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Fluorescence tissue microscopy
Progress in Biophysics and Molecular Biology, 2022The increasing need to investigate large biological specimens like whole tissues in 3D has driven the development of novel optical techniques capable of volumetric imaging with no compromises in terms of field-of-view and spatial resolution. On the other hand, a major challenge in the imaging of bulk biological tissues is their strong scattering which ...
Sacconi, L +3 more
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Nature Methods, 2005
Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the underlying photophysical phenomena. Understanding the principles underlying fluorescence microscopy is useful when attempting to solve imaging problems.
Jeff W, Lichtman, José-Angel, Conchello
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Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the underlying photophysical phenomena. Understanding the principles underlying fluorescence microscopy is useful when attempting to solve imaging problems.
Jeff W, Lichtman, José-Angel, Conchello
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Fluorescent Speckle Microscopy
Cold Spring Harbor Protocols, 2011INTRODUCTIONFluorescent speckle microscopy (FSM) is a live imaging and quantitative measurement technique used for analyzing motion and turnover of macromolecular assemblies in vivo and in vitro. It differs from related imaging techniques such as photobleaching and photoactivation in its use of substantially lower concentrations of fluorescently ...
Lisa A, Cameron +2 more
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Factors in Fluorescence Microscopy
Journal of the Optical Society of America, 1947This paper is concerned with a presentation and an attempted evaluation of the various factors governing successful observations with the microscope using fluorescence technics. The primary considerations are: (1) brightness of the fluorescent image in absolute terms and (2) brightness level, image contrast and resolving power of the eye.
D H, HAMLY, C, SHEARD
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Fundamentals of Fluorescence and Fluorescence Microscopy
2003This chapter discusses the fundamental physics of fluorescence. The application of fluorescence to microscopy represents an important transition in the development of microscopy, particularly as it applies to biology. It enables quantitating the amounts of specific molecules within a cell, determining whether molecules are complexing on a molecular ...
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Abstract Self-luminous fluorophores allow us to stain with high contrast using multiple labels of high specificity and sensitivity. Similarly, fluorescent proteins allow sophisticated labelling of tissues, cells and sub-cellular components. Fluorescent proteins permit marking of proteins.
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Nature Methods, 2005
Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues.
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Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues.
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Fluorescence (Multiwave) Confocal Microscopy
Dermatologic Clinics, 2016In addition to reflectance confocal microscopy, multiwave confocal microscopes with different laser wavelengths in combination with exogenous fluorophores allow fluorescence mode confocal microscopy in vivo and ex vivo. Fluorescence mode confocal microscopy improves the contrast between the epithelium and the surrounding soft tissue and allows the ...
Welzel, Julia +2 more
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Digital imaging fluorescence microscopy
1986For digital imaging fluorescence microscopy we have used a Grinnell 274 image processor interfaced to a Lab Datex LSI 11/23 under RT-11 controlled by Integrated Solutions 68010 operating under Unix BSD 4.2. Experimental approaches to address (a) photobleaching of the fluorophore, (b) misalignment of images by nonparfocal microscope optics and (c) the ...
Smith, L.C. +3 more
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Introduction to Fluorescence Microscopy
2011This chapter is an overview of basic principles of fluorescence microscopy, including a brief history on the invention of this type of microscopy. The chapter highlights important points related to properties of fluorochromes, resolution in fluorescence microscopy, phase contrast and fluorescence, fluorescence filters, construction of a fluorescence ...
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