Results 51 to 60 of about 120,736 (335)

A Two-Step PCR Protocol Enabling Flexible Primer Choice and High Sequencing Yield for Illumina MiSeq Meta-Barcoding

open access: yesAgronomy, 2021
High-throughput amplicon sequencing that primarily targets the 16S ribosomal DNA (rDNA) (for bacteria and archaea) and the Internal Transcribed Spacer rDNA (for fungi) have facilitated microbial community discovery across diverse environments.
Ko-Hsuan Chen   +3 more
semanticscholar   +1 more source

A shared mucosal gut microbiota signature in primary sclerosing cholangitis before and after liver transplantation

open access: yesHepatology, EarlyView., 2022
In this study of the gut mucosal microbiota in primary sclerosing cholangitis (PSC), we found consistent microbiota features associated with PSC and recurrent PSC, PSC with inflammatory bowel disease and a persistent gut dysbiosis after liver transplantation Abstract Background and Aims Several characteristic features of the fecal microbiota have been ...
Mikal Jacob Hole   +12 more
wiley   +1 more source

Complete mitogenomes of five ecologically diverse Australian freshwater fishes

open access: yesMitochondrial DNA. Part B. Resources, 2019
Complete mitochondrial genome sequences were determined for five species of Australian freshwater fishes, representing a diverse range of ecologies and life histories. Mitogenomes were sequenced and annotated for Craterocephalus stramineus (Atherinidae);
Daniel J. Schmidt, Carmel McDougall
doaj   +1 more source

Comparison of Sequencing Methods for Obtaining a Whole Genome of Pea Necrotic Yellow Dwarf Virus from U.K. Peas

open access: yesPhytoFrontiers, 2023
During a project investigating pea viruses in the United Kingdom and developing a workflow for high-throughput sequencing in crop surveillance, pea necrotic yellow dwarf virus (PNYDV) was identified at one site, along with pea enation mosaic virus-1, pea
A. R. Fowkes   +14 more
doaj   +1 more source

An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform

open access: yesMicrobiome, 2014
To take advantage of affordable high-throughput next-generation sequencing technologies to characterize microbial community composition often requires the development of improved methods to overcome technical limitations inherent to the sequencing ...
D. Fadrosh   +6 more
semanticscholar   +1 more source

Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform

open access: yesPLOS ONE, 2017
Illumina's MiSeq has become the dominant platform for gene amplicon sequencing in microbial ecology studies; however, various technical concerns, such as reproducibility, still exist. To assess reproducibility, 16S rRNA gene amplicons from 18 soil samples of a reciprocal transplantation experiment were sequenced on an Illumina MiSeq.
Chongqing Wen   +10 more
openaire   +6 more sources

A Comparison between Transcriptome Sequencing and 16S Metagenomics for Detection of Bacterial Pathogens in Wildlife. [PDF]

open access: yesPLoS Neglected Tropical Diseases, 2015
BACKGROUND:Rodents are major reservoirs of pathogens responsible for numerous zoonotic diseases in humans and livestock. Assessing their microbial diversity at both the individual and population level is crucial for monitoring endemic infections and ...
Maria Razzauti   +8 more
doaj   +1 more source

A quantitative and qualitative comparison of illumina MiSeq and 454 amplicon sequencing for genotyping the highly polymorphic major histocompatibility complex (MHC) in a non-model species

open access: yesBMC Research Notes, 2017
Background High-throughput sequencing enables high-resolution genotyping of extremely duplicated genes. 454 amplicon sequencing (454) has become the standard technique for genotyping the major histocompatibility complex (MHC) genes in non-model organisms.
Haslina Razali   +4 more
doaj   +1 more source

Prepare Samples for Miseq v1

open access: yes, 2023
(MiSeq nano V2 chip 300 cycle), MiSeq multiplexes sequences, allowing you to DNA sequence multiple samples at once.
openaire   +1 more source

Perspectives of using Illumina MiSeq for identification of arbuscular mycorrhizal fungi

open access: yesVavilov Journal of Genetics and Breeding, 2020
Arbuscular mycorrhiza fungi (AMF) form one of the most common symbiosis with the majority of land plants. AMF supply the plant with various mineral elements, primarily phosphorus, and improve the water supply. The search for the most effective AMF strains for symbiosis and the creation of microbial preparations on that basis is an important task for ...
A. A. Kryukov   +6 more
openaire   +4 more sources

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