Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A variable neighborhood search with an effective local search for uncapacitated multilevel lot-sizing problems [PDF]
In this study, we improved the variable neighborhood search (VNS) algorithm for solving uncapacitated multilevel lot-sizing (MLLS) problems. The improvement is two-fold.
Xu, Yuchun +4 more
core +1 more source
Chapter 11. Problems and Problem Solving in the Light of Context-based Chemistry
To achieve higher-order thinking and meaningful deep learning, problem solving is fundamental for students to master. If one wants to make students engaged in their own learning and their problem-solving process, interesting and relevant tasks are a ...
Broman, Karolina,, Karolina Broman
core +1 more source
Pathways and pitfalls: a qualitative study of student experiences in biomedical science education
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell +8 more
wiley +1 more source
Radial basis function based meshless methods for fluid flow problems
This thesis is concerned with the development of meshless methods using radial basis functions for solving fluid flow problems. The advantage of meshless methods over traditional mesh-based methods is that they make use of a scattered set of collocation ...
Chinchapatnam, Phani P.
core +1 more source
TRAVEL OF A CIRCLE IN A TRIANGLE, AND THE TRIANGLE IN THE HOLLOW OR SELF COMPUTER DIRECTOR
The article describes an unusual possibility to use animation to study numerical methods for solving mathematical problems. Animation is defi ned not only and not so much as the creation of animation clips, but direct «revival» of the results of the ...
Valerij F. Ochkov, Elena P. Bogomolova
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Modern Methods for Solving Nonconvex Optimal Control Problems
The paper presents a few remarks on the evolution of Irkutsk’s school of O. V. Vasiliev on optimal control methods based on Pontryagin principle. Besides, one reviews some features of Pontryagin principle, in particular, its sufficiency and constructive ...
A.S. Strekalovsky
doaj
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
Two New Extended PR Conjugate Gradient Methods for Solving Nonlinear Minimization Problems [PDF]
In this paper, we have discussed and investigated two nonlinear extended PR-CG method which use function and gradient values. The two new methods involve the standard CG-methods and have the sufficient descent and globally convergence properties under ...
Abbas AL-Bayati, Rana AL-Kawaz
doaj +1 more source

