Results 31 to 40 of about 6,179,746 (234)

辣木籽凝乳酶的提取分离条件优化Optimization of extraction and separation conditions of milk-clotting protease from Moringa oleifera seeds

Zhongguo youzhi, 2023
为给辣木籽凝乳酶的开发利用提供理论依据，以脱脂辣木籽粉为原料，采用盐法提取、硫酸铵（AS）分级沉淀和离子交换色谱法提取分离凝乳酶。通过单因素试验和正交试验对辣木籽凝乳酶的盐法提取条件进行优化，采用单因素试验对离子交换色谱法分离纯化条件进行优化，并采用SDS-PAGE和RP-HPLC对分离得到的辣木籽凝乳酶的分子质量和纯度进行分析。结果表明：盐法提取的最佳条件为料液比1∶ 10、提取温度30 ℃、pH 7.15、NaCl浓度0.3 mol/L、提取时间0.5 h；在20%~40 ...
吴改转1，庄玺1，王雪峰1,2，黄艾祥1WU Gaizhuan1, ZHUANG Xi1, WANG Xuefeng1,2, HUANG Aixiang1
doaj   +1 more source

THE INFLUENCE OF MILK-CLOTTING ENZYMES ON THE FUNCTIONAL PROPERTIES OF PIZZA-CHEESES

Пищевые системы, 2020
The effect of the type and dose of milk-clotting enzymes (Chy-max® M based on recombinant camel chymosin, Fromase® TL based on Rhizomucor miehei protease) on the physicochemical, functional properties and shelf life of pizza-cheeses was studied.
D. S. Myagkonosov, V. A. Mordvinova, I. N. Delitskaya, D. V. Abramov, E. G. Ovchinnikova   +4 more
doaj   +1 more source

Clotting Properties of Onopordum tauricum (Willd.) Aqueous Extract in Milk of Different Species

Foods, 2020
Plant proteases used in cheesemaking are easily available and could increase the acceptability of cheeses, otherwise hindered by ethical issues (e.g., religions, dietary habits, aversion to genetically engineered food and food ingredients).
Massimo Mozzon, Roberta Foligni, Cinzia Mannozzi, Federica Zamporlini, Nadia Raffaelli, Lucia Aquilanti   +5 more
doaj   +1 more source

The influence of milk-clotting enzymes on the lipid composition and organoleptic properties of semi-matured cheeses

Biotecnología en el Sector Agropecuario y Agroindustrial, 2021
The milk-clotting enzymes used in the production of semi-matured and matured cheeses, can influence the metabolic processes that occur during maturation.
Angélica María García Torres, Karen Gineth Usgame Fagua, Carlos Iván Rojas Morales, Oscar Julio Medina Vargas   +3 more
doaj   +1 more source

Caseinolytic and milk-clotting activities from Moringa oleifera flowers [PDF]

, 2012
This work reports the detection and characterization of caseinolytic and milk-clotting activities from Moringa oleifera flowers. Proteins extracted from flowers were precipitated with 60% ammonium sulphate.
Napoleão, Thiago H., Pontual, Emmanuel V., Carvalho, Belany E.A., Bezerra, Ranilson S., Coelho, Luana C.B.B., Paiva, Patrícia M.G.   +5 more
core   +1 more source

METALLOPROTEASE FROM THE CULTURAL LIQUID OF Pleurotus osreatus [PDF]

Biotechnologia Acta, 2019
The aim of this work was to identify and to study physical and chemical properties of the enzyme preparation which was obtained from the cultural liquid of Pleurotus ostreatus.
Sakovich V. V., Stohniy Ye. M., Zhernossekov D. D., Rebriev A. V., Korolova D. S., Marunych R. Yu., Chernyshenko V. O.   +6 more
doaj   +1 more source

A novel quinazolinone insulin receptor inhibitor and its synergy with an EGFR inhibitor in glucose‐driven glioblastoma

Molecular Oncology, EarlyView.
The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka, Wioleta Cieślik, Wojciech Płaziński, Katarzyna Stępnik, Anna Boguszewska‐Czubara, Elżbieta Kot, Robert Musioł, Mateusz Jasica, Anna Mrozek‐Wilczkiewicz, Katarzyna Malarz   +9 more
wiley   +1 more source

Integrative Multi‐Omics Analysis Reveals a Mitochondrial–Immune Axis Associated With Neoadjuvant Chemotherapy Response in High‐Grade Serous Ovarian Cancer

Advanced Science, EarlyView.
Integrative multi‐omics analysis delineates a mitochondrial–immune axis governing neoadjuvant chemotherapy response in high‐grade serous ovarian cancer. Immune‐active tumors exhibit enhanced B‐cell infiltration and favorable sensitivity, whereas metabolically rewired tumors display oxidative phosphorylation dependency and resistance.
Wei Jiang, Yi Wang, Xiaohang Lu, Qianlan Yao, Libing Xiang, Xu Cai, Xiaoyu Tu, Shaoxian Tang, Ying Xu, Yufan Cheng, Liming Shen, Huijuan Yang   +11 more
wiley   +1 more source

Lactiplantibacillus Plantarum GUANKE Alleviates Allergic Asthma by Restricting Monocyte‐to‐DC Differentiation Via Microbiota‐Associated IAA

Advanced Science, EarlyView.
Lactiplantibacillus plantarum GUANKE remodels the indigenous gut microbiota and is associated with microbiota‐dependent restoration of circulating IAA. IAA treatment attenuates JAK3‐STAT3 and Jag1‐Notch4‐Hey1 signaling and reduces CX3CR1+Ly6C− monocytic precursor generation and pulmonary CD11b+ DC accumulation, together with reduced Th2 inflammation ...
Yujia He, Jielan Mi, Zhiping Zhao, Xiao Zhang, Zhihan Yang, Kun Yue, Yuanming Huang, Liqiong Song, Xiaoting Wen, Jianguo Xu, Zhihong Ren   +10 more
wiley   +1 more source

High Pressure Homogenization of Porcine Pepsin Protease: Effects on Enzyme Activity, Stability, Milk Coagulation Profile and Gel Development. [PDF]

PLoS ONE, 2015
This study investigated the effect of high pressure homogenization (HPH) (up to 190 MPa) on porcine pepsin (proteolytic and milk-clotting activities), and the consequences of using the processed enzyme in milk coagulation and gel formation (rheological ...
Bruno Ricardo de Castro Leite Júnior, Alline Artigiani Lima Tribst, Marcelo Cristianini   +2 more
doaj   +1 more source