Results 11 to 20 of about 882,142 (268)

Electron Microscopy [PDF]

open access: yes, 2012
Correlative light (LM) and transmission electron microscopic (TEM) analysis is useful, if ultrastructural details of cells need to be related to functional aspects which can only be examined at the LM level. The first protocol presented here introduces a relatively simple way of obtaining TEM images which, on the one hand, reveal ultrastructural ...
Luder, H U, Amstad-Jossi, M
openaire   +3 more sources

Volume electron microscopy

open access: yesNature Reviews Methods Primers, 2022
Life exists in three dimensions, but until the turn of the century most electron microscopy methods provided only 2D image data. Recently, electron microscopy techniques capable of delving deep into the structure of cells and tissues have emerged, collectively called volume electron microscopy (vEM).
Christopher J. Peddie   +13 more
openaire   +4 more sources

Electron Microscopy of the Sarcolemma [PDF]

open access: yesNature, 1948
STRIATED muscle fibres are surrounded by a delicate membrane or sheath, the sarcolemma. It has frequently been stated1,2,3 that this sheath is composed of a network of collagenous or reticular fibrils, arranged like a knitted sock. One of us (R. B.) in an investigation to be described elsewhere has failed to find any evidence of fibrillar structure ...
W M, JONES, R, BARER
openaire   +2 more sources

SCANNING ELECTRON MICROSCOPY [PDF]

open access: yesJournal of the Royal Microscopical Society, 1964
Abstract Scanning electron microscopy is a method of microscopy that permits resolution better than that of the optical microscope (about 100 A) while examining one surface of a bulk specimen. The technique depends on electronic application to microscopy and has been developed over many years by electronic engineers rather than physicists or ...
openaire   +2 more sources

Multi-step atomic mechanism of platinum nanocrystals nucleation and growth revealed by in-situ liquid cell STEM

open access: yesScientific Reports, 2021
The understanding of crystal growth mechanisms has broadened substantially. One significant advancement is based in the conception that the interaction between particles plays an important role in the growth of nanomaterials.
Walid Dachraoui   +3 more
doaj   +1 more source

Endocytosis-like DNA uptake by cell wall-deficient bacteria

open access: yesNature Communications, 2022
Horizontal gene transfer in bacteria can occur through mechanisms such as conjugation, transduction and transformation, which facilitate the passage of DNA across the cell wall. Here, Kapteijn et al. show that cell wall-deficient bacteria can take up DNA
Renée Kapteijn   +10 more
doaj   +1 more source

Simulation of bonding effects in HRTEM images of light element materials

open access: yesBeilstein Journal of Nanotechnology, 2011
The accuracy of multislice high-resolution transmission electron microscopy (HRTEM) simulation can be improved by calculating the scattering potential using density functional theory (DFT) .
Simon Kurasch   +4 more
doaj   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

open access: yesFEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Biophysical approaches for studying viral entry

open access: yesFEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

Assembly mechanism of the pleomorphic immature poxvirus scaffold

open access: yesNature Communications, 2022
Immature poxviruses are characterized by nonicosahedral semiordered protein scaffolds critical for morphogenesis. Here, the authors use cryo-EM structures of Vaccinia virus D13 scaffold intermediates to explain their assembly mechanism.
Jaekyung Hyun   +3 more
doaj   +1 more source

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