Results 151 to 160 of about 145,034 (286)
Functional Ecology, EarlyView.Read the free Plain Language Summary for this article on the Journal blog. Abstract Industrial Haber–Bosch provides 32 Tg nitrogen (N) per year to global croplands. Such large amounts of N fertilization will reshape soil N cycles. One uncertainty is whether and how these long‐term N inputs impact soil biological N2 fixation (BNF) associated with plant ...
Xinyue Hu +10 more
wiley +1 more source
Dietary resilience of coral reef fishes to habitat degradation
Journal of Animal Ecology, EarlyView.Metabarcoding of gut contents shows that two common benthic‐feeding reef fishes with different feeding stratgies—a butterflyfish (Chaetodon capistratus) and a hamlet (Hypoplectrus puella)—shift diets on degraded reefs. These shifts mirror contrasting patterns in body condition: butterflyfish showed strong individual variation, whereas condition was ...
Friederike Clever +9 more
wiley +1 more source
Is the endophyte‐based plant protection against aphids mediated by changes in the insect microbiome?
Insect Science, EarlyView.We showed that the fungal endophyte Epichloë strain AR37 increased the plant resistance against aphids via the production of indole diterpene alkaloids. The reduction in aphid performance was not associated with changes in abundance/composition/diversity of the insect's bacterial microbiota, but additional endophyte effects on this microbiota cannot be
Daniel A. Bastías +5 more
wiley +1 more source
DPMAS: pipeline for 450K top table replication with MiSeq amplicons
, 2015 DNA methylation is one of the most important epigenetic modifications in gene expression regulation and it plays a crucial role in multiple biological processes. Therefore, DNA methylation profiling is important to reveal regions of the genome that are altered during development or that are perturbed by disease.
Perez-Gil, Daniel +7 more
openaire +1 more source
Insect Science, EarlyView.This study investigated the dietary composition of Sycanus bifidus, a generalist predator of the assassin bug that is artificially released into citrus orchards, and uncovered its trophic structure across various pest species using metabarcoding‐based molecular gut content analysis.
Weidong Huang +9 more
wiley +1 more source
Blubber Thickening Driven by UCP1 Inactivation: Insights from a Cetacean‐Like Transgenic Mouse Model
Integrative Zoology, EarlyView.UCP1 inactivation of cetaceans in mice drives BAT whitening and iWAT hyperplasia, promoting fat accumulation for aquatic adaptation. Abstract Cetaceans possess thick blubber, a specialized adipose tissue essential for thermal insulation, a streamlined body form, energy storage, and buoyancy. However, the mechanisms that underpin this adaptation are not
Qian Zhang +5 more
wiley +1 more source
GenomeTrakr WGS Protocol Collection and Workflow for MiSeq v2
Here we have created a collection of all the protocols used for WGS using the MiSeq, in order, from sample extraction to NCBI submission. This collection has three sections: Section 1: WGS Wet lab workflow for Illumina MiSeq Section 2: Dry lab workflow for sequence QC and NCBI submission - Direct Submission Section 3: Dry lab workflow for sequence ...
Tina Pfefer +4 more
openaire +1 more source
Integrative Zoology, EarlyView.Avian haemosporidian blood parasites are typically identified through Sanger sequencing of a partial cytochrome b fragment, the MalAvi barcoding region. Next‐generation sequencing is seldom used for avian blood parasite identification; this study demonstrates a higher detection rate of co‐infections via metabarcoding and its possible implications ...
Peter Pibaque +9 more
wiley +1 more source
Presumed TP53 mosaicism: variants detected using a NGS hereditary cancer multigene panel [PDF]
, 2019 Aims/Context: NGS multigene panels are routinely used to identify germline pathogenic variants in cancer susceptibility genes. In addition, NGS allows the identification of low-level mosaicism events that may not be detectable by conventional Sanger ...
Gonçalves, João +5 more
core
illumina MiSeq Dual Index Amplicon Sequencing Sample Preparation v1
, 2019 Preparation of PCR products for amplicon sequencing
openaire +1 more source