Results 121 to 130 of about 768,563 (305)

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Modern age of pathological sciences: Fluorescence microscopy for proficient treatment

In light microscopy, achieving uniform lighting throughout the field of view is essential to obtain clear images. However, thicker samples commonly suffer from image blur due to out-of-focus illumination, notably raised in fluorescence microscopy because
Mehmood, Muhammad Qasim, Zubair, Muhammad, Javed, Isma, Satti, Azhar Javed   +3 more
core   +1 more source

2-Photon Laser Scanning Microscopy on Native Human Cartilage

, 2005
Martini J, Tönsing K, Dickob M, Anselmetti D. 2-Photon Laser Scanning Microscopy on Native Human Cartilage. In: Proceedings of SPIE - The International Society for Optical Engineering. Vol 5860.
Michael Dickob, Martini, Jörg, Joerg Martini, Dickob, Michael, Katja Toensing, Dario Anselmetti, Tönsing, Katja, Anselmetti, Dario ; https://orcid.org/   +7 more
core   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Evaluation of combined LED-fluorescence microscopy and bleach sedimentation for diagnosis of tuberculosis at peripheral health service level.

, 2011
Sputum microscopy is the only diagnostic for tuberculosis (TB) available at peripheral levels of health service in resource-poor countries. Its sensitivity is reduced in high HIV-prevalence settings.
Gagnidze Laramie, Gagnidze, Laramie, Guerin, P J, Maryline Bonnet, Philippe J Guerin, Ramsay, A, Guerin, Philippe J, Ramsay Andrew, Bonnet, M, Gagnidze, L, Francis Varaine, Guerin Philippe J., Githui, W, Guerin, PJ, Githui Willie, Bonte, L, Bonte, Laurence, Varaine, Francis, Philippe J. Guerin, Bonte Laurence, Laramie Gagnidze, Bonnet, Maryline, Varaine, F, Varaine Francis, Willie Githui, Laurence Bonte, Andrew Ramsay, Guerin, Philippe, Ramsay, Andrew, Githui, Willie, Bonnet Maryline   +30 more
core   +1 more source

Fluorescence microscopy in the spotlight [PDF]

Microscopy Research and Technique, 2014
Francesca Cella, Zanacchi, Paolo, Bianchini, Giuseppe, Vicidomini   +2 more
openaire   +2 more sources

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source