Results 121 to 130 of about 3,074,988 (359)
Calibrating evanescent-wave penetration depths for biological TIRF microscopy
, 2019 Roughly half of a cells proteins are located at or near the plasma membrane. In this restricted space the cell senses its environment, signals to its neighbors and ex-changes cargo through exo- and endocytotic mechanisms.
Becherer, Ute +4 more
core +1 more source
Proceedings of the National Academy of Sciences of the United States of America, 2005 Contrary to the well known diffraction limit, the fluorescence microscope is in principle capable of unlimited resolution. The necessary elements are spatially structured illumination light and a nonlinear dependence of the fluorescence emission rate on ...
M. Gustafsson
semanticscholar +1 more source
Molecular Oncology, EarlyView.The complex mode of action of the topoisomerase II inhibitor etoposide in triggering apoptosis involves several mechanisms: overexpression of the mitochondrial protein VDAC1, leading to its oligomerization and formation of a large channel that mediates the release of pro‐apoptotic protein; and overexpression of the apoptosis regulators p53, Bax, and ...
Aditya Karunanithi Nivedita +1 more
wiley +1 more source
Fluorescence microscopy: A tool to study autophagy
AIP Advances, 2015 Autophagy is a cellular recycling process through which a cell degrades old and damaged cellular components such as organelles and proteins and the degradation products are reused to provide energy and building blocks. Dysfunctional autophagy is reported
Shashank Rai, Ravi Manjithaya
doaj +1 more source
Molecular Oncology, EarlyView.NAD+ regeneration by mitochondrial complex I NADH dehydrogenase is important for cancer cell proliferation. Specifically, NAD+ is necessary for the activities of NAD+‐dependent deacetylases SIRT3 and SIRT7, which suppress the expression of p21Cip1 cyclin‐dependent kinase inhibitor, an antiproliferative molecule, at the translational and transcriptional
Masato Higurashi +5 more
wiley +1 more source
Far-field fluorescence microscopy beyond the diffraction limit: Fluorescence imaging with ultrahigh resolution [PDF]
arXiv, 2007 Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution has stimulated research into developing methods to image beyond the diffraction limit based on far-field ...
arxiv
Detectors for Fluorescence Microscopy
, 2001 The low light levels originating from living cells viewed in the fluorescence microscope place significant limitations on the spatial and temporal resolution which can be achieved. The development of intensified video cameras has enabled the detection, visualization and measurement of these low level signals.
openaire +4 more sources
Stochastic variation in the FOXM1 transcription program mediates replication stress tolerance
Molecular Oncology, EarlyView.Cellular heterogeneity is a major cause of drug resistance in cancer. Segeren et al. used single‐cell transcriptomics to investigate gene expression events that correlate with sensitivity to the DNA‐damaging drugs gemcitabine and prexasertib. They show that dampened expression of transcription factor FOXM1 and its target genes protected cells against ...
Hendrika A. Segeren +4 more
wiley +1 more source
, 2007 Highly ordered periodic arrays of silver nanoparticles have been fabricated which exhibit surface plasmon resonances in the visible spectrum. We demonstrate the ability of these structures to alter the fluorescence properties of vicinal dye molecules by ...
Cade, N. I. +2 more
core +1 more source
Open-source do-it-yourself multi-color fluorescence smartphone microscopy.
Biomedical Optics Express, 2017 Fluorescence microscopy is an important technique for cellular and microbiological investigations. Translating this technique onto a smartphone can enable particularly powerful applications such as on-site analysis, on-demand monitoring, and point-of ...
Yulung Sung +2 more
semanticscholar +1 more source