Results 91 to 100 of about 1,906,800 (219)

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Inter-laboratory optimization for the detection of <i>Campylobacter jejuni</i> in canine fecal samples using <i>gyrA</i> PCR. [PDF]

J Vet Diagn Invest
Lawhon SD, Cummings KJ, Wu J, Yang CY, Whipple N, Prarat Koscielny M, Lindemann S, Kiener S, Kmet M, Reddy R, Goodman LB, Franklin-Guild RJ, Sams K, Cronk B, Cui J, Lawrence K, Zhang Q, Zhang Y, Ensley S, Olsen K, Tomaszewski J, Shen Z, Zhang S, Dasgupta S, Mann S, Patil A, Thakur S, Rankin SC, Ruesch L, Garcia-Fernandez N, Guag J, Nemser SM, Reimschuessel R.   +32 more
europepmc   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Target product profiles of laboratory and data analytical frameworks for genotyping to monitor antimalarial efficacy. [PDF]

PLOS Glob Public Health
Plucinski MM, Wesolowski A, Gerlovina I, Taylor AR, Briggs J, Aranda-Díaz A, Golumbeanu M, Bajic M, Bailey JA, Barratt JLN, Buckee C, Deme AB, Felger I, Ghansah A, Hastings I, Kattenberg JH, Mayor A, Menard D, Moriarty LF, Neafsey D, Okell L, Oyier I, Raman J, Rosenthal PJ, Rosanas-Urgell A, Verity R, Volkman SK, Nsanzabana C, Greenhouse B.   +28 more
europepmc   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

A High Sensitive Detection of Droplet Digital PCR for the Diagnosis of Pulmonary Tuberculosis. [PDF]

Infect Drug Resist
Yang S, Huang M, Xiong Y, Tu C, Xu Y, Wan K, Chen X, Zhang F, Liao X, Chen Q, Zhang J, Liu J.   +11 more
europepmc   +1 more source

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

Laboratory Diagnostics of Aspergillosis: Present State and Future Directions. [PDF]

J Fungi (Basel)
Tomazin R, Matos T.
europepmc   +1 more source

Circular RNA expression landscapes in myelodysplastic neoplasms: Associations with mutational signatures and disease progression

Molecular Oncology, EarlyView.
In this explorative study, the abundance of circular RNA molecules in bone marrow stem cells was found to be elevated in patients with high‐risk myelodysplastic neoplasms, and to be associated with an increased risk of progression to acute myeloid leukemia.
Eileen Wedge, Morten Tulstrup, Gabriele Todisco, Pedro Moura, Christophe Côme, Jakob Werner Hansen, Jakob Schmidt Jespersen, Balthasar Schlotmann, Maria Creignou, Mette Dahl, Claudia Schöllkopf, Klas Raaschou‐Jensen, Krister Wennerberg, Bo Porse, Joachim Weischenfeldt, Eva Hellström‐Lindberg, Lasse Sommer Kristensen, Kirsten Grønbæk   +17 more
wiley   +1 more source