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Characterization of In Vitro Ovarian Tissue Fluorescence with Multiphoton Microscopy
Frontiers in Optics, 2005The distribution of endogenous fluorescence in the ovary provides diagnostically useful information and aids in discriminating between normal and diseased tissue. Optically sliced, viable tissue provided correlative data for a clinical spectroscopic instrument.
Nathaniel D. Kirkpatrick +2 more
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Physical and biological limitations of in vivo multiphoton fluorescence microscopy
SPIE Proceedings, 2005In this paper we present experimental and theoretical study of limitations on deep imaging of biological tissue structure using multiphoton fluorescence microscopy (MFM), that are imposed by scattering of short NIR pulses in optically turbid medium. The time stretching of the laser pulse and its transversal widening limit the maximum imaging depth of ...
Alexey I. Korytin +3 more
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Investigation of depilatory mechanism by use of multiphoton fluorescent microscopy
Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 2007Transdermal drug delivery provides a non-invasive route of drug administration, and can be a alternative method to oral delivery and injection. The stratum corneum (SC) of skin acts as the main barrier to transdermal drug delivery.
Chiao-Ying Lin +4 more
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Image Formation in Multiphoton Fluorescence Microscopy∗
1998Abstract This chapter is dedicated to image formation in multiphoton fluorescence microscopy. In particular, the comparison of image formation is based on the three-dimensional intensity point spread function (IPSF), the three-dimensional optical transfer function (OTF), and axial and transverse image resolution for thin and thick ...
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Miniature three-photon microscopy maximized for scattered fluorescence collection
Nature Methods, 2023Chunzhuo Zhao +14 more
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Improved laser scanning fluorescence microscopy by multiphoton excitation
2000Publisher Summary The compound optical microscope has been central to countless advances in biological research. The growth of cell and molecular biology in recent years has forced the pace of microscopy research to provide high contrast images of increasingly more specific structures and functions in biological specimens.
N.S. White, R.J. Errington
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In-vivo Multiphoton Excited Fluorescence Microscopy of the Spinal Cord
2018Spinal cord disorders are frequent causes of morbidity and mortality in human and veterinary medicine alike. Despite spinal cord disease affecting nearly every vertebrate species on earth, the pathophysiological mechanisms of central nervous system (CNS) disease remain poorly understood, largely due to failure of traditional ex-vivo imaging methods to ...
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3D printing and bioprinting using multiphoton lithography
Bioprinting, 2020Alexander K Nguyen, Roger J Narayan
exaly

