Results 1 to 10 of about 214,177 (262)

The Signal and the Noise: Characteristics of Antisense RNA in Complex Microbial Communities

mSystems, 2020
High-throughput sequencing has allowed unprecedented insight into the composition and function of complex microbial communities. With metatranscriptomics, it is possible to interrogate the transcriptomes of multiple organisms simultaneously to get an ...
Thomas Yssing Michaelsen, Jakob Brandt, Caitlin Margaret Singleton, Rasmus Hansen Kirkegaard, Johanna Wiesinger, Nicola Segata, Mads Albertsen   +6 more
doaj   +3 more sources

Connecting structure to function with the recovery of over 1000 high-quality metagenome-assembled genomes from activated sludge using long-read sequencing

Nature Communications, 2021
Microbes play key roles in wastewater treatment. Here, Singleton et al. use long-read and short-read sequencing to recover 1083 high-quality metagenome-assembled genomes from 23 wastewater treatment plants, and combine this information with amplicon data,
Caitlin M. Singleton, Francesca Petriglieri, Jannie M. Kristensen, Rasmus H. Kirkegaard, Thomas Y. Michaelsen, Martin H. Andersen, Zivile Kondrotaite, Søren M. Karst, Morten S. Dueholm, Per H. Nielsen, Mads Albertsen   +10 more
doaj   +1 more source

MiDAS 4: A global catalogue of full-length 16S rRNA gene sequences and taxonomy for studies of bacterial communities in wastewater treatment plants

Nature Communications, 2022
Microbial communities are responsible for biological wastewater treatment. Here, Dueholm et al. generate more than 5 million high-quality, full-length 16S rRNA gene sequences from wastewater treatment plants across the world to construct a database with ...
Morten Kam Dahl Dueholm, Marta Nierychlo, Kasper Skytte Andersen, Vibeke Rudkjøbing, Simon Knutsson, MiDAS Global Consortium, Mads Albertsen, Per Halkjær Nielsen   +7 more
doaj   +1 more source

A comprehensive overview of the Chloroflexota community in wastewater treatment plants worldwide

mSystems, 2023
Filamentous Chloroflexota are abundant in activated sludge wastewater treatment plants (WWTPs) worldwide and are occasionally associated with poor solid-liquid separation or foaming, but most of the abundant lineages remain undescribed.
Francesca Petriglieri, Zivile Kondrotaite, Caitlin Singleton, Marta Nierychlo, Morten K. D. Dueholm, Per H. Nielsen   +5 more
doaj   +1 more source

Influence of biofilm growth age, media, antibiotic concentration and exposure time on Staphylococcus aureus and Pseudomonas aeruginosa biofilm removal in vitro

BMC Microbiology, 2020
Background Biofilm is known to be tolerant towards antibiotics and difficult to eradicate. Numerous studies have reported minimum biofilm eradication concentration (MBEC) values of antibiotics for many known biofilm pathogens.
Xiaofeng Chen, Trine Rolighed Thomsen, Heinz Winkler, Yijuan Xu   +3 more
doaj   +1 more source

Reevaluation of the Phylogenetic Diversity and Global Distribution of the Genus “Candidatus Accumulibacter”

mSystems, 2022
“Candidatus Accumulibacter” was the first microorganism identified as a polyphosphate-accumulating organism (PAO) important for phosphorus removal from wastewater.
Francesca Petriglieri, Caitlin M. Singleton, Zivile Kondrotaite, Morten K. D. Dueholm, Elizabeth A. McDaniel, Katherine D. McMahon, Per H. Nielsen   +6 more
doaj   +1 more source

Generation of Comprehensive Ecosystem-Specific Reference Databases with Species-Level Resolution by High-Throughput Full-Length 16S rRNA Gene Sequencing and Automated Taxonomy Assignment (AutoTax)

mBio, 2020
High-throughput 16S rRNA gene amplicon sequencing is an essential method for studying the diversity and dynamics of microbial communities. However, this method is presently hampered by the lack of high-identity reference sequences for many environmental ...
Morten Simonsen Dueholm, Kasper Skytte Andersen, Simon Jon McIlroy, Jannie Munk Kristensen, Erika Yashiro, Søren Michael Karst, Mads Albertsen, Per Halkjær Nielsen   +7 more
doaj   +1 more source

Long-Read Metagenome-Assembled Genomes Improve Identification of Novel Complete Biosynthetic Gene Clusters in a Complex Microbial Activated Sludge Ecosystem

mSystems, 2022
Microorganisms produce a wide variety of secondary/specialized metabolites (SMs), the majority of which are yet to be discovered. These natural products play multiple roles in microbiomes and are important for microbial competition, communication, and ...
Roberto Sánchez-Navarro, Matin Nuhamunada, Omkar S. Mohite, Kenneth Wasmund, Mads Albertsen, Lone Gram, Per H. Nielsen, Tilmann Weber, Caitlin M. Singleton   +8 more
doaj   +1 more source

A Distinct, Flocculent, Acidogenic Microbial Community Accompanies Methanogenic Granules in Anaerobic Digesters

Microbiology Spectrum, 2021
The formation of dense, well-settling methanogenic granules is essential for the operation of high-rate, up-flow anaerobic bioreactors used for wastewater treatment.
Simon Mills, Anna Christine Trego, Piet N. L. Lens, Umer Zeeshan Ijaz, Gavin Collins   +4 more
doaj   +1 more source

Candidatus Amarolinea and Candidatus Microthrix Are Mainly Responsible for Filamentous Bulking in Danish Municipal Wastewater Treatment Plants

Frontiers in Microbiology, 2020
Filamentous bulking is a common serious operational problem leading to deteriorated sludge settling that has long been observed in activated sludge biological wastewater treatment systems.
Marta Nierychlo, Simon J. McIlroy, Simon J. McIlroy, Sergey Kucheryavskiy, Chenjing Jiang, Anja S. Ziegler, Zivile Kondrotaite, Mikkel Stokholm-Bjerregaard, Mikkel Stokholm-Bjerregaard, Per Halkjær Nielsen   +9 more
doaj   +1 more source