Results 1 to 10 of about 102,184 (119)

A quantitative and qualitative comparison of illumina MiSeq and 454 amplicon sequencing for genotyping the highly polymorphic major histocompatibility complex (MHC) in a non-model species

BMC Research Notes, 2017
Background High-throughput sequencing enables high-resolution genotyping of extremely duplicated genes. 454 amplicon sequencing (454) has become the standard technique for genotyping the major histocompatibility complex (MHC) genes in non-model organisms.
Haslina Razali, Emily O’Connor, Anna Drews, Terry Burke, Helena Westerdahl   +4 more
doaj   +1 more source

Rapid and economical drug resistance profiling with Nanopore MinION for clinical specimens with low bacillary burden of Mycobacterium tuberculosis

BMC Research Notes, 2020
Objective We designed and tested a Nanopore sequencing panel for direct tuberculosis drug resistance profiling. The panel targeted 10 resistance-associated loci.
Wai Sing Chan, Chun Hang Au, Yvonne Chung, Henry Chi Ming Leung, Dona N. Ho, Elaine Yue Ling Wong, Tak Wah Lam, Tsun Leung Chan, Edmond Shiu Kwan Ma, Bone Siu Fai Tang   +9 more
doaj   +1 more source

Hybrid De Novo Genome Assembly Using MiSeq and SOLiD Short Read Data. [PDF]

PLoS ONE, 2015
A hybrid de novo assembly pipeline was constructed to utilize both MiSeq and SOLiD short read data in combination in the assembly. The short read data were converted to a standard format of the pipeline, and were supplied to the pipeline components such ...
Tsutomu Ikegami, Toyohiro Inatsugi, Isao Kojima, Myco Umemura, Hiroko Hagiwara, Masayuki Machida, Kiyoshi Asai   +6 more
doaj   +1 more source

Evaluation of metagenetic community analysis of planktonic copepods using Illumina MiSeq: Comparisons with morphological classification and metagenetic analysis using Roche 454.

PLoS ONE, 2017
Metagenetics is a rapid and taxonomically comprehensive method for revealing community structures within environmental samples, based on large amounts of sequence data produced by high-throughput sequencers.
Junya Hirai, Satoshi Nagai, Kiyotaka Hidaka   +2 more
doaj   +1 more source

Improved High-Throughput Sequencing of the Human Oral Microbiome: From Illumina to PacBio

Canadian Journal of Infectious Diseases and Medical Microbiology, 2020
Background. A comprehensive understanding of the commensal microflora and its relation to health is essential for preventing and combating diseases. The aim of this study was to examine the structure of the oral microbiome by using different sequencing ...
Jie Zhang, Lingkai Su, Yuan Wang, Shuli Deng   +3 more
doaj   +1 more source

Insights into bacterioplankton community structure from Sundarbans mangrove ecoregion using Sanger and Illumina MiSeq sequencing approaches: A comparative analysis

Genomics Data, 2017
Next generation sequencing using platforms such as Illumina MiSeq provides a deeper insight into the structure and function of bacterioplankton communities in coastal ecosystems compared to traditional molecular techniques such as clone library approach ...
Anwesha Ghosh, Punyasloke Bhadury
doaj   +1 more source

Comparative next-generation sequencing of adeno-associated virus inverted terminal repeats

BioTechniques, 2014
The inverted terminal repeats (ITRs) of adeno-associated virus (AAV) are notoriously difficult to sequence owing to their high GC-content (70%) and palindromic sequences that result in the formation of a very stable, 125 bp long, T-shaped hairpin ...
Karl Petri, Raffaele Fronza, Richard Gabriel, Christine Käppel, Ali Nowrouzi, R. Michael Linden, Els Henckaerts, Manfred Schmidt   +7 more
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Transmitting silks of maize have a complex and dynamic microbiome

Scientific Reports, 2021
In corn/maize, silks emerging from cobs capture pollen, and transmit resident sperm nuclei to eggs. There are > 20 million silks per U.S. maize acre.
Eman M. Khalaf, Anuja Shrestha, Jeffrey Rinne, Michael D. J. Lynch, Charles R. Shearer, Victor Limay-Rios, Lana M. Reid, Manish N. Raizada   +7 more
doaj   +1 more source

Effects of wheat root exudates on bacterial communities in the rhizosphere of watermelon

Plant, Soil and Environment, 2021
In this study, we investigated the effects of wheat root exudates on soil bacterial communities in the watermelon rhizosphere using quantitative PCR and Illumina MiSeq sequencing.
Jibo Shi, Xiaoya Gong, Muhammad Khashi u Rahman, Qing Tian, Xingang Zhou, Fengzhi Wu   +5 more
doaj   +1 more source

Next-generation sequencing-based multigene mutational screening for acute myeloid leukemia using MiSeq: applicability for diagnostics and disease monitoring

Haematologica, 2014
Routine molecular testing in acute myeloid leukemia involves screening several genes of therapeutic and prognostic significance for mutations. A comprehensive analysis using single-gene assays requires large amounts of DNA, is cumbersome and timely ...
Rajyalakshmi Luthra, Keyur P. Patel, Neelima G. Reddy, Varan Haghshenas, Mark J. Routbort, Michael A. Harmon, Bedia A. Barkoh, Rashmi Kanagal-Shamanna, Farhad Ravandi, Jorge E Cortes, Hagop M Kantarjian, L. Jeffrey Medeiros, Rajesh R. Singh   +12 more
doaj   +1 more source