Results 51 to 60 of about 491,032 (299)

HIV-1 env gene mutations outside the targeting probe affects IPDA efficiency

iScience
Summary: The intact proviral DNA assay (IPDA) based on droplet digital PCR was developed to identify intact proviral DNA and quantify HIV-1 latency reservoirs in patients infected with HIV-1.
Zehua Zhou, Xinyu Zhang, Meiyu Wang, Fei Jiang, Jincheng Tong, Jianhui Nie, Chenyan Zhao, Haifa Zheng, Zhen Zhang, Penghui Shi, Weiguang Fan, Youchun Wang, Weijin Huang   +12 more
doaj   +1 more source

Biological and technical complexities in analyzing extracellular vesicle immune interactions in B‐cell malignancies

FEBS Letters, EarlyView.
Extracellular vesicles (EVs) mediate intercellular communication in tumor immune microenvironments. However, their role in B‐cell malignancies remains poorly defined, owing to biological complexity and technical challenges in EV isolation and analysis.
Daniel Bachurski, Michael Hallek
wiley   +1 more source

METODOLOGIAS ATIVAS À LUZ DO ENFOQUE CTS: ALTERNATIVAS PARA O ENSINO-APRENDIZAGEM DE CIÊNCIAS BIOLÓGICAS A PARTIR DE UMA QUESTÃO SOCIOCIENTÍFICA

Professare, 2023
O uso de metodologias ativas (MA) e o ensino com enfoque Ciência, Tecnologia e Sociedade (CTS) podem ser meios para estimular o processo de ensino-aprendizagem ativo e crítico. O presente estudo objetivou avaliar a contribuição das estratégias ativas, como o Role play e a Rotação por Estações de Aprendizagem, para o ensino sob a perspectiva CTS na ...
Aline Lubyi LUBYI, Diovana Aparecida Carvalho da Silva, Camila Juraszeck Machado, Josi Mariano Borille   +3 more
openaire   +1 more source

Application of isothermal titration calorimetry in the biological sciences: things are heating up!

BioTechniques, 2004
During the last 15 years isothermal titration calorimetry (ITC) has come of age. There are now in excess of 2000 instruments sited in laboratories in more than 40 countries around the world.
J. Ladbury
semanticscholar   +1 more source

Goodbye flat lymphoma biology

FEBS Letters, EarlyView.
Three‐dimensional (3D) biological systems have become key tools in lymphoma research, offering reliable in vitro and ex vivo platforms to explore pathogenesis and support precision medicine. This review highlights current 3D non‐Hodgkin lymphoma models, detailing their features, advantages, and limitations, and provides a broad perspective on future ...
Carla Faria, Cèlia Dobaño‐López, Patricia Pérez‐Galán, Christine Bezombes   +3 more
wiley   +1 more source

Akaluc bioluminescence offers superior sensitivity to track in vivo dynamics of SARS-CoV-2 infection

iScience
Summary: Monitoring in vivo viral dynamics can improve our understanding of pathogenicity and tissue tropism. Because the gene size of RNA viruses is typically small, NanoLuc is the primary choice for accommodation within viral genome.
Tomokazu Tamura, Hayato Ito, Shiho Torii, Lei Wang, Rigel Suzuki, Shuhei Tsujino, Akifumi Kamiyama, Yoshitaka Oda, Masumi Tsuda, Yuhei Morioka, Saori Suzuki, Kotaro Shirakawa, Kei Sato, Kumiko Yoshimatsu, Yoshiharu Matsuura, Satoshi Iwano, Shinya Tanaka, Takasuke Fukuhara   +17 more
doaj   +1 more source

Ultra-high-throughput Ca2+ assay in platelets to distinguish ITAM-linked and G-protein-coupled receptor activation

iScience, 2022
Summary: Antiplatelet drugs targeting G-protein-coupled receptors (GPCRs), used for the secondary prevention of arterial thrombosis, coincide with an increased bleeding risk. Targeting ITAM-linked receptors, such as the collagen receptor glycoprotein VI (
Delia I. Fernández, Isabella Provenzale, Hilaire Y.F. Cheung, Jan van Groningen, Bibian M.E. Tullemans, Alicia Veninga, Joanne L. Dunster, Saman Honarnejad, Helma van den Hurk, Marijke J.E. Kuijpers, Johan W.M. Heemskerk   +10 more
doaj   +1 more source

METHODOLOGY OF USING MODERN APPROACHES AND INNOVATIONS IN TEACHING BIOLOGICAL SCIENCE IN UNIVERSAL EDUCATION SCHOOLS

, 2023
This article explores the methodology and implementation of contemporary approaches and innovations in teaching biological science within the context of universal education schools. With the rapid advancements in science and technology, educators are continually seeking effective strategies to engage students and enhance their learning experiences in ...
openaire   +2 more sources

Exploring High-Dimensional Biological Data with Sparse Contrastive Principal Component Analysis

bioRxiv, 2019
Motivation Statistical analyses of high-throughput sequencing data have re-shaped the biological sciences. In spite of myriad advances, recovering interpretable biological signal from data corrupted by technical noise remains a prevalent open problem ...
Philippe Boileau, N. Hejazi, S. Dudoit
semanticscholar   +1 more source

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

FEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan, Lu Qi, Pierre Zalloua   +2 more
wiley   +1 more source