Results 151 to 160 of about 147,075 (286)
Insect Science, EarlyView.This study investigated the dietary composition of Sycanus bifidus, a generalist predator of the assassin bug that is artificially released into citrus orchards, and uncovered its trophic structure across various pest species using metabarcoding‐based molecular gut content analysis.
Weidong Huang +9 more
wiley +1 more source
DPMAS: pipeline for 450K top table replication with MiSeq amplicons
, 2015 DNA methylation is one of the most important epigenetic modifications in gene expression regulation and it plays a crucial role in multiple biological processes. Therefore, DNA methylation profiling is important to reveal regions of the genome that are altered during development or that are perturbed by disease.
Perez-Gil, Daniel +7 more
openaire +1 more source
Effect of the microbiome on pathogen susceptibility across four Drosophilidae species
Insect Science, EarlyView.Four Drosophilidae species were used to investigate how variation in the host microbiome influences susceptibility to infection. Microbial composition and abundance differed among species and treatments. The effects of microbiome manipulation on host survival were both species‐ and pathogen‐specific.
Hongbo Sun, Ben Longdon, Ben Raymond
wiley +1 more source
Blubber Thickening Driven by UCP1 Inactivation: Insights from a Cetacean‐Like Transgenic Mouse Model
Integrative Zoology, EarlyView.UCP1 inactivation of cetaceans in mice drives BAT whitening and iWAT hyperplasia, promoting fat accumulation for aquatic adaptation. Abstract Cetaceans possess thick blubber, a specialized adipose tissue essential for thermal insulation, a streamlined body form, energy storage, and buoyancy. However, the mechanisms that underpin this adaptation are not
Qian Zhang +5 more
wiley +1 more source
Antrodia cinnamomea reduces obesity and modulates the gut microbiota in high-fat diet-fed mice. [PDF]
, 2018 BackgroundObesity is associated with gut microbiota dysbiosis, disrupted intestinal barrier and chronic inflammation. Given the high and increasing prevalence of obesity worldwide, anti-obesity treatments that are safe, effective and widely available ...
Chang, C-J +11 more
core
GenomeTrakr WGS Protocol Collection and Workflow for MiSeq v2
Here we have created a collection of all the protocols used for WGS using the MiSeq, in order, from sample extraction to NCBI submission. This collection has three sections: Section 1: WGS Wet lab workflow for Illumina MiSeq Section 2: Dry lab workflow for sequence QC and NCBI submission - Direct Submission Section 3: Dry lab workflow for sequence ...
Tina Pfefer +4 more
openaire +1 more source
Integrative Zoology, EarlyView.Avian haemosporidian blood parasites are typically identified through Sanger sequencing of a partial cytochrome b fragment, the MalAvi barcoding region. Next‐generation sequencing is seldom used for avian blood parasite identification; this study demonstrates a higher detection rate of co‐infections via metabarcoding and its possible implications ...
Peter Pibaque +9 more
wiley +1 more source
illumina MiSeq Dual Index Amplicon Sequencing Sample Preparation v1
, 2019 Preparation of PCR products for amplicon sequencing
openaire +1 more source
Microhaplotype Methods Enable Relationship Inference in a Bottlenecked Mammalian Species
Animal Conservation, EarlyView.Northern elephant seals (Mirounga angustirostris) are among the most genetically depauperate mammals in the world. A near‐total population bottleneck in the 19th century, coupled with the breeding system of extreme polygyny, has challenged efforts to estimate individual reproductive success with genetic methods.
Keith M. Hernandez +6 more
wiley +1 more source
Assessment of PHRED Score Characteristics inIllumina MiSeq Amplicon Sequencing
Abstract Background: PHRED scores are confidence values associated with each basecall generated by sequencers. The score is defined as the probability that the basecall is incorrect. The calibration of PHRED scores has been examined in other studies by evaluating errors made in reading known sequences.
Seth Sims +2 more
openaire +1 more source