Results 1 to 10 of about 120,736 (335)

Comparison of the performance of MiSeq and NovaSeq in oral microbiome study

open access: yesJournal of Oral Microbiology
Objective Next generation sequencing is commonly used to characterize the microbiome structure. MiSeq is most commonly used to analyze the microbiome due to its relatively long read length. Illumina also introduced the 250 × 2 chip for NovaSeq.
, Keun-Suh Kim, Jin Chung
exaly   +7 more sources

Comparison of Oxford Nanopore Technologies and Illumina MiSeq sequencing with mock communities and agricultural soil

open access: yesScientific Reports, 2023
Illumina MiSeq is the current standard for characterizing microbial communities in soil. The newer alternative, Oxford Nanopore Technologies MinION sequencer, is quickly gaining popularity because of the low initial cost and longer sequence reads ...
Bo Maxwell Stevens   +3 more
doaj   +3 more sources

Genomic analyses of multidrug-resistant Salmonella Indiana, Typhimurium, and Enteritidis isolates using MinION and MiSeq sequencing technologies.

open access: yesPLoS ONE, 2020
We sequenced 25 isolates of phenotypically multidrug-resistant Salmonella Indiana (n = 11), Typhimurium (n = 8), and Enteritidis (n = 6) using both MinION long-read [SQK-LSK109 and flow cell (R9.4.1)] and MiSeq short-read (Nextera XT and MiSeq Reagent ...
Zhao Chen   +6 more
doaj   +3 more sources

A MiSeq-HyDRA platform for enhanced HIV drug resistance genotyping and surveillance [PDF]

open access: yesScientific Reports, 2019
Conventional HIV drug resistance (HIVDR) genotyping utilizes Sanger sequencing (SS) methods, which are limited by low data throughput and the inability of detecting low abundant drug resistant variants (LADRVs).
Tracy Taylor   +10 more
doaj   +4 more sources

Comparison of SARS-CoV-2 sequencing using the ONT GridION and the Illumina MiSeq

open access: yesBMC Genomics, 2022
Background Over 4 million SARS-CoV-2 genomes have been sequenced globally in the past 2 years. This has been crucial in elucidating transmission chains within communities, the development of new diagnostic methods, vaccines, and antivirals.
Derek Tshiabuila   +12 more
doaj   +2 more sources

Conservation and diversity of the pollen microbiome of Pan-American maize using PacBio and MiSeq

open access: yesFrontiers in Microbiology, 2023
Pollen is a vector for diversification, fitness-selection, and transmission of plant genetic material. The extent to which the pollen microbiome may contribute to host diversification is largely unknown, because pollen microbiome diversity within a plant
Eman M Khalaf   +2 more
exaly   +2 more sources

Developmental validation of the ForenSeq MainstAY kit, MiSeq FGx sequencing system and ForenSeq Universal Analysis Software

open access: yesForensic Science International: Genetics, 2023
For human identification purposes, forensic genetics has primarily relied upon a core set of autosomal (and to a lesser extent Y chromosome) short tandem repeat (STR) markers that are enriched by amplification using the polymerase chain reaction (PCR ...
Bobby Larue, Bruce Budowle
exaly   +2 more sources

Whole-genome and targeted sequencing of drug-resistant Mycobacterium tuberculosis on the iSeq100 and MiSeq: A performance, ease-of-use, and cost evaluation.

open access: yesPLoS Medicine, 2019
BackgroundAccurate, comprehensive, and timely detection of drug-resistant tuberculosis (TB) is essential to inform patient treatment and enable public health surveillance. This is crucial for effective control of TB globally. Whole-genome sequencing (WGS)
Rebecca E Colman   +11 more
doaj   +2 more sources

Deciphering Azadirachta indica (Neem) Gum Microbiome using Metagenomic Approaches

open access: yesJournal of Pure and Applied Microbiology, 2023
Indian lilac or neem (Azadirachta indica) is found in tropical and subtropical regions of the Indian subcontinent. Each part of the tree is a source of various phytochemicals. Neem gum is an exudate from mature parts of the plant stem.
Pragya Saxena   +4 more
doaj   +1 more source

MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species [PDF]

open access: yesRoyal Society Open Science, 2015
We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using aligned whole mitochondrial genome (mitogenome) sequences from 880 species, supplemented by partial mitogenome ...
M. Miya   +11 more
doaj   +1 more source

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