ABSTRACT Cdt1 is a mixed folded protein critical for DNA replication licensing and it also has a “moonlighting” role at the kinetochore via direct binding to microtubules and the Ndc80 complex. However, it is unknown how the structure and conformations of Cdt1 could allow it to participate in these multiple, unique sets of protein complexes.
Kyle P. Smith+9 more
wiley +1 more source
Correction to Supporting Information for Chakraborty et al., Cryo-ET suggests tubulin chaperones form a subset of microtubule lumenal particles with a role in maintaining neuronal microtubules. [PDF]
europepmc +1 more source
CITK modulates BRCA1 recruitment at DNA double strand breaks sites through HDAC6. [PDF]
Iegiani G+9 more
europepmc +1 more source
Characterization of microtubule protein oligomers by analytical ultracentrifugation.
J.M. Marcum, Gary G. Borisy
openalex +1 more source
Phase separation of the poplar microtubule-associated protein PagPCaP1a aids microtubule depolymerization in response to high salt. [PDF]
Lian N+9 more
europepmc +1 more source
TRiC Is a Structural Component of Mammalian Sperm Axonemes
ABSTRACT The TRiC chaperonin is responsible for folding ~5%–10% of the proteome in eukaryotic cells. Our recent cryo‐electron microscopy studies of axonemes from diverse mammalian cell types led to the surprising discovery that a fully assembled TRiC chaperonin is a structural component of mammalian sperm flagella, where it is tethered to the radial ...
Alan Brown+3 more
wiley +1 more source
Electrochemically modulated single-molecule localization microscopy for <i>in vitro</i> imaging cytoskeletal protein structures. [PDF]
Lei C, Hu D.
europepmc +1 more source
Phosphorylation at the Helm: Kinase‐Mediated Regulation of Primary Cilia Assembly and Disassembly
ABSTRACT The primary cilium serves as an antenna of most vertebrate cells and is important for conveying cues from several signaling pathways into appropriate cellular responses during development and homeostasis. Cilia assembly and disassembly processes are thought to be strictly controlled; however, the precise nature of molecular events underlying ...
Andrea Lacigová, Lukáš Čajánek
wiley +1 more source
Microtubule-modulating drugs alter sensitivity to isoflurane in mice. [PDF]
Li N+17 more
europepmc +1 more source