Results 201 to 210 of about 14,380 (242)

Single cell proteomic analysis defines discrete neutrophil functional states in human glioblastoma

Sadiku P, Brenes AJ, Mayer RL, Reyes L, Coelho P, van Stralen G, Zhang A, Sanchez-Garcia MA, Watts ER, Liaquat I, Howden AJ, Adekoya I, Boldbaatar A, MacRaild A, Risbridger S, Morrison GM, MacPherson H, Bruce C, Johnston S, Grecian R, Murphy FA, Pollard SM, Brennan PM, Mechtler K, Walmsley SR.   +24 more
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Engineered minichromosomes in plants

Experimental Cell Research, 2020
Artificial chromosome platforms are described in plants. Because the function of centromeres is largely epigenetic, attempts to produce artificial chromosomes with plant centromere DNA have failed. The removal of the centromeric sequences from the cell strips off the centromeric histone that is the apparent biochemical marker of centromere activity ...
James A, Birchler, Nathan C, Swyers
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Engineered Plant Minichromosomes

2010
The advent of transgenic technologies has met many challenges, both technical and political; however, these technologies are now widely applied, particularly for crop improvement. Bioengineering has resulted in plants carrying resistance to herbicides, insects, and viruses, as well as entire biosynthetic pathways.
Robert T, Gaeta, Lakshminarasimhan, Krishnaswamy   +1 more
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Engineered minichromosomes in plants

Current Opinion in Biotechnology, 2007
Genetic engineering for complex or combined traits requires the simultaneous expression of multiple genes, and has been considered as the bottleneck for the next generation of genetic engineering in plants. Minichromosome technology provides one solution to the stable expression and maintenance of multiple transgenes in one genome.
Weichang, Yu, Fangpu, Han, James A, Birchler   +2 more
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Topological Complexity of SV40 Minichromosomes

Journal of Molecular Biology, 1996
During attempts to measure the extent to which the proteins of simian virus 40 (SV40) minichromosomes restrain the ability of SV40 DNA to alter its twist in response to temperature changes, we found that temperature-shift-induced linking number changes are not reversible for isolated minichromosomes, suggesting that such changes, both in isolated ...
R M, Givens, R A, Saavedra, J A, Huberman   +2 more
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Multiple maize minichromosomes in meiosis

Chromosome Research, 2012
In this study, four distinct minichromosomes derived from the maize B chromosome, were increased in copy number using the B chromosome's accumulation mechanism, namely nondisjunction at the second pollen mitosis and preferential fertilization of the egg.
Rick E, Masonbrink, Robert T, Gaeta, James A, Birchler   +2 more
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Form follows function in geminiviral minichromosome architecture.

Virus Research, 2015
A comprehensive survey on the viral minichromosomes of the begomoviruses Abutilon mosaic virus, tomato yellow leaf curl Sardinia virus, African cassava mosaic virus, Indian cassava mosaic virus (family Geminiviridae) during the course of infections in Nicotiana benthamiana is summarized.
T. Paprotka, Kathrin Deuschle, M. Pilartz, H. Jeske   +3 more
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Replication of SV40 minichromosomes in vitro

Chromosoma, 1992
We operationally define two forms of SV40 minichromosomes, a 75S-form, prepared at low salt concentration, referred to as native minichromosomes, and a 50S-form, obtained after treatment with 0.5 M potassium acetate, the salt-treated minichromosomes. Both preparations of minichromosomes serve well as templates for replication in vitro. Their respective
T, Krude, R, Knippers
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Engineered Minichromosomes in Plants

Critical Reviews in Plant Sciences, 2010
Engineered minichromosomes provide the ability to target transgenes to a defined insertion position for predictable expression on an independent chromosome. This technology promises to provide a means to add many genes to a synthetic chromosome in sequential manner.
James A. Birchler, Lakshminarasimhan Krishnaswamy, Robert T. Gaeta, Rick E. Masonbrink, Changzeng Zhao   +4 more
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