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Microinjection of calcium into droplets of aequorin
Proceedings of the Royal Society of London. Series B. Biological Sciences, 1975Calcium ions were microinjected, ionophoretically or by pressure, into droplets of the photoprotein aequorin. For pulses less than about 1 s in duration, the amplitude and rate of rise of the light emitted as a result of the combination of calcium with aequorin, were directly proportional to the ionophoretic current flowing through the Ca pipette ...
Ricardo Miledi, K. Kusano, J. Stinnakre
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Microinjection into crane-fly spermatocytes
Biochemistry and Cell Biology, 1993We were successful in microinjecting fluorescently labelled material into crane-fly spermatocytes. In our experiments, we obtained four results. (i) In most attempts, the membrane stretched around the micropipette and prevented entry of fluorescent material, even when the micropipette appeared to be pushed completely through the cell.
Arthur Forer +2 more
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Rapid Microinjection of Fertilized Eggs
2004The chapter focuses on methods for using a picospritzer to inject large numbers of eggs that have been immobilized in open plastic dishes. The microinjection of reagents into eggs and early embryos aids in analyzing many cellular and developmental processes and is essential in studying cell lineages and cell fate specification, gene regulation, gene ...
Charles A. Ettensohn, Melani S. Cheers
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Development of a Vibratory Microinjection Method
The International Journal of Artificial Organs, 2003To reduce cellular damage by pronuclear microinjection and nuclear transfer, we have recently developed a vibratory microinjection method. A micropipette was fixed to a piezoelectric ceramic with a resonance frequency of 70 kHz. When this micropipette was vibrated, it easily entered a mouse-fertilized egg without any sharp depression of the cell body,
Y Arai +5 more
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Microinjection - a tool to study gravitropism
Advances in Space Research, 2003Despite extensive studies on plant gravitropism this phenomenon is still poorly understood. The separation of gravity sensing, signal transduction and response is a common concept but especially the mechanism of gravisensing remains unclear. This paper focuses on microinjection as powerful tool to investigate gravisensing in plants.
Karl H. Hasenstein, P. Scherp
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Implantation of Microinjected Eggs
2003Microinjected eggs are normally incubated overnight in Ml6 microdrop culture at 37°C in CO₂ until 1400 h. By this time the eggs will have developed as far as the two-cell stage. The eggs are then implanted into surrogate mothers. As the membrane covering the oviduct of the rat is much tougher than the mouse, we normally cut it with a very fine pair of ...
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Microinjection of RNA into Mouse Zygotes
Cold Spring Harbor Protocols, 2018This protocol describes the injection of RNA into zygotes.
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Chapter 25 Microinjection of Tetrahymena thermophila
1999Publisher Summary Microinjection has proved to be a powerful experimental tool used to introduce macromolecules into cells. The method is widely used for DNA-mediated transformation of diverse organisms including ciliates, nematodes, insects, and mammals.
Douglas L. Chalker +3 more
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Microinjection and transgenesis
Biochimie, 1998Alejandro García-Carrancá +1 more
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Isolation of DNA Fragments for Microinjection
2003The purification of a DNA fragment for microinjection is extremely important. This chapter describes a rapid and efficient technique for isolating specific DNA fragments from agarose gels run in Tris-acetate buffer, and was first described by Vogelstein and Gillespie.
Smith, D.R., Murphy, D.
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