Results 141 to 150 of about 3,834,083 (198)

Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production

open access: yesPlant Cell, Tissue and Organ Culture, 2010
An isolated microspore culture provides an excellent system for the study of microspore induction and embryogenesis, provides a platform for an ever-increasing array of molecular studies, and can produce doubled haploid (DH) plants, which are used to ...
Alison M R Ferrie
exaly   +2 more sources

Cereal microspore culture

Plant Science, 1995
The present review will examine some of the recent developments in cereal microspore culture. Experimental procedures as well as applied purposes will be considered. This article will mainly concentrate on the culture of isolated microspores, but due to the close relationship some aspects of anther culture will be discussed also.
Horst Lorz, Alwine Jähne
exaly   +2 more sources

Barley Isolated Microspore Culture

2021
The production of doubled haploids (DHs) has proved to be a highly valuable tool to obtain new cultivars. Among the cereals, barley (Hordeum vulgare L.) is the most successful species in large-scale haploid production. Techniques employed for this purpose are based on either the gynogenetic or the androgenetic pathway.
Luís, Cistué, Begoña, Echávarri
openaire   +2 more sources

Microspore culture technology

Journal of Tissue Culture Methods, 1989
Methodologies of microspore culture that have been successfully developed in Brassica napus, Zea mays, and Hordeum vulgare, three major, important crop species. However, the protocols can be applied to other plant species effectively, perhaps with some testing and ...
Bin Huang, Wilfred A. Keller
openaire   +1 more source

Isolated Microspore Culture in Brassica napus

2020
Isolated microspore culture is the most efficient technique among those used to induce microspore embryogenesis. In the particular case of Brassica napus, it is also the most widely used and optimized. In this chapter, we describe a protocol for microspore culture in B.
Patricia, Corral-Martínez   +2 more
openaire   +2 more sources

Microspore development in cultured maize anthers

Plant Cell Reports, 1988
The present study follows in vivo and in vitro microspore development utilizing an anther culture-responsive maize genotype (Pa91×FR16) and a DNA-specific fluorescent dye (mithramycin). Cultured anthers were sampled at various times and scored for abnormal microspore divisions, multicellular masses, and embryo-like structures. The frequency of abnormal
S M, Pescitelli, J F, Petolino
openaire   +2 more sources

Anther and Microspore Culture

1994
The maize pollen grain represents the beginning of a short-lived gametophytic phase during which the two sperm are delivered to the embryo sac prior to fertilization. Although this stage of the life cycle normally consists of only a few cell divisions, under certain experimental conditions, gametophytes can be induced to undergo an altered development,
J. F. Petolino, A. D. Genovesi
openaire   +1 more source

Albinism in Microspore Culture

2008
Frequent formation of albino plants from in vitro cultured microspores is a particular problem for chromosome doubled haploid production in cereals and grasses. The phenomenon was first thought to be associated with maternal inheritance of plastids visualized by large deletions and rearrangements of plastid genomes in albino plants.
A. M. Torp, S. B. Andersen
openaire   +1 more source

Nuclear fusion in cultured microspores of barley

Plant Cell Reports, 1987
Fusion of the generative and vegetative nuclei physically separated by a wall has been observed in cultured microspores of barley. The generative cell appears to play an active role in fusion as it elongates toward the vegetative nucleus, becomes detached from the microspore wall, and finally completely encloses the vegetative nucleus.
Lee, Feng-Ming, Chen, Chi-Chang
openaire   +2 more sources

Microspore culture of rye

2003
Rye (Secale cereale L.) is an important cereal in Europe, with approximately 90% of global rye production concentrated in this area. In subtropical low latitude areas, rye is grown primarily as a late-fall, early-spring forage and, secondarily, for grain.
S. Pulli, Y.-D. Guo
openaire   +1 more source

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