Results 31 to 40 of about 1,811,890 (270)
Electroporation in Microfluidic Devices [PDF]
, 2016 Crossing the plasma cellular membrane for loading of exogenous substances or accessing the intracellular medium is essential for cell engineering and transfection, cell analysis, or controlled extraction of the cellular content. Various chemical and physical techniques have been developed to open up the cell membrane and allow molecular exchange ...
Le Gac, Séverine, van Uitert, Iris
openaire +3 more sources
Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
Functional microfluidics: theory, microfabrication, and applications
International Journal of Extreme ManufacturingMicrofluidic devices are composed of microchannels with a diameter ranging from ten to a few hundred micrometers. Thus, quite a small (10 ^−9 –10 ^−18 l) amount of liquid can be manipulated by such a precise system. In the past three decades, significant
Mingzhu Xie +6 more
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Advances in Nucleic Acid Amplification-Based Microfluidic Devices for Clinical Microbial Detection
Chemosensors, 2022 Accurate and timely detection of infectious pathogens is urgently needed for disease treatment and control of possible outbreaks worldwide. Conventional methods for pathogen detection are usually time-consuming and labor-intensive.
Thi Ngoc Diep Trinh, Nae Yoon Lee
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Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research
FEBS Letters, EarlyView.What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
wiley +1 more source
Categorising hybrid material microfluidic devices
Frontiers in Lab on a Chip TechnologiesMicrofluidic devices are useful tools for a wide range of biomedical, industrial, and environmental applications. Hybrid microfluidic devices utilising more than two materials are increasingly being used for their capacity to produce unique structures ...
Tom Carvell +3 more
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Fabrication of Transparent and Flexible Digital Microfluidics Devices
Micromachines, 2022 This study proposed a fabrication method for thin, film-based, transparent, and flexible digital microfluidic devices. A series of characterizations were also conducted with the fabricated digital microfluidic devices.
Jianchen Cai +6 more
doaj +1 more source
Molecular Oncology, EarlyView.Pair‐wise comparison of the CellSearch and FETCH enrichment technologies for circulating tumor cells (CTCs) from metastatic breast, prostate, and small cell lung cancer patients shows an increased capture of CTCs using FETCH enrichment. The clinical implementation of circulating tumor cells (CTCs) as a predictive tool for therapy efficacy in the ...
Michiel Stevens +6 more
wiley +1 more source
Open-source interactive design platform for 3D-printed microfluidic devices
Communications EngineeringMicrofluidics and 3D printing offer exciting opportunities for the development of new technologies and applications in the fields of biology, chemistry, and medicine.
Yushen Zhang +3 more
doaj +1 more source
Double-Sided Tape in Microfluidics: A Cost-Effective Method in Device Fabrication
BiosensorsThe demand for easy-to-use, affordable, accessible, and reliable technology is increasing in biological, chemical, and medical research. Microfluidic devices have the potential to meet these standards by offering cost-effective, highly sensitive, and ...
Savanah Smith +2 more
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