Results 81 to 90 of about 120,736 (335)

PrimalSeq:Generation of tiled virus amplicons for MiSeq sequencing v1 [PDF]

, 2020
Generated in collaboration by the Loman, Andersen, and Grubaugh labs. For general use of the protocol and primer design, please cite: Quick, J. et al. Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples.
Nate Matteson, Nathan D Grubaugh, Karthik Gangavarapu, Josh Quick, Nick Loman, Kristian Andersen   +5 more
openaire   +1 more source

Saliva microbiome profiling by full-gene 16S rRNA Oxford Nanopore Technology versus Illumina MiSeq sequencing

npj Biofilms and Microbiomes
Molecular characterization of the oral microbiome is a crucial first step in experiments which aim to understand the complex dynamics of the oral microbiome or the interplay with host health and disease.
A. Esberg, N. Fries, Simon Haworth, I. Johansson   +3 more
semanticscholar   +1 more source

Barcoding PCR for MiSeq sequencing v1

, 2018
Barcoding PCR for MiSeq sequencing Barcode head 8mer-GCT ATG CGC GAG CTG C Modified from Rudi et al. (2003)
Eva Petrova, Roey Angel
openaire   +1 more source

Longitudinal genome‐wide aneuploidy measurements in circulating cell‐free DNA to predict lack of benefit from pembrolizumab in patients with metastatic urothelial cancer

Molecular Oncology, EarlyView.
Many patients with urothelial cancer do not benefit from treatment with pembrolizumab, while at risk of severe side effects. Changes in the levels of circulating tumor DNA early during treatment, measured by a simple and affordable assay that can be easily implemented in the clinic, can be used as a prognostic tool to identify these patients.
Youssra Salhi, Stavros Makrodimitris, Sandra van Wilpe, Iris te Paske, Vanja de Weerd, Corine M. Beaufort, Hans M. Westgeest, Jens Voortman, Maureen J. B. Aarts, Maud Rijnders, Astrid A. M. van der Veldt, Ronald de Wit, Niven Mehra, Saskia M. Wilting, Debbie G. J. Robbrecht   +14 more
wiley   +1 more source

High Humidity Exacerbates Psoriasiform Skin Disease Relapse by Increasing Tissue‐Resident Memory T Cells via Altering Skin Microbiota

Advanced Science, EarlyView.
We demonstrated that high humidity worsened psoriasis relapse in murine psoriasiform skin inflammation by increasing skin‐resident memory CD8+ cells via upregulating IL‐15Rα on keratinocytes. The increases in IL‐15Rα and memory CD8+ cells were attributed to S. nepalensis and its metabolite ADMA in skin exposed to high humidity.
Chun‐Ling Liang, Yuchao Chen, Chuanjian Lu, Huazhen Liu, Fenglian Qin, Sisi Dai, Feifei Qiu, Haiming Chen, Weihui Lu, Jonathan S. Bromberg, Zhenhua Dai   +10 more
wiley   +1 more source

Gut Mycobiota‐Associated Tryptophan Catabolites Protect Against Metabolic Dysfunction‐Associated Steatotic Liver Disease

Advanced Science, EarlyView.
ABSTRACT Accumulating evidence suggests that the intestinal microbiota participates in the progression of metabolic dysfunction‐associated steatotic liver disease (MASLD) through microbiota‐host interaction. However, the beneficial role of commensal mycobiota in MASLD progression remains poorly understood.
Shuping Qiao, Shuangya Fan, Juan Xu, Zhen Xu, Chen Peng, Junxing Qu, Ziqian Bing, Shizhen Zhou, Sunan Shen, Guifang Xu, Yue Zhao, Tingting Wang   +11 more
wiley   +1 more source

A method for high precision sequencing of near full-length 16S rRNA genes on an Illumina MiSeq [PDF]

PeerJ, 2016
Background The bacterial 16S rRNA gene has historically been used in defining bacterial taxonomy and phylogeny. However, there are currently no high-throughput methods to sequence full-length 16S rRNA genes present in a sample with precision.
Catherine M. Burke, Aaron E. Darling
doaj   +2 more sources

Detail of corrected positions via MiSeq.

, 2014
Detail of corrected positions via MiSeq.
Sumitaka Hase (645876), Mayumi Kamada (645875), Atsushi Toyoda (24202), Asao Fujiyama (140709), Yasubumi Sakakibara (241039), Kengo Sato (445249)   +5 more
core   +1 more source

Genome‐Wide CRISPR Screen Identifies a microRNA Orchestrating Pleiotropic Resistance to Targeted Therapy and T Cell Immunity in Melanoma

Advanced Science, EarlyView.
A genome‐wide microRNA CRISPR screen identifies miR‐18a as a master regulator of cross‐resistance in melanoma. Loss of miR‐18a activates the AJUBA–YAP/Hippo axis to confer BRAFi resistance and enhances THBS1–CD47 interaction to impair CD8+ T cell immunity. hnRNP A1 is identified as an upstream regulator of miR‐18a processing.
Zhao Wang, Haotian Liu, Xueqi Wang, Jianjin Teng, Zihan Zheng, Jingya Zhang, Wanyi Xiao, Qian Liang, Jiaxin Li, Xiaomeng Jia, Xin Feng, Hongzhang Cui, Menghan Luo, Tielong Yang, Liuxing Wu, Ke Zhao, Weilong Yang, Mulin Jun Li, Dandan Huang, Jilong Yang   +19 more
wiley   +1 more source

Evaluación de la Diversidad Bacteriana de Compostas y Vermicompostas Alimentadas con Desechos Animales. Análisis de Datos Basado en la Secuenciación de Alto Rendimiento de Illumina

Terra Latinoamericana
Los desechos orgánicos animales se aplican comúnmente al suelo para mejorar la fertilidad, pero antes de la aplicación, a menudo se compostan o se vermicompostan para producir un producto más estabilizado, rico en nutrientes y con una amplia diversidad ...
Rocío del Pilar Serrano-Ramírez, Mario Hernández-Guzmán, Víctor Manuel Ruíz-Valdiviezo, Luc Dendooven, Adalberto Zenteno-Rojas, Joaquín Adolfo Montes-Molina   +5 more
doaj   +1 more source