Results 131 to 140 of about 1,588 (172)

Cytological and ultrastructural changes induced in anther and isolated-microspore cultures in barley: Fe deposits in isolated-microspore cultures

Journal of Structural Biology, 2005
To gain further insight into the role played by sporophytic anther tissues in the early stages of the androgenic process, we have compared the cytology and ultrastructure of barley embryogenic pollen grains obtained by anther culture with those obtained by isolated-microspore culture.
A, Pulido, F, Bakos, A, Castillo, M P, Vallés, B, Barnabas, A, Olmedilla   +5 more
openaire   +2 more sources

Anther and microspore culture in tobacco

2003
Serving as the oldest model species, several variants of tobacco anther and microspore culture have been developed over the years, depending on the timing of the inductive stress treatment and microspore isolation (Heberle-Bors et al., 1996). A convenient, one-step procedure of anther culture in Nicotiana tabacum consists of a cold-treatment of excised
A. Touraev, E. Heberle-Bors
openaire   +1 more source

Isolated Microspore Culture of Maize

1994
The process of androgenesis is unique, in that haploid embryos arise from single gametophytic cells. Clear evidence for the unicellular origin of somatic embryos is lacking for most regeneration systems (Fransz and Schel 1991); therefore, totipotent microspores offer a potentially useful subject for both basic and applied studies.
S. M. Pescitelli, C. D. Johnson, J. F. Petolino   +2 more
openaire   +1 more source

Anther culture and isolated microspore culture in timothy

2003
Timothy (Phleum pratense L.) is one of the basic forage grasses in the world and the most important grass in winter snow covered northern latitudes. It is considered to be allohexaploid in nature (2n=6x=42). Timothy is valued for its winter hardiness, good palatability, and moderate feeding quality.
S. Pulli, Y.-D. Guo
openaire   +1 more source

Microspore culture of Brassica species

2003
The production of haploid plants from isolated microspores of Brassica napus (L.) was first reported by Lichter in 1982 (Lichter, 1982). The embryo yields from these initial experiments were low but have since increased through manipulation of the culture technique.
openaire   +2 more sources

Microspore Culture for Haploid Plant Production

1995
Haploid plants can be produced through in vitro culture of male gametophytic cells at the microspore or immature pollen developmental stage. These cells respond in vitro by undergoing embryogenesis or haploid callus proliferation. Plants can be regenerated through shoot and root induction in haploid callus and through culture of haploid embryos.
A. M. R. Ferrie, W. A. Keller
openaire   +1 more source

Culture of Isolated Microspores of Saccharum spontaneum)

Zeitschrift für Pflanzenphysiologie, 1984
Summary Microspore culture of Saccharum spontaneum L. was investigated as a means to produce haploid plants. Isolated and cultured microspores initiated sporophytic development which in 4 to 6 weeks resulted in procalli and calli. The percentage of microspores producing multicellular procalli was increased by removing the nonviable microspores ...
Maud A.W. Hinchee, Maureen M.M. Fitch
openaire   +1 more source

Embryogenesis in microspore culture of Vitis subspecies

2015
VITIS - Journal of Grapevine Research, Vol. 36 No.
Sefc, Kristina M., Ruckenbauer, P., Regner, F.   +2 more
openaire   +1 more source

Protocol for microspore culture in Brassica

2003
We have been using microspore culture of Brassica at the Institute of Horticulture and Crop Sciences, Agricultural University of Norway, since 1989, our focus has been on Brassica oleracea (cabbage), Brassica napus (swede) and Brassica rapa (chinese cabbage and summer turnip rapeseed). The protocol presented here is based on over 12 years of experience.
openaire   +1 more source

Protocol for broccoli microspore culture

2003
Broccoli (Brassica oleracea var. italica) is a vegetable cole crop of considerable interest to breeders and seed companies since its area of production has increased in recent years mainly due to improved nutritional qualities, e.g. anticancer properties. Breeding companies strive more and more to bring F1 hybrid seeds onto the market.
openaire   +1 more source