Results 111 to 120 of about 768,563 (305)

Fluorescence microscopy on the biocompatibility of gentamicin-coated hydroxyapatite (HA) material on osteoblast [PDF]

, 2010
This study was carried out to observe the biocompatibility of gentamicin-coated hydroxyapatite (HA) on osteoblast using fluorescence microscopy. The specific objective was to observe the viability of the osteoblast on the gentamicin-coated hydroxyapatite
Othman, Fauziah, Zulkifly, Ahmad Hafiz, Moin, Saidi, Mustaffa, Rusnah, Idris, Besar, Besar, Idris, Che Seman, Che Nor Zarida, Goriman Khan, Mohd Azam Khan, Ahmad, Azfar Rizal, Wong, C. C.   +9 more
core  

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

HIGH-THROUGHPUT FLUORESCENCE MICROSCOPY FOR AUTOMATED CLINICAL APPLICATIONS [PDF]

, 2017
Fluorescence in situ hybridization (FISH) is a powerful tool for visualizing and detecting genetic abnormalities. Manual scoring FISH analysis is a tedious and labor-and-time-consuming task. Automated image acquisition and analysis provide an opportunity
Li, Zheng
core  

Monitoring photosynthesis in individual cells of Synechocytis sp. PCC 6803 on a picosecond timescale

, 2010
Picosecond fluorescence kinetics of wild-type (WT) and mutant cells of Synechocystis sp. PCC 6803, were studied at the ensemble level with a streak-camera and at the cell level using fluorescence-lifetime-imaging microscopy (FLIM).
S.P. Laptenok, van Amerongen, H., Laptenok, S., Amerongen, H., van, P Laptenok, S., H. van Amerongen, Krumova, Sb, Borst, J.W., Gombos, Z., Krumova, S.B., Z. Gombos, Krumova, S.K.B., B. Ughy, S.B. Krumova, Aijani, G., Ughy, B., Laptenok, S.P., G. Ajlani, W Borts, J., Ajlani, G., J.W. Borst   +20 more
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Performance of LED-Based Fluorescence Microscopy to Diagnose Tuberculosis in a Peripheral Health Centre in Nairobi.

, 2011
Sputum microscopy is the only tuberculosis (TB) diagnostic available at peripheral levels of care in resource limited countries. Its sensitivity is low, particularly in high HIV prevalence settings. Fluorescence microscopy (FM) can improve performance of
Gagnidze Laramie, Gagnidze, Laramie, Maryline Bonnet, Guérin, P J, Guérin, Philippe Jean, Ramsay, A, Ramsay Andrew, Bonnet, M, Gagnidze, L, Francis Varaine, Githui, W, Guérin Philippe Jean, Githui Willie, Bonte, L, Bonte, Laurence, Varaine, Francis, Bonte Laurence, Guérin, PJ, Laramie Gagnidze, Bonnet, Maryline, Varaine, F, Varaine Francis, Willie Githui, Laurence Bonte, Andrew Ramsay, Philippe Jean Guérin, Ramsay, Andrew, Githui, Willie, Bonnet Maryline   +28 more
core   +1 more source

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

Studying Protein Aggregation in the Context of Liquid-liquid Phase Separation Using Fluorescence and Atomic Force Microscopy, Fluorescence and Turbidity Assays, and FRAP. [PDF]

Bio Protoc, 2020
Babinchak WM, Surewicz WK.
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Fluorescence Lifetime Imaging Microscopy (FLIM) Data Analysis with TIMP

Fluorescence Lifetime Imaging Microscopy (FLIM) allows fluorescence lifetime images of biological objects to be collected at 250 nm spatial resolution and at (sub-)nanosecond temporal resolution.
Vladimir V. Apanasovich, Ivo H. M. van Stokkum, Antonie J. W. G. Visser, Jan Willem Borst, Katharine M. Mullen, Sergey Laptenok   +5 more
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