Results 241 to 250 of about 160,406 (282)

Perovskites as Multiphoton Fluorescence Contrast Agents for In Vivo Imaging.

ACS Applied Materials and Interfaces
Multiphoton fluorescence microscopy is a powerful tool for imaging and exploring biological tissue at subcellular spatial resolution while minimizing photobleaching and autofluorescence.
Peuli Nath, A. Liversage, Luke J. Mortensen, Aniruddha Ray   +3 more
semanticscholar   +1 more source

Multifocal multiphoton fluorescence lifetime microscopy for biomedical applications

SPIE Proceedings, 2005
Two-photon microscopy is a key method for biological and medical research on cells and tissues mainly due to the submicronic spatial resolution. Unfortunately in its conventional form, this technique leads to long time recording for three-dimensional and fluorescence lifetime imaging because it requires a single point laser scanning.
Ariane Deniset, Sandrine Lévêque-Fort, Marie-Pierre Fontaine-Aupart, Gérard Roger, Patrick Georges   +4 more
openaire   +1 more source

Highly efficient upconverters for multiphoton fluorescence microscopy

Journal of Microscopy, 1998
A new generation of efficient two‐photon absorbing fluorescent molecules has been developed and used effectively for two‐photon laser scanning microscopy. Several examples of the use of these new fluorophores have been presented. In addition, issues relating to the two‐photon absorption cross‐section, excitation power, sample properties and resolution ...
null Cheng, null Pan, null Shih, null Kim, null Liou, null Park   +5 more
openaire   +1 more source

Multiphoton fluorescence microscopy for functional imaging of biomolecules

Nonlinear Optics: Materials, Fundamentals and Applications, 2002
Molecular level understanding of numerous biological processes can be elucidated using multiphoton fluorescence spectroscopy, dynamics, and microscopy. We exploit the intrinsic biomolecular fluorescence in live cells for non-invasive and real-time monitoring of in vivo biological processes including the mitochondrial respiratory chain.
Ahmed A. Heikal, Watt W. Webb
openaire   +1 more source

In vivo multiphoton fluorescence microscopy of epithelial precancer

SPIE Proceedings, 2011
Most human cancers arise from epithelium, the superficial layer covering the exterior of body or lining the internal body cavities. Endogenous fluorophores such as aromatic amino acids, reduced nicotinamide adenine dinucleotide (NADH), flavoprotein (FAD), keratin, collagen, and elastin can provide abundant information to reveal the changes in ...
Wei Zheng, Dong Li, Yan Zeng, Jianan Y. Qu   +3 more
openaire   +1 more source

Nanosecond laser multiphoton scanning fluorescence microscopy

2006 Conference on Lasers and Electro-Optics and 2006 Quantum Electronics and Laser Science Conference, 2006
A high-rep-rate Q-switched Nd-YAG laser is used to obtain non-destructive multiphoton-excited laser scanning fluorescence images of a lithographic 2D pattern of polymeric samples on glass with a resolution of
null Tse-Luen Wee, null Shu Wei Huang, A. H. Kung   +2 more
openaire   +1 more source

Multispot point spread function for multiphoton fluorescence microscopy

Review of Scientific Instruments, 2009
We propose and demonstrate an imaging technique capable of generating multiple excitation spot for multiphoton fluorescence microscopy. The point spread function (PSF) is generated by interfering two counterpropagating extended depth of focus beams along the optical axis.
openaire   +2 more sources

High-Speed Simultaneous in vivo Multiphoton Microscopy and Fluorescence Lifetime Microscopy

Biomedical Optics and 3-D Imaging, 2012
We present full-frame (256x500 pixel) simultaneous multiphoton and fluorescence lifetime microscopy, in vivo. Parallel excitation and collection on a single element detector allows for a two orders of magnitude increase in pixel rate over serial scanning and deep imaging in scattering medium.
Scott S. Howard, Adam Straub, Chris Xu
openaire   +1 more source

Multiphoton fluorescence microscopy for biomedical imagining

IEE Colloquium on Biomedical Applications of Photonics, 1997
Laser scanning imaging techniques are a powerful tool in investigating microscopic processes with high resolution in three dimensions. A recent extension of this method by use of multiphoton excitation of fluorophores attached to cells under study is opening up many new areas of research in developmental biology, neurology and in the physiology of ...
openaire   +1 more source

Multiphoton-Excitation Fluorescence Microscopy and Membranes

2006
In the last few years fluorescence microscopy has become a very useful technique to study the lateral structure of membranes. Several experimental strategies using fluorescence microscopy techniques (epifluorescence, confocal and two-photon excitation) have appeared in the literature employing GUVs as model systems.
openaire   +2 more sources