Results 161 to 170 of about 1,319,865 (263)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Sleep quality in Olympic and non-Olympic combat sports: associations with sex, rapid weight loss, and sport age (a PSQI-based analysis). [PDF]

Front Psychol
Eroğlu Ş, Ceylan T, Türkmen M, Yılmaz T, Imamoğlu O, Kara I, Sekban G, Sahin FN.   +7 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Aptamer-based analyses of plasma proteome in individuals with post-COVID condition who underwent tailored physical activity. [PDF]

Front Sports Act Living
Chowdhury MMH, Fontaine MN, Lord SE, Lucier JF, Allard-Chamard H, Ilangumaran S, Piché A, Dionne IJ, Ramanathan S.   +8 more
europepmc   +1 more source

A Science Cloud as the Forth Methodology for Science

TRENDS IN THE SCIENCES, 2012
openaire   +2 more sources

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Objective Analysis of Movement Behaviours and Cardiometabolic Health in Sub-Saharan Africans Living in the UK: A Cross Sectional Study. [PDF]

Health Sci Rep
Ibirogba DA, Andalco LS, Crotti M, Charman SJ, Fuller AS, Ajepe T, Duncan M, Blain AP, Niyi-Odumosu F, Mwangi PW, Bukachi FO, Dominic OL, Barak OF, Jakovljevic DG, Okwose NC.   +14 more
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Clinical and economic impact of incisional negative pressure wound therapy in breast surgery: meta-analysis. [PDF]

Br J Surg
Kollatos C, Sackey H, Pantiora E, Vorburger D, Rocco N, Öhrn C, Valachis A, Eriksson S, Karakatsanis A.   +8 more
europepmc   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source