Results 101 to 110 of about 3,074,988 (359)

Wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light [PDF]

open access: yes, 2014
The project was supported by the UK Engineering and Physical Sciences Research Council, RS MacDonald Charitable Trust, SULSA, and the St. Andrews 600th anniversary BRAINS appeal. K. D.
Dholakia, K.   +4 more
core   +2 more sources

A coiled‐coil domain triggers oligomerization of MmpL10, the mycobacterial transporter of trehalose polyphleate precursor

open access: yesFEBS Letters, EarlyView.
Most MmpLs transport lipids and glycolipids of the complex mycomembrane of mycobacteria. This study shows that MmpL10 from Mycobacterium smegmatis, the transporter of the trehalose polyphosphate precursor, harbors a coiled‐coil‐like extension. Biochemical and electron microscopy studies demonstrate that the coiled‐coil enables MmpL10 to trimerize.
Julie Couston   +7 more
wiley   +1 more source

Fluorescence microscopy shadow imaging for neuroscience

open access: yesFrontiers in Cellular Neuroscience
Fluorescence microscopy remains one of the single most widely applied experimental approaches in neuroscience and beyond and is continuously evolving to make it easier and more versatile.
V. V. G. Krishna Inavalli   +8 more
doaj   +1 more source

Bayesian-based deconvolution fluorescence microscopy using dynamically updated nonparametric nonstationary expectation estimates [PDF]

open access: yesarXiv, 2015
Fluorescence microscopy is widely used for the study of biological specimens. Deconvolution can significantly improve the resolution and contrast of images produced using fluorescence microscopy; in particular, Bayesian-based methods have become very popular in deconvolution fluorescence microscopy.
arxiv  

Adaptive Optimisation of Illumination Beam Profiles in Fluorescence Microscopy [PDF]

open access: yes, 2015
Wide-field fluorescence microscope techniques such as single/selective plane illumination microscope (SPIM) are typically configured to image large regions of a sample at once.
Girkin, J.M.   +4 more
core   +1 more source

Identification of functional and diverse circulating cancer‐associated fibroblasts in metastatic castration‐naïve prostate cancer patients

open access: yesMolecular Oncology, EarlyView.
Cancer‐associated fibroblasts (CAFs) promote cancer growth, invasion (metastasis), and drug resistance. Here, we identified functional and diverse circulating CAFs (cCAFs) in patients with metastatic prostate cancer (mPCa). cCAFs were found in higher numbers and were functional and diverse in mPCa patients versus healthy individuals, suggesting their ...
Richell Booijink   +6 more
wiley   +1 more source

Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index reconstruction [PDF]

open access: yes, 2017
Though structured illumination (SI) microscopy is a popular imaging technique conventionally associated with fluorescent super-resolution, recent works have suggested its applicability towards sub-diffraction coherent imaging with quantitative endogenous biological contrast.
arxiv   +1 more source

Microfabricated rubber microscope using soft solid immersion lenses [PDF]

open access: yes, 2006
We show here a technique of soft lithography to microfabricate efficient solid immersion lenses (SIL) out of rubber elastomers. The light collection efficiency of a lens system is described by its numerical aperture (NA), and is critical for applications
Gambin, Yann   +2 more
core   +1 more source

Single-molecule fluorescence microscopy review: shedding new light on old problems

open access: yesBioscience Reports, 2017
Fluorescence microscopy is an invaluable tool in the biosciences, a genuine workhorse technique offering exceptional contrast in conjunction with high specificity of labelling with relatively minimal perturbation to biological samples compared with many ...
Sviatlana Shashkova, M. Leake
semanticscholar   +1 more source

Inducible fluorescent speckle microscopy [PDF]

open access: yesJournal of Cell Biology, 2016
The understanding of cytoskeleton dynamics has benefited from the capacity to generate fluorescent fiducial marks on cytoskeleton components. Here we show that light-induced imprinting of three-dimensional (3D) fluorescent speckles significantly improves speckle signal and contrast relative to classic (random) fluorescent speckle microscopy. We predict
Helder Maiato   +5 more
openaire   +4 more sources

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