Results 31 to 40 of about 14,380 (242)

Variation of mitochondrial minichromosome composition in Hoplopleura lice (Phthiraptera: Hoplopleuridae) from rats

Parasites & Vectors, 2020
The family Hoplopleuridae contains at least 183 species of blood-sucking lice, which widely parasitize both mice and rats. Fragmented mitochondrial (mt) genomes have been reported in two rat lice (Hoplopleura kitti and H. akanezumi) from this family, but
Yi-Tian Fu, Yu Nie, De-Yong Duan, Guo-Hua Liu   +3 more
semanticscholar   +1 more source

Recollections of a Helmstetter Disciple

Life, 2023
Nearly fifty years ago, it became possible to construct E. coli minichromosomes using recombinant DNA technology. These very small replicons, comprising the unique replication origin of the chromosome oriC coupled to a drug resistance marker, provided ...
Alan C. Leonard
doaj   +1 more source

Bi-allelic MCM10 variants associated with immune dysfunction and cardiomyopathy cause telomere shortening

Nature Communications, 2021
Minichromosome maintenance protein 10 (MCM10) is critical for eukaryotic DNA replication. Here, by modelling MCM10 variants in human cell lines, the authors reveal a mechanism of MCM10-associated disease, finding that loss of MCM10 function constrains ...
Ryan M. Baxley, Wendy Leung, Megan M. Schmit, Jacob Peter Matson, Lulu Yin, Marissa K. Oram, Liangjun Wang, John Taylor, Jack Hedberg, Colette B. Rogers, Adam J. Harvey, Debashree Basu, Jenny C. Taylor, Alistair T. Pagnamenta, Helene Dreau, Jude Craft, Elizabeth Ormondroyd, Hugh Watkins, Eric A. Hendrickson, Emily M. Mace, Jordan S. Orange, Hideki Aihara, Grant S. Stewart, Edward Blair, Jeanette Gowen Cook, Anja-Katrin Bielinsky   +25 more
doaj   +1 more source

CRISPR-Cas9 Targeting of Hepatitis B Virus Covalently Closed Circular DNA Generates Transcriptionally Active Episomal Variants

mBio, 2022
Chronic hepatitis B virus (HBV) infection persists due to the lack of therapies that effectively target the HBV covalently closed circular DNA (cccDNA). We used HBV-specific guide RNAs (gRNAs) and CRISPR-Cas9 and determined the fate of cccDNA after gene ...
Maria Guadalupe Martinez, Emmanuel Combe, Aurore Inchauspe, Philippe Emmanuel Mangeot, Elodie Delberghe, Fleur Chapus, Gregory Neveu, Antoine Alam, Kara Carter, Barbara Testoni, Fabien Zoulim   +10 more
doaj   +1 more source

Polyoma virus minichromosomes: associated enzyme activities [PDF]

Journal of Virology, 1981
Polyoma minichromosomes were isolated and fractionated on glycerol gradients as described by Gourlie et al. (J. Virol. 38:805-814, 1981). Specific assays for DNa polymerases alpha, beta, and gamma, DNA topoisomerase I, and RNase H were carried out on each fraction.
Brian B. Gourlie, Vincent Pigiet, Carol Breaux, Marc R. Krauss, Charles R. King, Robert M. Benbow   +5 more
openalex   +4 more sources

The Chromatin Landscape of Kaposi’s Sarcoma-Associated Herpesvirus

Viruses, 2013
Kaposi’s sarcoma-associated herpesvirus is an oncogenic γ-herpesvirus that causes latent infection in humans. In cells, the viral genome adopts a highly organized chromatin structure, which is controlled by a wide variety of cellular and viral chromatin ...
Zsolt Toth, Kevin Brulois, Jae U. Jung
doaj   +1 more source

License to Replicate: Mechanisms of Licensing Eukaryotic Origins for DNA Replication. [PDF]

Bioessays
Origin licensing constitutes the first step in initiating eukaryotic genome replication. Licensed origins are marked by an MCM double hexamer complex. This article reviews the molecular mechanisms of MCM double hexamer loading in yeast and higher eukaryotes, based on insights gained from biochemical reconstitution and structural analyses.
Frisbie V, Bleichert F.
europepmc   +2 more sources

Epigenetic Analysis of SV40 Minichromosomes [PDF]

Current Protocols in Microbiology, 2017
AbstractSimian virus 40 (SV40) is one of the best‐characterized members of the polyomavirus family of small DNA tumor viruses. It has a small genome of 5243 bp and utilizes cellular proteins for its molecular biology, with the exception of the T‐antigen protein, which is coded by the virus and is involved in regulating transcription and directing ...
Balakrishnan, Lata, Milavetz, Barry
openaire   +2 more sources

Histone Modifications in Papillomavirus Virion Minichromosomes

mBio, 2021
A relatively unique feature of papillomaviruses is that the viral genome is associated with host histones inside the virion. However, little is known about the nature of the epigenome within papillomavirions or its biological relevance to the infectious viral cycle.
Samuel S. Porter, Jennifer C. Liddle, Kristen Browne, Diana V. Pastrana, Benjamin A. Garcia, Christopher B. Buck, Matthew D. Weitzman, Alison A. McBride   +7 more
openaire   +3 more sources

HAT1 signaling confers to assembly and epigenetic regulation of HBV cccDNA minichromosome

Theranostics, 2019
Rationale: Hepatitis B virus (HBV) is a leading cause of liver diseases. HBV covalently closed circular DNA (cccDNA) is a critical obstacle of complete elimination by anti-HBV therapy.
Guang Yang, Jinyan Feng, Yun-xia Liu, Man Zhao, Ying Yuan, Hongfeng Yuan, H. Yun, Ming-ming Sun, Y. Bu, Lei Liu, Zixian Liu, J. Niu, Ming Yin, Xijun Song, Z. Miao, Zhongqing Lin, Xiao-dong Zhang   +16 more
semanticscholar   +1 more source