Results 61 to 70 of about 862,177 (279)

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source

Lab‐in‐a‐Tube System Integrating Hierarchically Wrinkled Electrodes and DNAzyme‐Functionalized Beads for Diagnosis of Clostridioides difficile Infection

Advanced Materials, EarlyView.
The point‐of‐care diagnosis of Clostridioides difficile infection remains challenging due to reliance on slow culture and amplification‐based methods. A lab‐in‐a‐tube electrochemical biosensor integrating DNAzymes, antifouling magnetic beads, and a hierarchically structured flow cell enables amplification‐ and culture‐free detection directly in stool ...
Survanshu Saxena, Soyeon Lee, Youngeun Choi, Jonathan L'Heureux‐Hache, Jiaying Liu, Sadman Sakib, Sara M. Imani, Marek Smieja, Yingfu Li, Todd Hoare, Leyla Soleymani   +10 more
wiley   +1 more source

Evaluation of MALDI-TOF for identification of Vibrio cholerae and Vibrio parahaemolyticus from growth on agar media

Applied Microbiology and Biotechnology
Two methods were compared for their ability to accurately identify Vibrio species of interest: whole genome sequencing as the reference method and MALDI-TOF MS (matrix-assisted laser desorption/ionization-time of flight mass spectrometry) proteome ...
Swapan Banerjee, Annika Flint, Madeleine B. Brosseau, Kelly Weedmark, Bojan Shutinoski   +4 more
doaj   +1 more source

Candida auris metabolism and growth preferences in physiologically relevant skin-like conditions

mBio
Candida auris is an opportunistic, multidrug-resistant yeast with a high capacity for human skin colonization in healthcare settings, which can lead to subsequent infections with high mortality rates.
Jonathan P. Nicklas, Clay Deming, ShihQueen Lee-Lin, Sean Conlan, Zeyang Shen, Ryan A. Blaustein, Julia A. Segre   +6 more
doaj   +1 more source

Fast and accurate average genome size and 16S rRNA gene average copy number computation in metagenomic data

BMC Bioinformatics, 2019
Background Metagenomics caused a quantum leap in microbial ecology. However, the inherent size and complexity of metagenomic data limit its interpretation.
Emiliano Pereira-Flores, Frank Oliver Glöckner, Antonio Fernandez-Guerra   +2 more
doaj   +1 more source

Transcription Factor Promiscuity Drives Regulatory Rewiring and Evolvability in Gene Networks in Bacteria

Advanced Science, EarlyView.
When a master transcription factor (TF) is lost, bacteria can rapidly rewire gene regulatory networks by co‐opting related regulators. Using experimental evolution in Pseudomonas fluorescens, we show that TF promiscuity (low‐level, non‐cognate binding) provides the raw material for rewiring. Successful co‐option follows a predictable hierarchy governed
Tiffany B. Taylor, Alan M. Rice
wiley   +1 more source

BME402 – Microbial Genomics (Genomik Mikrob) [PDF]

Second Semester Examination 2023/2024 Academic Session July/August 2024 BME402 – Microbial Genomics (Genomik Mikrob) Duration : 2 hours (Masa : 2 ...
PPSKJ, Pusat Pengajian Sains Kajihayat
core   +3 more sources

The hidden genetic reservoir: structural variants as drivers of marine microbial and viral microdiversity

Environmental Microbiome
Background Intraspecific genetic diversity is fundamental to understanding microbial adaptation, evolution, and contributions to ecosystem stability.
Jose M. Haro-Moreno, Juan J. Roda-Garcia, Carmen Molina-Pardines, Mario López-Pérez   +3 more
doaj   +1 more source

Inhibition of NHEJ repair by type II-A CRISPR-Cas systems in bacteria

Nature Communications, 2017
The double-strand breaks generated by CRISPR-Cas systems are the target of multiple DNA repair pathways. Here the authors find incompatibility between NHEJ and type II-A CRISPR-Cas systems due to Csn2 mediated inhibition of end-joining.
Aude Bernheim, Alicia Calvo-Villamañán, Clovis Basier, Lun Cui, Eduardo P. C. Rocha, Marie Touchon, David Bikard   +6 more
doaj   +1 more source

Single‐Cell Dissection of Therapy‐Induced Remodeling Uncovers a Fibroblast‐Driven Immunosuppressive Niche and Targetable Vulnerabilities in Lethal Prostate Cancer

Advanced Science, EarlyView.
Single‐cell longitudinal profiling reveals that androgen‐deprivation therapy induces a DPT+ fibroblast‐complement axis that suppresses macrophage inflammation and drives CD8+ T cell exhaustion in prostate cancer. Concurrently, resistant epithelial subpopulations persist and engage TSPAN1‐ and NRXN1‐mediated programs promoting CRPC and neuroendocrine ...
Yang Chen, Dandan Dong, Jinling Liao, Naikai Liao, Mengqi Liu, Qin Zhang, Siting Chen, Qi Liu, Ying Lu, Tianyu Li, Chunlin Zou, Qiong Song, Qiuyan Wang, Jian Liang, Qiji Xie, Chengyang Li, Mengyun Wang, Jiwen Cheng, Zengnan Mo, Gong‐Hong Wei   +19 more
wiley   +1 more source