Results 41 to 50 of about 102,184 (119)

Illumina MiSeq sequencing analysis of fungal diversity in stored dates

BMC Microbiology, 2017
Background Date palm has been a major fruit tree in the Middle East over thousands of years, especially in the Arabian Peninsula. Dates are consumed fresh (Rutab) or after partial drying and storage (Tamar) during off-season.
Ismail M. Al-Bulushi, Muna S. Bani-Uraba, Nejib S. Guizani, Mohammed K. Al-Khusaibi, Abdullah M. Al-Sadi   +4 more
doaj   +1 more source

A MiSeq-HyDRA platform for enhanced HIV drug resistance genotyping and surveillance

Scientific Reports, 2019
Conventional HIV drug resistance (HIVDR) genotyping utilizes Sanger sequencing (SS) methods, which are limited by low data throughput and the inability of detecting low abundant drug resistant variants (LADRVs).
Tracy Taylor, Emma R. Lee, Mikaela Nykoluk, Eric Enns, Binhua Liang, Rupert Capina, Marie-Krystel Gauthier, Gary Van Domselaar, Paul Sandstrom, James Brooks, Hezhao Ji   +10 more
doaj   +1 more source

Quasispecies Analyses of the HIV-1 Near-full-length Genome with Illumina MiSeq

Frontiers in Microbiology, 2015
Human immunodeficiency virus type-1 (HIV-1) exhibits high between-host genetic diversity and within-host heterogeneity, recognized as quasispecies. Because HIV-1 quasispecies fluctuate in terms of multiple factors, such as antiretroviral exposure and ...
Hirotaka eOde, Masakazu eMatsuda, Kazuhiro eMatsuoka, Atsuko eHachiya, Junko eHattori, Yumiko eKito, Yoshiyuki eYokomaku, Yasumasa eIwatani, Yasumasa eIwatani, Wataru eSugiura, Wataru eSugiura   +10 more
doaj   +1 more source

Fungal Community Structure of Macroalga Ulva Intestinalis Revealed by Miseq Sequencing

Squalen, 2017
Since algae are well known as the host of fungi, the information about total fungal community associated with alga is needed to give the initial data that is useful for natural product discovery. The study on total fungal community associated with marine
Agusman Agusman, Feng Danqing
doaj   +1 more source

Novel rhesus macaque immunoglobulin germline genes identified by three sequencing approaches

Frontiers in Immunology
IntroductionRhesus macaques have long been a focus of research for understanding immune responses to human pathogens due to their close phylogenetic relationship with humans.
Yicheng Guo, Yicheng Guo, Eric Waltari, Hong Lu, Hong Lu, Zizhang Sheng, Zizhang Sheng, Xueling Wu, Xueling Wu   +8 more
doaj   +1 more source

Whole-genome and targeted sequencing of drug-resistant Mycobacterium tuberculosis on the iSeq100 and MiSeq: A performance, ease-of-use, and cost evaluation.

PLoS Medicine, 2019
BackgroundAccurate, comprehensive, and timely detection of drug-resistant tuberculosis (TB) is essential to inform patient treatment and enable public health surveillance. This is crucial for effective control of TB globally. Whole-genome sequencing (WGS)
Rebecca E Colman, Aurélien Mace, Marva Seifert, Jonathan Hetzel, Haifa Mshaiel, Anita Suresh, Darrin Lemmer, David M Engelthaler, Donald G Catanzaro, Amanda G Young, Claudia M Denkinger, Timothy C Rodwell   +11 more
doaj   +1 more source

Performance comparison of bench-top next generation sequencers using microdroplet PCR-based enrichment for targeted sequencing in patients with autism spectrum disorder.

PLoS ONE, 2013
Next-generation sequencing (NGS) combined with enrichment of target genes enables highly efficient and low-cost sequencing of multiple genes for genetic diseases.
Eriko Koshimizu, Satoko Miyatake, Nobuhiko Okamoto, Mitsuko Nakashima, Yoshinori Tsurusaki, Noriko Miyake, Hirotomo Saitsu, Naomichi Matsumoto   +7 more
doaj   +1 more source

Comparative analyses of the results of HLA genes typing by NGS method using different platforms

Медицинская иммунология
Next generation sequencing (NGS) allows high-resolution and allelic HLA-typing. The most common platform is MiSeq (Illumina, USA). Currently, the maintenance of this device is difficult, which has necessitated the search for analogues that are not ...
I. E. Pavlova, E. V. Kuzmich, E. G. Khamaganova, D. V. Rudik, E. P. Kuzminova, A. R. Abdrakhimova   +5 more
doaj   +1 more source

Sequencing of 231 forensic genetic markers using the MiSeq FGx™ forensic genomics system – an evaluation of the assay and software

Forensic Sciences Research, 2018
The MiSeq FGx™ Forensic Genomics System types 231 genetic markers in one multiplex polymerase chain reaction (PCR) assay. The markers include core forensic short tandem repeats (STRs) as well as identity, ancestry and phenotype informative short ...
Christian Hussing, Christina Huber, Rajmonda Bytyci, Helle S. Mogensen, Niels Morling, Claus Børsting   +5 more
doaj   +1 more source

Comparison of Two Massively Parallel Sequencing Platforms using 83 Single Nucleotide Polymorphisms for Human Identification

Scientific Reports, 2017
The potential of Massively Parallel Sequencing (MPS) technology to vastly expand the capabilities of human identification led to the emergence of different MPS platforms that use forensically relevant genetic markers.
Dame Loveliness T. Apaga, Sheila E. Dennis, Jazelyn M. Salvador, Gayvelline C. Calacal, Maria Corazon A. De Ungria   +4 more
doaj   +1 more source