Results 71 to 80 of about 147,075 (286)

Low-level point heteroplasmy detection in human mitogenomes amplified with different polymerases and sequenced on MiSeq FGx platform

Archives of Forensic Medicine and Criminology, 2023
Introduction: Massively parallel sequencing of mitogenomes usually requires prior amplification.
Katarzyna Skonieczna, Marzanna Ciesielka, Grzegorz Teresiński, Tomasz Grzybowski   +3 more
doaj   +1 more source

Overcoming organic and nitrogen overload in thermophilic anaerobic digestion of pig slurry by coupling a microbial electrolysis cell [PDF]

, 2016
The combination of the anaerobic digestion (AD) process with a microbial electrolysis cell (MEC) coupled to an ammonia stripping unit as a post-treatment was assessed both in series operation, to improve the quality of the effluent, and in loop ...
Bonmatí Blasi, August, Cerrillo Moreno, Míriam, Viñas Canals, Marc   +2 more
core   +2 more sources

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source

Comparison of the performance of MiSeq and NovaSeq in oral microbiome study

Journal of Oral Microbiology
Objective Next generation sequencing is commonly used to characterize the microbiome structure. MiSeq is most commonly used to analyze the microbiome due to its relatively long read length. Illumina also introduced the 250 × 2 chip for NovaSeq.
Hyejung Han, Hyo-Jung Lee, Keun-Suh Kim, Jin Chung, Hee Sam Na   +4 more
doaj   +1 more source

2b-RAD genotyping for population genomic studies of Chagas disease vectors: Rhodnius ecuadoriensis in Ecuador [PDF]

, 2017
Background: Rhodnius ecuadoriensis is the main triatomine vector of Chagas disease, American trypanosomiasis, in Southern Ecuador and Northern Peru.
Andersson, Björn, Costales, Jaime A., De Noia, Michele, Grijalva, Mario J., Hernandez Castro, Luis Enrique, Hernandez-Castro, Luis E., Llewellyn, Martin S., Ocaña-Mayorga, Sofía, Paterno, Marta, Villacís, Anita G., Yumiseva, Cesar A.   +10 more
core   +4 more sources

Bioinformatic Suggestions on MiSeq-Based Microbial Community Analysis

Journal of Microbiology and Biotechnology, 2015
Recent sequencing technology development has revolutionized fields of microbial ecology. MiSeq-based microbial community analysis allows us to sequence more than a few hundred samples at a time, which is far more cost-effective than pyrosequencing. The approach, however, has not been preferably used owing to computational difficulties of processing ...
openaire   +2 more sources

Dietary Baicalin Supplementation Can Enhance the Growth Performance of Weaned Piglets and Maintain the Intestinal Barrier Integrity

Animal Research and One Health, EarlyView.
This research innovatively illustrates baicalin’s multifaceted mechanisms in enhancing piglets’ intestinal health: modulating bile acid metabolism via probiotics, reinforcing tight junction proteins (ZO‐1/claudin), suppressing TLR4/NF‐κB‐mediated inflammation, whereas promoting growth and reducing diarrhea.
Yuhui Gao, Jie Liu, Rui Yang, Xizhen Fan, Cheng Liang, Xin'e Shi, Jianjun Jin   +6 more
wiley   +1 more source

Analysis Comparison for Rapid Identification of Pathogenic Virus from Infected Tissue Samples

Diagnostics, 2022
When examining infectious samples, rapid identification of the pathogenic agent is required for diagnosis and treatment or for investigating the cause of death.
Junji Hosokawa-Muto, Yukiko Sassa-O’Brien, Yoshihito Fujinami, Hiroaki Nakahara   +3 more
doaj   +1 more source

Bacterial Community Sequences of Submerged Aquatic Vegetation in the Potomac River. [PDF]

, 2020
Here, we report results from PCR and sequencing of bacterial 16S rRNA genes from leaf and root surfaces from nine submerged aquatic vegetation (SAV) samples comprising five species.
Alexiev, Alexandra, Eisen, Jonathan A, Lang, Jenna M, Vann, Laura E   +3 more
core  

Metagenomic sequencing unravels gene fragments with phylogenetic signatures of O2-tolerant NiFe membrane-bound hydrogenases in lacustrine sediment [PDF]

, 2015
Many promising hydrogen technologies utilising hydrogenase enzymes have been slowed by the fact that most hydrogenases are extremely sensitive to O2. Within the group 1 membrane-bound NiFe hydrogenase, naturally occurring tolerant enzymes do exist, and ...
Couto, Jillian M., Ijaz, Umer Zeeshan, Phoenix, Vernon R., Schirmer, Melanie, Sloan, William T.   +4 more
core   +1 more source