Results 1 to 10 of about 1,761,887 (298)
A communal catalogue reveals Earth’s multiscale microbial diversity
Nature, 2017 The spontaneous deamination of cytosine is a major source of transitions from C•G to T•A base pairs, which account for half of known pathogenic point mutations in humans.
Nicole M. Gaudelli +6 more
semanticscholar +1 more source
Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences
Nature Biotechnology, 2013 Profiling phylogenetic marker genes, such as the 16S rRNA gene, is a key tool for studies of microbial communities but does not provide direct evidence of a community's functional capabilities.
M. Langille +11 more
semanticscholar +1 more source
PLoS ONE, 2014 Advances in modern sequencing technologies allow us to generate sufficient data to analyze hundreds of bacterial genomes from a single machine in a single day.
B. J. Walker +10 more
semanticscholar +1 more source
A human gut microbial gene catalogue established by metagenomic sequencing
Nature, 2010 To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial ...
J. Qin +52 more
semanticscholar +1 more source
bioRxiv, 2020 Culture-independent analyses of microbial communities have advanced dramatically in the last decade, particularly due to advances in methods for biological profiling via shotgun metagenomics.
F. Beghini +15 more
semanticscholar +1 more source
Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
The ISME Journal, 2012 DNA sequencing continues to decrease in cost with the Illumina HiSeq2000 generating up to 600 Gb of paired-end 100 base reads in a ten-day run. Here we present a protocol for community amplicon sequencing on the HiSeq2000 and MiSeq Illumina platforms ...
J. Caporaso +15 more
semanticscholar +1 more source
Phylogenetic identification and in situ detection of individual microbial cells without cultivation.
Microbiological reviews, 1995 The frequent discrepancy between direct microscopic counts and numbers of culturable bacteria from environmental samples is just one of several indications that we currently know only a minor part of the diversity of microorganisms in nature.
R. Amann, W. Ludwig, K. Schleifer
semanticscholar +1 more source
Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
Nature, 2019 Inflammatory bowel diseases, which include Crohn’s disease and ulcerative colitis, affect several million individuals worldwide. Crohn’s disease and ulcerative colitis are complex diseases that are heterogeneous at the clinical, immunological, molecular,
J. Lloyd-Price +57 more
semanticscholar +1 more source
The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST)
Nucleic Acids Res., 2013 In 2004, the SEED (http://pubseed.theseed.org/) was created to provide consistent and accurate genome annotations across thousands of genomes and as a platform for discovering and developing de novo annotations.
R. Overbeek +13 more
semanticscholar +1 more source
Microbiome, 2021 Advances in microbiome science are being driven in large part due to our ability to study and infer microbial ecology from genomes reconstructed from mixed microbial communities using metagenomics and single-cell genomics.
Zhichao Zhou +7 more
semanticscholar +1 more source