodt, Michelle Lockley, Martin M. Knight, Frances R. Balkwill, Oliver M.T. Pearce   +13 more
doaj   +1 more source

A quantitative micro-tomographic gut atlas of the lepidopteran model insect Manduca sexta

iScience, 2023
Summary: The tobacco hornworm is used extensively as a model system for ecotoxicology, immunology and gut physiology. Here, we established a micro-computed tomography approach based on the oral application of the clinical contrast agent iodixanol ...
Anton G. Windfelder, Jessica Steinbart, Ulrich Flögel, Jan Scherberich, Marian Kampschulte, Gabriele A. Krombach, Andreas Vilcinskas   +6 more
doaj   +1 more source

Spatio-temporal optical coherence tomography provides full thickness imaging of the chorioretinal complex

iScience, 2022
Summary: Despite the rapid development of optical imaging methods, high-resolution in vivo imaging with penetration into deeper tissue layers is still a major challenge. Optical coherence tomography (OCT) has been used successfully for non-invasive human
Egidijus Auksorius, Dawid Borycki, Piotr Wegrzyn, Bartosz L. Sikorski, Kamil Lizewski, Ieva Zickiene, Mounika Rapolu, Karolis Adomavicius, Slawomir Tomczewski, Maciej Wojtkowski   +9 more
doaj   +1 more source

GATA1 deletion in human pluripotent stem cells increases differentiation yield and maturity of neutrophils

iScience, 2023
Summary: Human pluripotent stem cell (hPSC)-derived tissues can be used to model diseases in cell types that are challenging to harvest and study at-scale, such as neutrophils.
Thomas C. Harper, Elaine M. Oberlick, Tomas J. Smith, Duncan E. Nunes, Mark-Anthony Bray, Seonmi Park, Corey D. Driscoll, Sarah F. Mowbray, Christophe Antczak   +8 more
doaj   +1 more source

Wake respirometry allows breath-by-breath assessment of ventilation and CO2 production in unrestrained animals

iScience, 2022
Summary: Quantifying stress and energetic responses in animals are major challenges, as existing methods lack temporal resolution and elevate animal stress.
Kayleigh A.R. Rose, Rory P. Wilson, Claudia Ramenda, Hermina Robotka, Martin Wikelski, Emily L.C. Shepard   +5 more
doaj   +1 more source

European Standard Clinical Practice Guideline and EXPeRT Recommendations for the Diagnosis and Management of Gastroenteropancreatic Neuroendocrine Neoplasms in Children and Adolescents

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Pediatric gastroenteropancreatic neuroendocrine neoplasms (GEP‐NENs) are extremely rare and clinically heterogeneous. Management has largely been extrapolated from adult practice. This European Standard Clinical Practice Guideline (ESCP), developed by the EXPeRT network in collaboration with adult NEN experts, provides (adult) evidence ...
Michaela Kuhlen, Calogero Virgone, Antje Redlich, Michael Abele, Ricardo Lopez‐Almaraz, Tal Ben‐Ami, Ewa Bien, Gianni Bisogno, Maja Mazic Cesen, Andrea Ferrari, Malgorzata A. Krawczyk, Yves Reguerre, Dominik T. Schneider, Ines B. Brecht, Charlotte Rigaud, Bilgehan Yalcin, Rainer Claus, Christian Vokuhl, Constantin Lapa, Jörg Fuchs, Segolene Hescot, Thomas Walter, Julien Hadoux, Daniel Orbach   +23 more
wiley   +1 more source

Seeing Natural Images through the Eye of a Fly with Remote Focusing Two-Photon Microscopy

iScience, 2020
Summary: Visual systems of many animals, including the fruit fly Drosophila, represent the surrounding space as 2D maps, formed by populations of neurons. Advanced genetic tools make the fly visual system especially well accessible.
Anna Schuetzenberger, Alexander Borst
doaj   +1 more source