Results 231 to 240 of about 160,406 (282)

Optimizing 3D multiphoton fluorescence microscopy

Optics Letters, 2013
We present a new optimization concept for 3D multiphoton fluorescence microscopy by finding the optimal excitation beam giving rise to the smallest possible light-emitting volume or the highest possible signal to noise ratio (SNR).
Ido Kaminer, Jonathan Nemirovsky, Mordechai Segev   +2 more
openaire   +2 more sources

Multiphoton fluorescence microscopy in biology

SPIE Proceedings, 2002
The inherent advantages of nonlinear excitation make multiphoton fluorescence microscopy (MPFM) awell-suited imaging technique for extracting valuable information from turbid and thick biological samples. These advantages include high three-dimensional spatial resolution, large penetration depth, minimum out-of-focus cellular photodamage, and high ...
Ahmed A. Heikal, Watt W. Webb
openaire   +1 more source

Multiphoton microscopy and fluorescence lifetime imaging of the rat and patient liver with cirrhosis ex vivo

BiOS, 2019
Cirrhosis is defined as the histological development of regenerative nodules surrounded by fibrous bands in response to chronic liver injury that leads to portal hypertension and end stage liver disease.
D. Kuznetsova, S. Rodimova, V. Dudenkova, N. Bobrov, N. М. Kiselev, V. Zagainov, E. Zagaynova   +6 more
semanticscholar   +1 more source

Multimodal optoacoustic and multiphoton fluorescence microscopy

SPIE Proceedings, 2013
Multiphoton microscopy is a powerful imaging modality that enables structural and functional imaging with cellular and sub-cellular resolution, deep within biological tissues. Yet, its main contrast mechanism relies on extrinsically administered fluorescent indicators.
Gali Sela, Daniel Razansky, Shy Shoham
openaire   +1 more source

A near-infrared I emissive dye: toward the application of saturable absorber and multiphoton fluorescence microscopy in the deep-tissue imaging window.

Chemical Communications, 2019
A boron-dipyrromethene (BODIPY) dye emitting in the near-infrared (NIR) I region (723 nm) exhibits strong saturable absorption at 680 nm. Its multiphoton absorption spectra in the NIR II and III regions are determined.
Can Ren, Xiangquan Deng, Wenbo Hu, Junzi Li, Xiaofei Miao, Shuyu Xiao, Hongji Liu, Quli Fan, Ke Wang, Tingchao He   +9 more
semanticscholar   +1 more source

Three-dimensional deep tissue multiphoton frequency-domain fluorescence lifetime imaging microscopy via phase multiplexing and adaptive optics

BiOS, 2019
We propose and demonstrate a novel multiphoton frequency-domain fluorescence lifetime imaging microscopy (MPM-FD-FLIM) system that is able to generate 3D lifetime images in deep scattering tissues.
Yide Zhang, Ian H. Guldner, Evan L. Nichols, David Benirschke, Cody J. Smith, Siyuan Zhang, S. Howard   +6 more
semanticscholar   +1 more source

Principles of Multiphoton-Excitation Fluorescence Microscopy

Cold Spring Harbor Protocols, 2007
INTRODUCTIONFluorescence microscopy has been gaining importance in quantitative biological research due to dramatic improvements in fluorophores, optical systems, light sources, and detectors. In particular, confocal fluorescence microscopy, usually by laser scanning, has for the first time allowed the observation of biological processes with high ...
openaire   +3 more sources

Optimizing Fluorescence Collection in Multiphoton Microscopy

Biomedical Optics, 2008
Efficient fluorescence collection is critically important when maximizing imaging depth in multiphoton microscopy. Here we present an optimized, large-aperture fluorescence collection system for use with Hamamatsu GaAsP photomultiplier tubes.
Joseph P. Zinter, Michael J. Levene
openaire   +1 more source

Multiphoton Fluorescence Microscopy

2014
Chapter 8 is dedicated to multiphoton excitation microscopy and specifically we focused on two-photon fluorescence microscopy. We employ quantum mechanical formalism along with perturbation theory to calculate the transition probabilities for single-photon, two-photon and in general n-photon excitation process. Transition probabilities explaining multi-
Partha Pratim Mondal, Alberto Diaspro
openaire   +1 more source

Fine Tuning of Multiphoton AIE Emission Behavior, Organelle Targeting, and Fluorescence Lifetime Imaging of Terpyridine Derivatives by Alkyl Chain Engineering.

Analytical Chemistry, 2022
In this work, a series of multiphoton terpyridine agents (ZA, ZA-Mex, and ZA-Hex) for fluorescence lifetime imaging microscopy (FLIM) are designed and synthesized.
Shujing Wang, Ying Lin, Chengkai Zhang, Tong Zhu, Xiaohe Tian, Dandan Li, Wen Ma, Qiong Zhang, Jieying Wu, Yu-peng Tian   +9 more
semanticscholar   +1 more source