Results 21 to 30 of about 120,736 (335)

Mitochondrial genome sequencing with short overlapping amplicons on MiSeq FGx system

Forensic Sciences Research, 2021
With the development and maturation of massively parallel sequencing (MPS) technology, the mitochondrial genome (mitogenome) sequencing is increasingly applied in the forensic field.
Yang Xin, Rulin Jia, Suhua Zhang, Fei Guo   +3 more
doaj   +3 more sources

Assessment of PHRED Score Characteristics in Illumina MiSeq Amplicon Sequencing. [PDF]

J Comput Biol
PHRED scores are confidence values associated with each basecall generated by sequencers. The score is defined as a monotonic function of the probability that the basecall is incorrect. The calibration of PHRED scores has previously been examined by evaluating errors made in reading known sequences.
Sims S, Khudyakov Y, Zelikovsky A.
europepmc   +2 more sources

Investigation of MiSeq reproducibility on biomarker identification [PDF]

Applied Biological Chemistry, 2019
Abstract MiSeq-derived artificial sequences appeared to be of good quality, thus bioinformatics tools failed to remove MiSeq artefacts. Even after removing singleton sequences or operational taxonomic units (OTUs), it is not clear how many sequence artefacts remained.
Hyejun Jo, Jiwan Hong, Tatsuya Unno
openaire   +1 more source

Gut microbiome profiling of neonates using Nanopore MinION and Illumina MiSeq sequencing

Frontiers in Microbiology, 2023
This study aimed to evaluate the difference in gut microbiomes between preterm and term infants using third-generation long-read sequencing (Oxford Nanopore Technologies, ONT) compared with an established gold standard, Illumina (second-generation short ...
Teahyen Cha, Hoo Hugo Kim, J. Keum, M. Kwak, Jae Yong Park, J. Hoh, Chang-Ryul Kim, Byong-hun Jeon, Hyun-Kyung Park   +8 more
semanticscholar   +1 more source

Characterization of the complete mitochondrial genome of the Northern Mud Gudgeon, Ophiocara porocephala (Perciformes: Eleotridae) with phylogenetic implications

Mitochondrial DNA. Part B. Resources, 2021
The first mitochondrial genome of Ophiocara porocephala was determined by the combination of next-generation sequencing (NGS) and Sanger sequencing methods. A complete circular mitogenome of O. porocephala (16,529 bp) consisted of 13 protein-coding genes,
Muhammad Hilman Fu’adil Amin, Soo Rin Lee, Bambang Irawan, Sapto Andriyono, Hyun-Woo Kim   +4 more
doaj   +1 more source

Rumen Epithelial Communities Share a Core Bacterial Microbiota: A Meta-Analysis of 16S rRNA Gene Illumina MiSeq Sequencing Datasets

Frontiers in Microbiology, 2021
In this meta-analysis, 17 rumen epithelial 16S rRNA gene Illumina MiSeq amplicon sequencing data sets were analyzed to identify a core rumen epithelial microbiota and core rumen epithelial OTUs shared between the different studies included.
C. Anderson, Lucas R. Koester, S. Schmitz-Esser   +2 more
semanticscholar   +1 more source

Validation and beyond: Next generation sequencing of forensic casework samples including challenging tissue samples from altered human corpses using the MiSeq FGx system

Journal of Forensic Sciences, 2022
The proceeding developments in next generation sequencing (NGS) technologies enable increasing discrimination power for short tandem repeat (STR) analyses and provide new possibilities for human identification. Therefore, the growing relevance and demand
Alina Senst, A. Caliebe, E. Scheurer, I. Schulz   +3 more
semanticscholar   +1 more source

Comparison of iSeq and MiSeq as the two platforms for 16S rRNA sequencing in the study of the gut of rat microbiome

Applied Microbiology and Biotechnology, 2022
Amplicon-based next-generation sequencing (NGS) of the 16S ribosomal RNA (16S) regions is a culture-free method used to identify and analyze Procaryota occurring within a given sample.
D. Salamon, B. Zapała, A. Krawczyk, A. Potasiewicz, A. Nikiforuk, A. Stój, T. Gosiewski   +6 more
semanticscholar   +1 more source

Ethnic Differences Shape the Alpha but Not Beta Diversity of Gut Microbiota from School Children in the Absence of Environmental Differences

Microorganisms, 2020
Although the human gut microbiome is shaped by factors such as diet, environment, and genetic background, most studies investigating the relationship between ethnicity and microbiota have compared groups living in separate geographical locations.
Ke Liu, Yongling Zhang, Qinglin Li, Huan Li, Danfeng Long, Shijuan Yan, Wenjie Huang, Ruijun Long, Xiaodan Huang   +8 more
doaj   +1 more source

Evaluation of the VISAGE basic tool for appearance and ancestry inference using ForenSeq® chemistry on the MiSeq FGx® system.

Forensic Science International: Genetics, 2022
The possibility of providing investigative leads when conventional DNA identification methods fail to solve a case can be of extreme relevance to law enforcement.
C. Xavier, M. de la Puente, M. Sidstedt, K. Junker, Angelika Minawi, M. Unterländer, Y. Chantrel, François-Xavier Laurent, Anna Delest, C. Hohoff, I. Bastisch, J. Hedman, K. J. van der Gaag, T. Sijen, W. Parson   +14 more
semanticscholar   +1 more source