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PCIB an Antiauxin Enhances Microspore Embryogenesis in Microspore Culture of Brassica juncea
Plant Cell, Tissue and Organ Culture, 2006An efficient protocol to improve microspore embryogenesis is established in an important oleiferous crop, Brassica juncea (Indian mustard). Colchicine was used for enhancing microspore embryogenesis and also to obtain doubled haploid embryos. Colchicine at high concentrations (>10 mg l−1), for 24 h, proved convenient for direct recovery of diploid ...
Agarwal, P.K. +4 more
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1994
The maize pollen grain represents the beginning of a short-lived gametophytic phase during which the two sperm are delivered to the embryo sac prior to fertilization. Although this stage of the life cycle normally consists of only a few cell divisions, under certain experimental conditions, gametophytes can be induced to undergo an altered development,
J. F. Petolino, A. D. Genovesi
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The maize pollen grain represents the beginning of a short-lived gametophytic phase during which the two sperm are delivered to the embryo sac prior to fertilization. Although this stage of the life cycle normally consists of only a few cell divisions, under certain experimental conditions, gametophytes can be induced to undergo an altered development,
J. F. Petolino, A. D. Genovesi
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Nuclear fusion in cultured microspores of barley
Plant Cell Reports, 1987Fusion of the generative and vegetative nuclei physically separated by a wall has been observed in cultured microspores of barley. The generative cell appears to play an active role in fusion as it elongates toward the vegetative nucleus, becomes detached from the microspore wall, and finally completely encloses the vegetative nucleus.
Lee, Feng-Ming, Chen, Chi-Chang
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Albinism in Microspore Culture
2008Frequent formation of albino plants from in vitro cultured microspores is a particular problem for chromosome doubled haploid production in cereals and grasses. The phenomenon was first thought to be associated with maternal inheritance of plastids visualized by large deletions and rearrangements of plastid genomes in albino plants.
A. M. Torp, S. B. Andersen
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2003
Rye (Secale cereale L.) is an important cereal in Europe, with approximately 90% of global rye production concentrated in this area. In subtropical low latitude areas, rye is grown primarily as a late-fall, early-spring forage and, secondarily, for grain.
S. Pulli, Y.-D. Guo
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Rye (Secale cereale L.) is an important cereal in Europe, with approximately 90% of global rye production concentrated in this area. In subtropical low latitude areas, rye is grown primarily as a late-fall, early-spring forage and, secondarily, for grain.
S. Pulli, Y.-D. Guo
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Microspore Culture in Brassica
2003Pioneering research in Brassica microspore culture (1,2,3)rapidly led to the realization that microspores provide a powerful alternative to protoplast culture as a single-celled culture method in plants. These two single-celled systems are fundamentally different, both in tissue origin and in genetic variability.
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Brassica Improvement Through Microspore Culture
2004The microspore culture system developed for Brassica species represents one of the most efficient systems available for generating microspore-derived embryos and double haploid plants. Haploid embryos from many Brassica species can be produced at frequencies suitable for both basic research and practical applications.
Ferrie, Alison, Keller, Wilfred
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Isolated wheat microspore culture
Plant Cell, Tissue and Organ Culture, 1995The use of doubled haploid plants in a wheat breeding program requires an efficient haploid production system. While the techniques for producing doubled haploids from anther culture are well established, those for isolated microspores are complicated and inefficient.
Vicki D. Gustafson +4 more
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Asparagus microspore and anther culture
2003Asparagus is a dioecious, perennial crop where male plants generally yield more than female plants. Consequently, an all-male hybrid can have improved productivity compared to one that is dioecious. Genotypes of males and females are Mm and mm, respectively, and all-male hybrids result from crossing females and supermales (MM).
D. J. Wolyn, B. Nichols
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