Results 71 to 80 of about 1,906,800 (219)

An Evidence Atlas of Diagnostic Tests for Genitourinary Tuberculosis: A Laboratory-Centered Framework. [PDF]

Int J Gen Med
Zeng H, Zhang Q.
europepmc   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Unexpected Variability in Laboratory and Clinical Assays Used to Quantify Cryptococcus neoformans Polysaccharide. [PDF]

J Infect Dis
Wear MP, Kim D, Roy R, Zhang S, Casadevall A.   +4 more
europepmc   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Evolving microbiology laboratories: mastering automated culture-based processes and molecular assays, an institutional experience. [PDF]

Front Cell Infect Microbiol
Cherkaoui A, Renzi G, Fischer A, Tittel-Elmer M, Tognon M, François P, Lazarevic V, Schrenzel J.   +7 more
europepmc   +1 more source

Molecular methods applied to gynecological diseases: a laboratory review

Revista da Associação Médica Brasileira
Kátia Candido Carvalho, Giovanna dos Santos Cavalcanti, Grazielle Marques Duarte Purcino, Tatielly Teles de Miranda, Ana Carolina da Costa Sousa, Giovanna Ney Quevedo, Laura Gonzalez dos Anjos, José Maria Soares Junior, Edmund Chada Baracat   +8 more
openaire   +3 more sources

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Correction: Li et al. The Anthraquinone Derivative C2 Enhances Oxaliplatin-Induced Cell Death and Triggers Autophagy via the PI3K/AKT/mTOR Pathway. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6468. [PDF]

Int J Mol Sci
Li Y, Yan W, Qin Y, Zhang L, Xiao S.
europepmc   +1 more source

Molecular methods in the diagnostic laboratory: when to start and where to stop [PDF]

Clinical Microbiology and Infection, 1999
openaire   +1 more source